SIRT1 knock-in mice preserve ovarian reserve resembling caloric restriction

Long, Guan-Yun; Yang, Jin-Hao; Xu, Jianhui; Ni, Yanhong; Zhou, Xiaoling; Ma, Jiayi; Fu, Yucai; Luo, Lili

doi:10.1016/j.gene.2018.10.040

Cited by 32 publications

(39 citation statements)

References 29 publications

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“…In mice, oocytes undergo a 2–3 week growth phase during which time, follicles develop from primordial to primary, secondary and, finally, antral stages. Since increasing Sirt1 activity sustains a higher follicular pool during ageing (Liu et al, 2013; Long et al, 2019; Zhou et al, 2014), we reasoned that loss of Sirt1 might be detrimental to follicular development. Surprisingly, however, we found no difference in numbers of primordial, primary, secondary or antral follicles between young OoSirt1 ΔEx4/ΔEx4 mice and OoSirt1 +/+ mice (Figure a,b).…”

Section: Resultsmentioning

confidence: 99%

Sirt1 sustains female fertility by slowing age‐related decline in oocyte quality required for post‐fertilization embryo development

2020

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Section: Resultsmentioning

confidence: 99%

Sirt1 sustains female fertility by slowing age‐related decline in oocyte quality required for post‐fertilization embryo development

2020

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“…In addition to the regulation of PKB, FOXO3a is also mediated via acetylation and deacetylation (Kim et al, 2010;Xiang et al, 2012;Zhang et al, 2013;Wang et al, 2014;Zhou et al, 2014;Liu et al, 2015;Long et al, 2019). CREB binding protein, p300, and other nuclear proteins can acetylate lysine on the DNA-binding region of FOXO3a protein, resulting in reduced FOXO3a transcriptional activation (Watroba et al, 2012).…”

Section: Acetylation and Deacetylationmentioning

confidence: 99%

“…CREB binding protein, p300, and other nuclear proteins can acetylate lysine on the DNA-binding region of FOXO3a protein, resulting in reduced FOXO3a transcriptional activation (Watroba et al, 2012). Sirtuin 1 (SIRT1) is a NAD-dependent histone deacetylase (Long et al, 2019). Typically, SIRT1 in the nucleus may activate the transcriptional activity of FOXO3a, regulating cellular functions by deacetylating Ac-FOXO3a (Glauser and Schlegel, 2007).…”

Section: Acetylation and Deacetylationmentioning

confidence: 99%

“…Gorczyca et al (2019) demonstrated the presence of SIRT1 and SIRT6 in ovarian cells, and their involvement in the control of follicular atresia. Recent studies revealed that energy restriction can increase the expression of SIRT1 and activate SIRT1-related signaling pathways in adult mice ( Xiang et al, 2012 ; Zhang et al, 2013 ; Liu et al, 2015 ; Long et al, 2019 ). Additionally, Kim et al (2010) reported that the high expression of FOXO3a can upregulate SITR6 activity, whereas the inhibition of FOXO3a expression could downregulate SIRT6 activity.…”

Section: Expression Regulation Of Foxo3a During Follicular Developmenmentioning

confidence: 99%

“…However, PI3K-PKB-FOXO3 can promote autophagy by mediating mTOR inhibition ( van der Vos et al, 2012 ). Long et al (2019) reported that oocyte-specific SIRT1-overexpressing mice demonstrated an improved follicle reserve and a prolonged ovarian lifespan by continuously activating FOXO3a and suppressing mTOR. Furthermore, SIRT1 can facilitate primordial follicle recruitment through directly modulating PKB and mTOR transcription, independent of deacetylase activity ( Zhang T. et al, 2019 ).…”

Section: Physiological Roles Of Foxo3a During Follicular Developmentmentioning

confidence: 99%

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Expression Regulation and Physiological Role of Transcription Factor FOXO3a During Ovarian Follicular Development

et al. 2020

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In mammals, developing ovarian follicles transform from primordial follicles to primary follicles, secondary follicles, and mature follicles, accompanied by changes in follicular secretory functions. FoxO3a is a member of the forkhead transcription factor family (FoxO), which plays an important role in the cell cycle, DNA damage repair, apoptosis, oxidative stress, and energy metabolism. Recent studies have shown that FOXO3a is involved in the physiological regulation of follicular development and pathological progression of related ovarian diseases, which will provide useful concepts and strategies for retarding ovarian aging, prolonging the ovarian life span, and treating ovarian diseases. Therefore, the regulation of FOXO3a expression, as well as the physiological contribution during ovarian follicular development are detailed in this paper, presenting an important reference for the further study of ovarian biology.

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Expression of sirtuins in ovarian follicles of postnatal mice

Kong

Yao

Bai

et al. 2020

Molecular Reproduction Devel

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Mammalian ovarian follicular development is an intricate, elaborate, and wellorganized phenomenon regulated by various signaling pathways; however, the underlying mechanism remains unclear. Mammalian sirtuins (sirtuin 1 to sirtuin 7) are a group of NAD +-dependent deacetylases implicated in various physiological processes including cell proliferation, apoptosis, cell cycle progression, and insulin signaling. Mammalian ovarian sirtuins have been studied using adult and aged bovine, porcine, and murine models. However, limited information is available regarding their precise expression patterns and the localization of follicle development in mice. This study aimed to assess the dynamic expression and localization of all seven sirtuins in early postnatal mouse ovaries through real-time polymerase chain reaction analysis and immunohistochemistry, respectively. During postnatal ovarian follicle development, sirtuin 1, sirtuin 4, and sirtuin 6 were downregulated compared with those in 1-day postnatal mouse ovaries (p < .05), indicating that these three sirtuin genes may be markers of follicular development. Combining their localization in granulosa cells through immunohistochemical studies, sirtuin 1, sirtuin 4, and sirtuin 6 are suggested to play negative regulatory roles in mammal ovarian follicular granulosa cell development. Furthermore, we found that sirtuin 2 (p < .05) and sirtuin 7 (p < .05) mRNA were constantly upregulated relative to sirtuin 1, although limited information is available regarding sirtuin 7. Among all sirtuins in mouse ovaries, sirtuin 1 was relatively and steadily downregulated. Upon sirtuin 1 overexpression in 1-day postnatal mouse ovaries via sirtuin 1-harboring adenoviruses in vitro, the emergence of primary follicles was delayed, as was the emergence of secondary follicles in 4-day postnatal ovaries. Further studies on KGN cell lines reported that interfering with sirtuin 1 expression in granulosa cell significantly affected granulosa cell proliferation and the expression of mitochondrial genes. This study presents the first systemic analysis of dynamic patterns of sirtuin family expression in early postnatal mice ovaries, laying the foundation for further studies on less discussed sirtuin subtypes, such as sirtuin 5 and sirtuin 7.

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SIRT1 knock-in mice preserve ovarian reserve resembling caloric restriction

Cited by 32 publications

References 29 publications

Sirt1 sustains female fertility by slowing age‐related decline in oocyte quality required for post‐fertilization embryo development

Sirt1 sustains female fertility by slowing age‐related decline in oocyte quality required for post‐fertilization embryo development

Expression Regulation and Physiological Role of Transcription Factor FOXO3a During Ovarian Follicular Development

Expression of sirtuins in ovarian follicles of postnatal mice

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