Sirtuin 1 suppresses nuclear factor κB induced transactivation and pro-inflammatory cytokine expression in cat fibroblast cells

Ishikawa, Shingo; Takemitsu, Hiroshi; Habara, Makoto; Mori, Nobuko; Yamamoto, Ichiro; Arai, Toshiro

doi:10.1292/jvms.15-0245

Cited by 5 publications

(4 citation statements)

References 28 publications

(31 reference statements)

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“…Sirtuin 1 (SIRT1), a class III histone/protein deacetylase, is associated with aging and metabolism through maintaining inflammation via NF-kB. The upregulation of NF-kB transcriptional activity and pro-inflammatory cytokine mRNA expression by p65 subunit of NF-kB and lipopolysaccharide were suppressed by SIRT1 [21]. Because of the central role of NF-kB in cytokine-mediated pancreatic β-cell damage, it was postulated that SIRT1 might work in pancreatic β-cell damage models [22].…”

Section: Discussionmentioning

confidence: 99%

Liraglutide exerts an anti-inflammatory action in obese patients with type 2 diabetes

Savchenko

Дігтяр

Selikhova

et al. 2019

Romanian Journal of Internal Medicine

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Introduction. Liraglutide (L) is the analogue of human glucagon-like peptide 1 which stimulates glucose-dependent insulin secretion and can modify the level of inflammatory biomarkers. L can influence NF-kB inflammatory cascade, but the mechanisms of anti-inflammatory activities of L remain to be determined. In animal models L influenced an activity of Sirtuin 1(SIRT1). Moreover, recent evidences strongly suggest that SIRT1 up-regulation may serve as a potent therapeutic approach against development and progression of diabetic complications. The aim of this study was to investigate L effects directed on the pro-inflammatory NF-kB pathway and expression of SIRT1 in obese patients with type 2 diabetes mellitus (DM). Materials and Methods. 15 obese patients with type 2 diabetes were studied, all using metformin (1-2 g/day) and sulfonylurea (glimiperide). All patients received L 1.2 mg daily add-on to stable therapy for 6 weeks. Blood samples were collected before, 6 weeks after start of treatment and after an overnight fast 6 weeks after stopping L, mononuclear cells (MNC) were isolated. The mRNA expressions of TNF-α, TLR2, TLR4, NOD1, IL-2 and SIRT1 were measured in MNC by RT-PCR. Ceruloplasmin concentration was measured in plasma by photometric method. Results. In this add-on pilot clinical investigation we received new data that L can inhibit proinflammatory NF-kB pathway by increased SIRT1 expression in obese patients with type 2 DM improving metabolic profile. The mRNA expression in MNC of TNF-α, IkB, TLR2, TLR4, and plasma ceruloplasmin fell after 6 weeks of L. Expressions of IL-2 and NOD-1 were stable. There was a significant increase of SIRT1 mRNA expression. The mRNA expression in MNC of TNF-α, IkB, TLR2, TLR4, NOD1, SIRT1 and ceruloplasmin concentrations did not reverse to baseline levels after 6 weeks stopping of L treatment. IL-2 expression decreased in comparison with basic level. Conclusions. L has a potent anti-inflammatory effect as do GLP-1 agonists due to inhibition of NF-kB pathways and up-regulate SIRT1 expression, down-regulating pro-inflammatory factors including cytokines (TNF-α), extra- and intracellular receptors (TLR2, TLR4), and inflammation markers such as ceruloplasmin. Long lasting effects of L can be mediated by epigenetic regulation of NF-kB pathway by SIRT-1.

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Section: Discussionmentioning

confidence: 99%

Liraglutide exerts an anti-inflammatory action in obese patients with type 2 diabetes

Savchenko

Дігтяр

Selikhova

et al. 2019

Romanian Journal of Internal Medicine

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“…It is worth nothing that Sirt1 was recently reported that playing critical role to preventing autoimmunity and participate in sepsis progress 55 , 56 . Sirt 1 is a nicotinamide adenine dinucleotide (NAD+) dependent class III histone deacetylase (HDAC) that targets nuclear factor kappa B (NF-kB), a critical transcription factor in the regulation of proinflammatory cytokine production to adapt gene expression to metabolic activity 57 – 59 . Moreover, SIRT1 could regulated immunometabolic polarity during the hyper-inflammatory and hypoinflammatory phases of sepsis 60 .…”

Section: Discussionmentioning

confidence: 99%

Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression

Shao

Zhao

Zhang

et al. 2020

Sci Rep

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Growing evidence indicated that single nucleotide polymorphisms (SNPs) in the apolipoprotein E (APOE) gene are related to increase the risk of many inflammatory-related diseases. However, few genetic studies have associated the APOE gene polymorphism with sepsis. This study was to investigate the clinical relevance of the APOE gene polymorphism in the onset and progression of sepsis. A multicenter case–control association study with a large sample size (601 septic patients and 699 healthy individuals) was conducted. Clinical data showed that the APOEε4 allele was overrepresented among all patients with septic shock (p = 0.031) compared with sepsis subtype, suggesting that APOEε4 allele may associated with increased susceptibility to the progression of sepsis. Moreover, the APOE mRNA levels decreased after lipopolysaccharide (LPS) stimulation in cells in culture. Then 21 healthy individuals to extract PBMC for genotype grouping (APOE4+ group 8; APOE4− group 13) was selected to evaluate the effect on APOE level, and results showed that the expression level of APOE in APOE4+ group and APOE4− group did not differ in mRNA levels after an LPS challenge, but the protein levels in APOE4+ group decreased slower than that in APOE4− group, and this process was accompanied by the upregulation of proinflammatory cytokines. These results provide evidence that the APOEε4 allele might be associated with the development of sepsis and a potential risk factor that can be used in the prognosis of sepsis.

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“…Reactions were carried out with Perfect Real Time TYBR Premix Ex Taq II (Takara, Tokyo, Japan) on an ABI 7300 Real Time PCR Sequence Detection System (Applied Biosystems, Foster City, CA) and the following shuttle PCR protocol: 95°C for 30 s, followed by 40 cycles of 95°C for 5 s, and 60°C for 35 s, in 20 μL reaction volumes containing 2 μL template cDNA, 0.8 μL primers (final concentration: 0.4 μM, including 0.4 μL ROX Reference Dye), and 6.0 μL distilled water. The primers listed in Table 1 were used for sirtuin1 and β-actin mRNA quantitation ( 20 ). Following real-time PCR, the fragment was subjected to dissociation-curve analysis to avoid nonspecific PCR amplification.…”

Section: Methodsmentioning

confidence: 99%

Shiitake mushroom powder supplementation increase antioxidative activity in dogs

Kusaba,

Arai

2024

Front. Vet. Sci.

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IntroductionThe prevalence of age-related diseases, including obesity (a lipid metabolism disorder), increases with the increase in a dog’s lifespan. Most of age-related diseases are associated with oxidative stress by excessive production of reactive oxygen species (ROS) from impaired mitochondrial functions. Safe and effective supplements with antioxidative and anti-inflammatory activities are required to prevent obesity and associated complications. Shiitake mushroom exhibit various functions including antioxidant activity. We investigated the effect of shiitake powder supplementation in healthy dogs.MethodsShiitake powder was supplemented at a dose of 800 mg/kg body weight/day for 4 weeks. The dose was set as 0.60–0.65 mg/kg/day of eritadenine, a hypocholesterolemic factor.ResultsThe body weight and body condition score of the dogs did not change after shiitake supplementation. In contrast, plasma total cholesterol concentrations decreased and superoxide dismutase activity and leukocyte sirtuin1 mRNA expression increased significantly in the dogs that received the supplement.DiscussionOral administration of shiitake powder increased antioxidative activity. The supplement may be useful in ameliorating the signs of age-related diseases, including obesity, in dogs.

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Sirtuin 1 suppresses nuclear factor κB induced transactivation and pro-inflammatory cytokine expression in cat fibroblast cells

Cited by 5 publications

References 28 publications

Liraglutide exerts an anti-inflammatory action in obese patients with type 2 diabetes

Liraglutide exerts an anti-inflammatory action in obese patients with type 2 diabetes

Presence of the apolipoprotein E-ε4 allele is associated with an increased risk of sepsis progression

Shiitake mushroom powder supplementation increase antioxidative activity in dogs

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