Sister chromatid exchange analysis in familial groups of malignant melanoma patients

Illeni, Maria Teresa; Rovini, Dario; Grassi, C; Lombardo, Claudia; Placucci, Marco; Squicciarini, Paolo; Cascinelli, Natale; Ghidoni, A.

doi:10.1016/0165-4608(91)90100-9

Cited by 8 publications

(1 citation statement)

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“…The measurement of SCE in peripheral blood lymphocytes was for many years one of the most popular cytogenetic methods for evaluating human genotoxicity and hereditary disease (6,7,8). It has been reported that Bloom's Syndrome lymphocytes, oral submucous fibrosis, ovarian cancer, malignant mesothelioma, cervical cancers, malignant melanoma, and breast cancer were analyzed through manipulated SCE incidence (9)(10)(11)(12)(13)(14). The aim of this study is to determine whether SCE frequency, as an index of chromosomal instability, is a heritable factor for GIC or not.…”

Section: Introductionmentioning

confidence: 99%

Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives

Turgut

Yaşar²,

Yaykaşlı

et al. 2014

Electron J Gen Med

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Gastrointestinal Cancers (GICs) European Journal of General MedicineOriginal Article Eur J Gen Med 2014; 11(2): 94-98 DOI : 10.15197/sabad.1.11. 46 Eur J Gen Med 2014;11(2): 94-98 Increased sister chromatid exchange in gastrointestinal cancers 95 MATERIALS AND METHODSThis study was conducted at Düzce University Medical Faculty, Department of Medical Genetics, Düzce, Turkey. The study groups consisted of 15 GIC patients (2 esophagus, 6 stomach, 6 colon and 1 rectum), 13 relatives and 15 healthy subjects. Approved ethical certificate was obtained from local ethics committee, and written informed consents of the studied cases were obtained. Age, gender, smoking and alcohol consumption status and tumor markers (alpha-fetoprotein, AFP, carcinoembryonic antigen-CEA, carcinogenic antigen (CA) 125, CA19-9, CA15-3) were recorded. Two milliliters of venous blood was drawn using Na-heparinized syringes from each case. Replication Index was calculated by following formula.RPI: R1 + (R2x2) + (R3x3) / R1 + R2 + R3Mitotic index (MTI) was calculated by following formula. MTI: metaphase/metaphase+lymphocyte count Statistical analysisThe frequency of SCE per metaphase in groups was compared by Kruskal Wallis. Mann-Whitney U test was also used. Relationships between parameters were evaluated with the Spearman's correlation analysis. Qualitative data were compared using Chi-square test. Results with 95% confidence interval and p<0.05 were considered significant.

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