Sitagliptin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in Aneurysm Necks Through the SDF-1/CXCR4/NRF2 Signaling Pathway

Yu, Guangrong; Liu, Peixi; Shi, Yuan; Li, Sichen; Liu, Yingjun; Zhu, Wei

doi:10.3389/fendo.2019.00823

Cited by 13 publications

(6 citation statements)

References 30 publications

(31 reference statements)

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“…SDF-1 is a chemokine that has a strong chemotactic effect on EPCs. After vascular injury, SDF-1 induces migration of EPCs to the injured area and promotes endothelialization [20,21]. Under hyperglycemic conditions miR-133a was found to be up-regulated and induced endothelial dysfunction through inhibition of eNOS [22].…”

Section: Discussionmentioning

confidence: 97%

miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease

Lee

Lin

2020

Preprint

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Background: Peripheral artery disease (PAD) is a vascular disease involving diffuse atherosclerosis, which is associated with increased cardiovascular mortality and morbidity. Critical limb ischemia is the most severe complication of PAD. In addition to medical and interventional treatment, therapeutic angiogenesis is a novel therapy for PAD. Circulating microRNAs (miRNAs) are considered to be key regulators of gene expression, but their role in ischemic-induced angiogenesis is poorly characterized. There is little knowledge of specific miRNAs associated with PAD. Methods: To determine the regulation of miRNAs, we obtained miRNA profiles using RNA isolated from patients with PAD and a control group. The effect of the specific miRNA in angiogenesis were evaluated by in vitro angiogenic function of endothelial progenitor cells (EPCs), in vivo angiogenesis assay and a hind-limb ischemic model. Results: Circulating miR-548j-5p was significantly reduced in patients with PAD compared with the controls. miR-548j-5p promoted EPC angiogenesis by enhancing migration and tube formation. The endothelial nitric oxide synthase and stromal cell-derived factor (SDF)-1 signaling pathways appeared to be potential targets for miR-548j-5p. Furthermore, a directed in vivo angiogenesis assay of EPCs and a hind-limb ischemia mouse model demonstrated that miR-548j-5p enhanced capillary density and blood flow recovery in hind-limb ischemia. Conclusion: Taken together, our data indicates that up-regulation of miR-548j-5p promotes angiogenesis in ischemic tissue and might be a novel therapeutic approach for PAD.

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Section: Discussionmentioning

confidence: 97%

miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease

Lee

Lin

2020

Preprint

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“…SDF-1 is a chemokine that has a strong chemotactic effect on EPCs. After vascular injury, SDF-1 induces migration of EPCs to the injured area and promotes endothelialization 32 , 33 . Under hyperglycemic conditions, miR-133a was found to be up-regulated, and induced endothelial dysfunction through inhibition of eNOS 34 .…”

Section: Discussionmentioning

confidence: 99%

miR-548j-5p regulates angiogenesis in peripheral artery disease

Lee

Lin

2022

Sci Rep

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Peripheral artery disease (PAD) is a vascular disease involving diffuse atherosclerosis, and is associated with increased cardiovascular mortality and morbidity. Critical limb ischemia (CLI) is the most severe complication of PAD. In addition to medical and interventional treatment, therapeutic angiogenesis is a novel therapy for PAD. Circulating microRNAs (miRNAs) are considered key regulators of gene expression, but their role in ischemic-induced angiogenesis is poorly-characterized. There is currently a limited understanding of the specific miRNAs associated with PAD. To determine the regulation of miRNAs, we obtained miRNA profiles using RNA isolated from patients with PAD and a control group. The effects of specific miRNAs on angiogenesis were evaluated by assessing the in vitro angiogenic function of endothelial progenitor cells (EPCs), performing an in vivo angiogenesis assay, and employing a mouse hindlimb ischemic model. Our results demonstrated that circulating miR-548j-5p was significantly reduced in patients with PAD as compared with the controls. miR-548j-5p promoted EPC angiogenesis by enhancing migration and tube formation. The endothelial nitric oxide synthase (NOS) and stromal cell-derived factor (SDF)-1 signaling pathways appeared to be potential targets of miR-548j-5p. Furthermore, the results of a directed in vivo angiogenesis assay of EPCs and a hindlimb ischemia mouse model demonstrated that miR-548j-5p enhanced the capillary density and blood flow recovery in hindlimb ischemia. In conclusion, our data indicated that up-regulation of miR-548j-5p promotes angiogenesis in ischemic tissue and may represent a novel therapeutic approach for PAD.

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“…Wang et al confirmed that metallothionein (MT) promotes the angiogenesis of EPCs mediated by the HIF-1 α /SDF-1/VEGF pathway [ 54 ]. Several studies proved that the SDF-1/VEGF pathway is vital to the angiogenesis of EPCs [ 55 , 56 ]. In our research, the expressions of the angiogenesis-related genes CD34, VEGF, MMP9, and SDF-1 were examined and found to be upregulated at an appropriate concentration of GO.…”

Section: Discussionmentioning

confidence: 99%

Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs

Zhang

Shi³

et al. 2021

Stem Cells International

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Angiogenesis in the field of tissue engineering has attracted significant attention. Graphene oxide has become a promising nanomaterial in tissue engineering for its unique biochemical properties. Therefore, herein, a series of chitosan (CS)/graphene oxide (GO) hydrogel scaffolds were synthesized by crosslinking CS and GO at different concentrations (0.1, 0.5, and 1.0 wt.%) using genipin. Compared with the CS hydrogel scaffolds, the CS/GO hydrogel scaffolds have a better network structure and mechanical strength. Then, we used endothelial progenitor cells (EPCs) extracted from human umbilical cord blood and cocultured these EPCs with the as-prepared scaffolds. The scaffolds with 0.1 and 0.5 wt.%GO showed no considerable cytotoxicity, could promote the proliferation of EPCs and tube formation, and upregulated the expressions of CD34, VEGF, MMP9, and SDF-1 in EPCs compared to the case of the scaffold with 1.0 wt.%GO. This study shows that the addition of graphene oxide improves the structure of chitosan hydrogel and enhances the proliferation activity and angiogenic capacity of EPCs.

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Sitagliptin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in Aneurysm Necks Through the SDF-1/CXCR4/NRF2 Signaling Pathway

Cited by 13 publications

References 30 publications

miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease

miR-548j-5p Regulates Angiogenesis in Peripheral Artery Disease

miR-548j-5p regulates angiogenesis in peripheral artery disease

Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs

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