Sister-chromatid exchange induction by sodium selenite: Dependence on the presence of red blood cells or red blood cell lysate

Ray, James H.; Altenburg, Lewis C.

doi:10.1016/0165-1161(78)90024-9

Cited by 62 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our experiments the test compounds were added to whole blood lymphocyte cultures to exploit the metabolic capability of whole blood compared with isolated lymphocytes (Ray and Altenburg, 1978;Norppa et al, 1980). With no information on the rate of metabolism of zineb and azzurro under these conditions, a long treatment time was chosen leading to a total culture time of up to 72 h. Such a culture time is not optimal Fig.…”

Section: Figmentioning

confidence: 99%

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

Soloneski¹

2001

Mutagenesis

View full text Add to dashboard Cite

The in vitro cytogenetic effects exerted by the dithiocarbamate fungicide zineb and one of its commercial formulations currently used in Argentina, azzurro, were studied in whole blood human lymphocyte cultures. The genotoxicity of the fungicides was measured by analysis of the frequency of chromosomal aberrations and sister chromatid exchanges (SCEs) and cell cycle progression assays. Both zineb and azzurro activities were tested within the range 0.1-100.0 µg/ml immediately after in vitro lymphocyte stimulation. Only concentrations of 50.0 and 100.0 µg/ml zineb and azzurro induced a significant increase in SCE frequency over control values. Furthermore, this genotoxicity appears to be correlated with its cytotoxicity, measured as cell cycle kinetics, since both a significant delay in cell cycle progression and a significant reduction in proliferative rate index were only observed in those cultures treated with these fungicide concentrations. For both chemicals, a progressive dose-related inhibition of the mitotic activity of cultures was observed when increasing the fungicide concentration. Moreover, only the mitotic activity statistically differed from control values when doses of zineb or azzurro <10 µg/ml were employed. For both fungicides the mitotic index reached the minimal value at doses of 100 µg/ml. Both products induced a significant dose-dependent increase in the number of abnormal cells, chromatid-type and chromosome-type aberrations as well as in the total number of aberrations in the 0.1-100.0 µg/ml dose range. Based on these results, the evaluation of zineb as a controversial genotoxic/nongenotoxic compound for human health should be reconsidered. Instead, we demonstrate that the fungicide induces large DNA alterations and should be considered as a clastogenic mutagen.

show abstract

Section: Figmentioning

confidence: 99%

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

Soloneski¹

2001

Mutagenesis

View full text Add to dashboard Cite

show abstract

“…In another study, selenium (VI) compounds induced chromosomal aberrations in cultured human leukocytes whereas selenium (IV) compounds were inactive (164). Selenite did not cause SCE's in purified human lymphocyte cultures or xeroderma pigmentosum cells, however, addition of red blood cell lysate with selenite resulted in induction of SCE in both cell types (165). Induction of chromosomal aberrations and DNA-repair synthesis in human diploid fibroblasts by selenite was enhanced by addition ofa mouse liver S-9 microsomal fraction and NADPH (166).…”

Section: Mutagenicitymentioning

confidence: 89%

The role of metals in carcinogenesis: biochemistry and metabolism.

Jennette¹

1981

Environ Health Perspect

154

View full text Add to dashboard Cite

The oxyanions of vanadium, chromium, molybdenum, arsenic, and selenium are stable forms of these elements in high oxidation states which cross cell membranes using the normal phosphate and/or sulfate transport systems of the cell. Once inside the cell, these oxyanions may sulfuryl transfer reactions. Often the oxyanions serve as alternate enzyme substrates but form ester products which are hydrolytically unstable compared with the sulfate and phosphate esters and, therefore, decompose readily in aqueous solution. Arsenite and selenite are capable of reacting with sulfhydryl groups in proteins. Some cells are able to metabolize redox active oxyanions to forms of the elements in other stable oxidation states. Specific enzymes may be involved in the metabolic processes. The metabolites of these elements may form complexes with small molecules, proteins and nucleic acids which inhibit their ability to function properly. The divalent ions of beryllium, manganese, cobalt, nickel, cadmium, mercury, and lead are stable forms of these elements which may mimic essential divalent ions such as magnesium, calcium, iron, copper, or zinc. These ions may complex small molecules, enzymes, and nucleic acids in such a way that the normal activity of these species is altered. Free radicals may be produced in the presence of these metal ions which damage critical cellular molecules.

show abstract

“…Even when the above-mentioned sources of variation are excluded or minimized, baseline SCE frequencies and cell-cycle progression of the same lymphocytes have been reported to vary in these three culture types from humans [Larramendy and Reigosa, 1986;Larramendy et al, , 1993Larramendy et al, , 1995Larramendy et al, , 1996Ray and Altenburg, 1978], pigs [Larramendy and Reigosa, 1986;Larramendy et al, , 1993Larramendy et al, , 1995Larramendy et al, , 1996Reigosa et al, 1997], and rats [Kligerman et al, 1982;Wilmer et al, 1983Wilmer et al, , 1984.…”

Section: Introductionmentioning

confidence: 93%

Monocytes affect cell‐cycle progression but not baseline frequency of sister chromatid exchanges of pig lymphocytes

Soloneski

Garcı́a

Reigosa

et al. 1999

Environ and Mol Mutagen

View full text Add to dashboard Cite

Sister-chromatid exchange induction by sodium selenite: Dependence on the presence of red blood cells or red blood cell lysate

Cited by 62 publications

References 34 publications

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

Effect of the dithiocarbamate pesticide zineb and its commercial formulation azzurro. I. Genotoxic evaluation on cultured human lymphocytes exposed in vitro

The role of metals in carcinogenesis: biochemistry and metabolism.

Monocytes affect cell‐cycle progression but not baseline frequency of sister chromatid exchanges of pig lymphocytes

Contact Info

Product

Resources

About