Sirt1 protects pig oocyte against <i>in vitro</i> aging

Ma, Rujun; Zhang, Liang; Han, Jun; Ren, Rui

doi:10.1111/asj.12360

Cited by 46 publications

(56 citation statements)

References 38 publications

(41 reference statements)

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“…Sato et al. reported that SIRT1 could improve the quality of porcine oocyte by gaining the copy number of mitochondrial DNA, so that promoted oocyte maturation and embryo development after PA. SIRT1 could protect in vitro porcine oocyte against aging . Similar results were reported by Itami et al.…”

Section: Discussionsupporting

confidence: 88%

Melatonin protects porcine oocyte in vitro maturation from heat stress

Liu

Zhang

et al. 2015

Journal of Pineal Research

111

108

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Melatonin is a pleiotropic molecule which plays an important role in animal reproductive activities. Because of the increased global warming, the impact of heat stress (HS) on stockbreeding has become an inevitable issue to be solved. To investigate the potential effects of melatonin on the in vitro maturation of porcine oocyte under the HS, a HS model for porcine oocyte maturation has been used in this study and the different concentrations of melatonin (10(-6) -10(-9) m) were also tested for their protective effects on oocytes. The polar body rate, the index of the nuclear maturation of the oocytes, and the cleavage rate as well as the blastocyst rate were measured to evaluate the developmental competence of the oocytes after parthenogenetic activation (PA). The results showed that HS [in vitro maturation (IVM) 20-24 hr, 42°C] significantly reduced the polar body rate of oocytes and the blastocyte rate of porcine PA embryos, while melatonin (10(-7) m) application not only improved polar body rate and blastocyte rate, but also preserved the normal levels of steroid hormone which is disrupted by HS. The presence of melatonin (10(-7) m) during the oocyte maturation under the HS reduced reactive oxygen species (ROS) formation, enhanced glutathione (GSH) production, inhibited cell apoptosis, and increased the gene expressions of SIRT1, AKT2, and Polg2. Importantly, the endogenously occurring melatonin of cumulus-oocyte complexes was significantly induced by HS. The results indicated that melatonin application effectively protected the oocytes from HS. These observations warranted the further studies in vivo regarding to improve the reproductive activities of animals under the global warming environment.

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Section: Discussionsupporting

confidence: 88%

Melatonin protects porcine oocyte in vitro maturation from heat stress

Liu

Zhang

et al. 2015

Journal of Pineal Research

111

108

View full text Add to dashboard Cite

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“…Embryos from oocytes aged for more than 48 hr rarely develop into blastocysts (Lee et al, 2014;Miao et al, 2018;Němeček, Dvořáková, Heroutová, Chmelíková, & Sedmíková, 2017). Although aging for 24 hr also decreases oocyte competence, this effect can be recovered by antiaging systems (Lee et al, 2014;Ma, Zhang, Zhang, Han, & Rui, 2015). Accordingly, we selected 24 hr as the in vitro aging period.…”

Section: Discussionmentioning

confidence: 99%

Allicin protects porcine oocytes against damage during aging in vitro

Park

Lee

Yoon

et al. 2019

Molecular Reproduction Devel

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Allicin, a chemical component of garlic, has strong antioxidant activity and is thought to exert antiaging effects in vitro. We investigated whether allicin treatment would protect porcine oocytes and embryos from postovulatory aging mediated by apoptosis and autophagy. The rates of oocyte survival and polar body extrusion in samples treated with 1 µM allicin (1 AL) were significantly higher than in untreated samples (0 AL). In addition, 1 AL prevented defects in spindle formation and chromosome alignment, as well as decreases in the expression of maturation markers, during in vitro aging. In this study, we considered allicin to be a regulator of autophagy rather than an antioxidant or antiapoptotic agent. At the embryo level, although the cleavage rate after parthenogenetic activation was similar in all groups, the blastocyst formation rate was higher in the 1 AL group than in the 0 AL group. Our findings demonstrate that allicin effectively prevents the deterioration of porcine oocytes during aging in vitro, and could therefore be used to improve the quality of aged oocytes used in in vitro experiments.

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“…SIRT1 may protect mitochondria against oxidative stress (Figure ). However, aged oocytes have undetectable SIRT1 expression levels and low ability to regulate SIRT1 . Therefore, aged oocytes may be susceptible to the effects of oxidative stress through their decreased ability to produce SIRT1.…”

Section: Impact On Ovary For Pregnancymentioning

confidence: 99%

Preconception resveratrol intake against infertility: Friend or foe?

Ochiai

Kuroda

2019

Reprod Medicine & Biology

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Background Resveratrol is an antiaging, antioxidant, anti‐inflammatory, and insulin‐sensitizing natural polyphenolic compound. Growing evidence indicates that resveratrol has potential therapeutic effects in infertile women with diminished ovarian function, polycystic ovary syndrome (PCOS), or endometriosis. However, only one clinical trial in women undergoing in vitro fertilization (IVF) cycles using resveratrol has ever been reported. This review focuses on the potential therapeutic effects of resveratrol on pregnancy and on its advantages and disadvantages in pregnancy outcomes during infertility treatment. Methods We performed a literature review to describe the known impacts of resveratrol on the ovary and endometrium. Results Resveratrol upregulates sirtuin (SIRT)1 expression in ovaries, which is associated with protection against oxidative stress. It leads to the activation of telomerase activity and mitochondrial function, improving ovarian function. In the endometrium, resveratrol downregulates the CRABP2‐RAR pathway leading to suppressing decidual and senescent changes of endometrial cells, which is essential for embryo implantation and placentation. Moreover, resveratrol may also induce deacetylation of important decidual‐related genes. Conclusions Resveratrol has potential therapeutic effects for improving ovarian function; however, it also has anti‐deciduogenic actions in uterine endometrium. In addition, its teratogenicity has not yet been ruled out; thus, resveratrol should be avoided during the luteal phase and pregnancy.

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Sirt1 protects pig oocyte against in vitro aging

Cited by 46 publications

References 38 publications

Melatonin protects porcine oocyte in vitro maturation from heat stress

Melatonin protects porcine oocyte in vitro maturation from heat stress

Allicin protects porcine oocytes against damage during aging in vitro

Preconception resveratrol intake against infertility: Friend or foe?

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