Sister-chromatid exchanges: A report of the GENE-TOX program

Antineoplastic Drugs as a Potential Risk Factor in Occupational Settings: Mechanisms of Action at the Cell Level, Genotoxic Effects, and Their Detection Using Different Biomarkers

“…Indirektni su pokazatelj razine oštećenja prisutnih u DNA prije njezina udvostručavanja. Točan mehanizam njihova nastanka nije potpuno razjašnjen (117,118). Kako sam događaj uključuje lomove u četiri lanca DNA (tj.…”

Sister chromatid exchanges induced by two radiosensitizing platinum compounds (cis-dichloro-bis isopropylamine trans dihydroxy platinum IV (CHIP) and cis platinum metronidazole2Cl2(FLAP] in CHO cells in vitro

“…Both solutions were kept in the dark and freshly prepared before each experiment. Experiments were designed according to the protocol suggested by the GENE-TOX Work Group on Sister Chromatid Exchanges (Latt et al, 1981). Asynchronous exponential CHO cell cultures 13-14h after plating 4 x 105 cells per T75 flask were exposed to the drugs for 1 h at 370C.…”

“…Sister chromatid exchange (SCE) formation has been proposed as a sensitive and simple method for detecting the mutagenicity of chemical agents (Latt et al, 1981, Perry, 1980Perry & Evans, 1975;Solomon & Bobrow, 1975;White & Hesketh, 1980).…”

Sister chromatid exchanges in the uridine requiring Chinese hamster cell line, UR-216, cultured in the uridine rich or starved medium.

“…A., Sept. 12, 1983) It is generally recognized that the sister chromatid exchange (SCE) is induced by the treatment with several chemicals giving damage or modification directly to chromosome DNA (Latt et al 1981). According to an experiment that the SCE to be induced by BUdR was suppressed by the administration of deoxycytidine, Davidson et al (1980), however, suggested that the SCE can also be induced by the metabolic perturbation.…”

“…2 Latt et al (1981) most chemicals which can induce SCE give damages directly to DNA. On the other hand, some chemicals such as methotolexate (Banarj ee and Benedict, 1979), 5-fluorodeoxyuridine (Sono and Sakaguchi 1980;Suzuki et al 1982) hydroxyurea (Ishii and Bender, 1980) ; and an excess amount of deoxyribonucleosides (Suzuki and Yosida, 1983) giving no direct effect to DNA can also induce SCE.…”