Site-directed immobilisation of antibody fragments for detection of C-reactive protein

Vikholm-Lundin, Inger; Albers, Willem M.

doi:10.1016/j.bios.2005.04.011

Cited by 78 publications

(55 citation statements)

References 30 publications

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“…AuNPs on the surface have increased the sensitivity of the sensor. This method is more sensitive compared with other measurement methods based on related data have shown that (Meyer et al, 2002, Meyer et al, 2007Vikholm-Lundin et al, 2006). 87fM be determined until this study, low-range signal.…”

Section: Qcm Based Biosensors Developed For Determination Of Crpmentioning

confidence: 79%

Biosensors for Cancer Biomarkers

Uygun¹,

Sezgintürk²

2011

Biosensors - Emerging Materials and Applications

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Section: Qcm Based Biosensors Developed For Determination Of Crpmentioning

confidence: 79%

Biosensors for Cancer Biomarkers

Uygun¹,

Sezgintürk²

2011

Biosensors - Emerging Materials and Applications

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“…Enzymatic digestion of an Ab followed by chemical reduction has yielded Fab fragments with exposed active sulfhydryl groups. Direct bonding between the sulfhydryl group and a gold surface, as well as thiol-specific cross-linking on non-metallic surfaces has been reported [20,21].…”

Section: Fig (3)mentioning

confidence: 98%

Trends and Perspectives in Immunosensors

2012

Antibodies Applications and New Developments

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Immunosensors are devices that comprise both a biomolecular recognition system, such as an antibody and its corresponding antigen, and a transducer to translate the high affinity and specific binding event into a physical signal.Antibodies are produced by an immunological response to the presence of a foreign substance called an antigen. Antibodies may be immobilised onto a variety of platforms including bulk planar surfaces and nanoparticles by either covalent or adsorption strategies. Different interfaces between the bio-components and the detector are available to monitor in 'real-time' the signal generated by biological interactions. The transducers detect, for example, the change in electron transfer, absorbance, fluorescence, refractive index, mass change or heat transfer as the antibody binds to the antigen of interest. Such analytical devices have allowed a wide range of analytes to be identified and quantified such as pathogens, toxins, environmental food contaminants and disease biomarkers.The demand for sensitive, rapid, 'on-site' techniques has taken advantage of the latest advances in microfluidics and nanotechnology. This chapter will highlight current trends in immobilisation, micro/nano-fluidics/ and transducers utilised. A number of examples outlining the exploitation of these elements in immunosensors and their successful applications will be described.

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“…The non-ionic hydrophilic polymer of N-[tris(hydroxymethyl)methyl]acrylamide, pTHMMAA used to suppress non-specific binding to antibody layers were prepared as previously described (Vikholm-Lundin and Albers, 2006). Recombinant anti-morphine M1 antibody Fab fragments has been enriched from phage display library as described previously (Pulli et al, 2005).…”

Section: Production Of Lipoate Polymer and Recombinant Anti-morphinementioning

confidence: 99%

“…Coupling of the antibody Fab fragments onto the sensor surface is simple and fast and can be used for attaching any antibody in an oriented manner to the sensor surface. The immobilisation method has also been demonstrated for antibody fragments specific for C reactive protein (Vikholm-Lundin and Albers, 2006). The layer shows a 5-fold higher response to antigen than antibody F(ab) 2 -fragments that are randomly adsorbed on the sensor surface.…”

Section: Introductionmentioning

confidence: 96%