Sister Chromatid Exchanges, Indices of Human Chromosome Damage and Repair: Detection by Fluorescence and Induction by Mitomycin C

Latt, Samuel A.

doi:10.1073/pnas.71.8.3162

Cited by 381 publications

(93 citation statements)

References 24 publications

(18 reference statements)

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“…Sontakke YA (2008) 1 found that interchange exchange was the most common chromosomal aberration induced by mitomycin c and other chromosomal aberrations were associations of acrocentric association and attenuation of secondary constriction and agrees with the present study. Latt SA (1974) 28 observed significant increase in the SCE (sister chromatid exchange) with increase in concentration of mitomycin c. Finding of the above researchers corresponds with the present study. Brogger A (1973) 25 revealed a prevalence of attenuations, lateral extensions and exchanges in the secondary constrictions of chromosomes 1, 9, and 16 in the mitomycin c treated material and not with methylmetanesulphonate MMS.…”

Section: Interchange Exchange By Mitomycin Csupporting

confidence: 80%

Genotixicity of Mitocycin C – Cytogenetic Study

Fulmali¹

2017

IOSR JDMS

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Abstract:The present study was undertaken to study genotoxic effect of Mitomycin C at different concentrations for different duration on human chromosomes in search of a dose which will be more effective and less toxic in lymphocytic culture and to find the specificity of its action on human chromosomes in lymphocyte culture. The range of concentration of Mitomycin C (0.05, 0.1, 0.5, 1.0, 2.0, 5.0

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Section: Interchange Exchange By Mitomycin Csupporting

confidence: 80%

Genotixicity of Mitocycin C – Cytogenetic Study

Fulmali¹

2017

IOSR JDMS

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“…Likewise, the observation of SCE notably indicates effects of chemical mutagens and carcinogens on eukaryotic chromosomes. The first study dealing with this issue was carried out by Latt (1974) on human lymphocyte chromosomes treated with the bifunctional alkylating agent MMC that cross-links DNA. Additionally, Perry and Evans (1975) found that all direct-acting mutagens led to dramatically increased frequencies of SCEs in Chinese hamster ovary cells treated with 14 known or suspected mutagens/carcinogens.…”

Section: Discussionmentioning

confidence: 99%

In vitro genotoxic perspective of Tamiflu

İla¹,

İlhan²

2012

Cytotechnology

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The aim of this study was to investigate the genotoxic and/or cytotoxic effects of Tamiflu, commercial form of the oseltamivir antiviral and most frequently prescribed for the treatment of influenza infections, on cultured human peripheral lymphocytes by using sister chromatid exchange (SCE), chromosomal aberration (CA), and cytokinesis-blocked micronucleus (CBMN) assays. Cells were treated with 0.5, 1, 2 lg/mL oseltamivir, the Tamiflu capsule ingredient, for 24 or 48 h in the absence or presence of an exogenous metabolic activation system (S9 mix). The test chemical did not demonstrate any genotoxic effect dose-dependently but it showed a weak cytotoxicity on cells in this study. On the other hand, some concentrations of Tamiflu (2 lg/mL without S9 mix for 48 h and 1 lg/mL with S9 mix) induced SCE and also decreased significantly the proliferation index (PI) (48 h period) and the nuclear division index (NDI) (24 h period) (P \ 0.05) in the absence of S9 mix. Considering the results, Tamiflu did not induce significant increases of CA or micronucleated cells in vitro in cultured peripheral blood lymphocytes under the treatment conditions used but weak SCE induction was observed. On the other hand, the weak cytotoxic effects observed disappeared in the cultures treated in presence of the S9 mix.

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“…This would inhibit DNA replication and probably might be the antitumor action of this drug. Despite the fact that MMC showed antitumor action, many reports proved that it may cause high frequency of chromosomal aberrations, sister chromatid exchanges and micronuclei formation (Latt 1974;Fauth et al 2000;Yogesh and Fulzele 2009). In this study, we used this antitumor-antibiotic drug to test the possible antimutagenic activity of concrete and absolute oils.…”

Section: Gc-ms Analysismentioning

confidence: 99%

Cytogenetic, cytotoxic and GC–MS studies on concrete and absolute oils from Taif rose, Saudi Arabia

et al. 2013

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Taif rose (Rosa damascena trigintipetala Dieck) is a sort of damask rose, which is considered as one of the most important economic products of Taif. In this study, the authors investigated the possible cytotoxic, genotoxic, antimutagenic and anticancer effect of concrete and absolute rose oils. The results showed that both concrete and absolute rose oils were cytotoxically and genotoxically safe at a dose of 10 lg/ml when tested on cultures of normal human blood lymphocytes. Also, the results showed significant antimutagenic activity at p \ 0.001 for absolute rose oil at the same dose level when tested on cultures of normal human blood lymphocytes supplemented with 300 ng/ml mitomycin C (MMC). On the other hand, concrete and absolute oils exerted a cytotoxic activity against two kinds of human cancer cell lines: HepG2 and MCF7. Concrete oil showed cytotoxic activity against HepG2 and MCF7 with a half maximal inhibitory concentration (IC 50 ) of 16.28 and 18.09 lg/ml, respectively, whereas absolute rose oil showed its cytotoxic activity against HepG2 and MCF7 with an IC 50 of 24.94 and 19.69, respectively. From this study, it is concluded that concrete and absolute rose oils are cytotoxically and genotoxically safe at a dose of 10 lg/ml when tested on cultures of normal human blood lymphocytes. In addition, absolute oil has an antimutagenic activity at the same dose. Further investigations are needed to study the activity of higher doses of both oils in vitro and in vivo in experimental animals in order to evaluate the capability of using these oils as therapeutic for treatment of some kinds of cancers.

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Sister Chromatid Exchanges, Indices of Human Chromosome Damage and Repair: Detection by Fluorescence and Induction by Mitomycin C

Cited by 381 publications

References 24 publications

Genotixicity of Mitocycin C – Cytogenetic Study

Genotixicity of Mitocycin C – Cytogenetic Study

In vitro genotoxic perspective of Tamiflu

Cytogenetic, cytotoxic and GC–MS studies on concrete and absolute oils from Taif rose, Saudi Arabia

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