siRNAs Targeting Viral Protein 5: The Major Capsid Protein of Herpes Simplex Virus-1 Affects its Propagation and Cytoskeleton

Ma, Kaiqi; Jin, Fujun; Wang, Qiaoli; Ren, Zhe; Zheng, Kai; Wang, Yifei

doi:10.4314/tjpr.v14i3.6

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ICP5 is a major structural protein which acts as a scaffold during the formation of HSV's capsid and plays an important role in virus packaging and maturity. This protein is necessary for HSV-1 proliferation [33]. Finally, gB is an envelope glycoprotein required for HSV fusion [34].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

Gonzalez,

Vizoso-Pinto,

Erra-Balsells

et al. 2024

IJMS

View full text Add to dashboard Cite

Herpes simplex virus (HSV) infections are highly widespread among humans, producing symptoms ranging from ulcerative lesions to severe diseases such as blindness and life-threatening encephalitis. At present, there are no vaccines available, and some existing antiviral treatments can be ineffective or lead to adverse effects. As a result, there is a need for new anti-HSV drugs. In this report, the in vitro anti-HSV effect of 9,9′-norharmane dimer (nHo-dimer), which belongs to the β-carboline (βC) alkaloid family, was evaluated. The dimer exhibited no virucidal properties and did not impede either the attachment or penetration steps of viral particles. The antiviral effect was only exerted under the constant presence of the dimer in the incubation media, and the mechanism of action was found to involve later events of virus infection. Analysis of fluorescence lifetime imaging data showed that the nHo-dimer internalized well into the cells when present in the extracellular incubation medium, with a preferential accumulation into perinuclear organelles including mitochondria. After washing the host cells with fresh medium free of nHo-dimer, the signal decreased, suggesting the partial release of the compound from the cells. This agrees with the observation that the antiviral effect is solely manifested when the alkaloid is consistently present in the incubation media.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

Gonzalez,

Vizoso-Pinto,

Erra-Balsells

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…It has shown that the synthesized ICP4-targeting siRNA exhibits excellent antiviral activity in inhibiting HSV-1 replication in retinal pigment epithelial cells (18). Also, siRNAs targeting VP5 (HSV-1 major capsid protein) confers excellent antiviral activity by inhibiting HSV-1 replication in cells (24). Because the effect of the novel miR-SX1 on HSV-1 has not yet been investigated, we focused to study the effect of miR-SX1 mimics on HSV-1 replication in HeLa cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro

et al. 2019

View full text Add to dashboard Cite

Herpes simplex virus type 1 (HSV-1) infections are one of the most common diseases in human population. HSV-1 causes subclinical, mild to severe diseases, especially in immunocompromised patients. Acyclovir has been used to reduce manifestations of HSV-1 infections. The extensive use of this drug has led to the development of resistant strains. Thus, designing a novel anti-herpes drug with different mechanisms of action is urgently needed. Cellular microRNAs (miRNAs) have direct antiviral effects in addition to their regulatory functions. In this study we used a novel miRNA (hsa-miR-7704), expressed in macrophages, to inhibit HSV-1 lytic infection in HeLa cells. Synthesized hsa-miR-7704 mimics were transfected into HSV-1 infected HeLa cell. The inhibitory effects of the miRNA were evaluated by plaque assay, real time polymerase chain reaction and the viral titers were measured by the 50% tissue culture infective dose (TCID50). The viral titer and cell cytopathic effect were dramatically decreased in HeLa cells transfected with hsa-miR-7704 (50 and 100 nM), compared with HSV-1 infected cells alone or transfected with the mock miRNA control. These results suggest that hsa-miR-7704 inhibits HSV-1 replication efficiently in vitro. This may provide an alternative mechanism to prevent HSV-1 infections.

show abstract

siRNAs Targeting Viral Protein 5: The Major Capsid Protein of Herpes Simplex Virus-1 Affects its Propagation and Cytoskeleton

Cited by 2 publications

References 19 publications

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

Inhibition of herpes simplex virus type 1 replication by novel hsa-miR-7704 in vitro

Contact Info

Product

Resources

About