SiRNA-mediated silencing of <i>Snail-1</i> induces apoptosis and alters micro RNA expression in human urinary bladder cancer cell line

Shenas, Seyed Mohammad Hossein Musavi; Mansoori, Behzad; Mohammadi, Ali; Salehi, Shima; Kaffash, Behzad; Talebi, Behnaz; Babaloo, Zohreh; Shanehbandi, Dariush; Baradaran, Behzad

doi:10.1080/21691401.2016.1198361

Cited by 19 publications

(9 citation statements)

References 20 publications

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“…Strong evidence exists to support that miRNAs can act as powerful tumor suppressors or oncogenes. Accordingly, they are aberrantly expressed in different types of cancer, in which they suppress apoptosis or promote angiogenesis, tumor growth or metastatic processes …”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‐146a promotes cell migration in Helicobacter pylori–negative gastric cancer

Shomali

Shirafkan

Duijf

et al. 2018

J of Cellular Biochemistry

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microRNAs (miRs) are short noncoding RNAs that post-transcriptionally suppress gene expression. miR-146a acts as an oncogene or a tumor suppressor in various cancers, including gastric cancer, but it is unclear what determines whether miR-146a is oncogenic or tumor suppressive and the molecular mechanisms are still largely unknown. The aim of this study was to investigate the role of miR-146a in gastric cancer, by focusing on its expression in patients who were negative for Helicobacter pylori and its reduced and increased expression effect in vitro. Twenty gastric cancer patients who were negative for H. pylori infection were selected and the expression levels of miRNA-146a in these gastric tumors, in their matched normal gastric tissues and in gastric cancer cell lines with varying tumorigenic potential was measured. Further, the impact of increased and decreased miR-146a expression levels on the expression of predicted target genes, cell migration, viability, proliferation, and apoptosis was examined, respectively. Our results for the first time indicated that miR-146a is downregulated in H. pylori-negative gastric cancers and suggests that H. pylori infection determines whether miR-146a acts as an oncogene or tumor suppressor. The level of miR-146a expression inversely correlates with the tumorigenicity of three gastric cancer cell lines and low miR-146a expression predicts poor recurrence-free survival. It was also found that miR-146a reduces the expression levels of the prometastatic genes and suppresses MKN-45 cell migration. Functional studies showed that miR-146a acts as a tumor suppressor miR and identifies miR-146a as a candidate for antimetastatic miRNA replacement therapy for gastric cancer patients. K E Y W O R D Sapoptosis, cell viability, gastric cancer, Helicobacter pylori-negative patients, metastasis, miR-146a

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Section: Introductionmentioning

confidence: 99%

Downregulation of miR‐146a promotes cell migration in Helicobacter pylori–negative gastric cancer

Shomali

Shirafkan

Duijf

et al. 2018

J of Cellular Biochemistry

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“…Decreased level of MMP‐9 protein as a consequence of miR‐143 overexpression has also inhibitory impacts on cell migration and invasion in pancreatic cancer (Hu, Ou, Wu, Chen, & Sun, ). Involvement of miR‐143 in epithelial–mesenchymal transition (EMT) has also shown in metastatic cancer cells (Musavi Shenas et al, ). Huang et al () demonstrated that, miR‐143 and miR‐145 could prohibit the bone metastasis of prostate cancer by restraining cancer stem cell markers and stemness factors including CD133 , c‐MYC , KLF‐4 , OCT‐4 , and CD44 .…”

Section: Discussionmentioning

confidence: 99%

miRNA‐143 replacement therapy harnesses the proliferation and migration of colorectal cancer cells in vitro

Karimi

Zeinali

Hosseinahli

et al. 2019

Journal Cellular Physiology

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miR-143 is a tumor suppressor miRNA which its downregulation is frequently reported in colorectal cancer (CRC). This miRNA is a negative regulator of K-RAS, c-MYC, BCL-2, and MMP-9 genes which are engaged in tumor growth and metastasis.In the present study, miR-143 restoration was performed by transfection of the pCMV-miR-143 vector into the SW-480 CRC cells. Subsequently, alterations in proliferative and migratory potential of the cells were investigated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and wound-healing assays, respectively. Moreover, to detect apoptosis incidence in the transfected cells, 4',6-diamidino-2-phenylindole (DAPI) staining was used. Furthermore, mRNA levels of c-MYC, K-RAS, MMP-9, and BCL-2, as potential targets of miR-143, were assessed by quantitative Real-Time PCR (qRT-PCR). Also the expression levels of c-MYC, K-RAS, and MMP-9 proteins were investigated by the western blot analysis. Finally, the ratio of BAX to BCL-2 expression, as a potential marker of the response to apoptosis stimuli, was compared between the control and test groups. Furthermore, the trypan blue test was performed to determine the cell viability in cell suspension. According to the results, a decreased viability and migratory potential was observed for the miR-143 receiving cells.The DAPI staining also confirmed the occurrence of apoptosis. Moreover, BCL-2, K-RAS, MMP-9, and c-MYC mRNAs were significantly downregulated in the miR-143 grafted cells.The BAX/BCL-2 ratio also indicated a notable increase in the cells with miR-143 overexpression. In brief, miR-143 replacement could be considered as an effective strategy for the management of CRC and attenuating its invasive features.

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“…Since elevated level of miR-21 in cancer secreted microvesicles induced myoblast apoptosis, leading to cachexia through TLR-7, Nakamura et al [2016] opined that blocking the binding between TLR-7/8 and miR-21 or inhibiting the secretion of microvesicles by suppressing fusion between microvesicles and muscle cells might prove to be a potential therapeutic target for cancer induced cachexia ( 107 ). In BC, SNAIL1 regulated by Akt/GSK/3β-pathway, is reported to promote miR-21 expression transcriptionally ( 108 ). Additionally, the generation and upregulation of miR-21 are reported to be promoted by environmental stimuli such as UV-radiations and smoking, enforcing downregulation of its TS-targets and ultimately causing melanomagenesis ( 109 ).…”

Section: Micrornas In Cancermentioning

confidence: 99%

MicroRNAs: potential biomarkers for diagnosis and prognosis of different cancers

Sharma¹,

Gupta²

2020

Transl Cancer Res TCR

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A thorough understanding of the tumor environment and underlying genetic factors helps in the better formulation of cancer management strategies. Availability of efficient diagnostic and prognostic biomarkers facilitates early detection and progression of the disease. MicroRNAs affect different biological processes participating in tumorigenesis through regulation of their target genes. An expanding list of unique RNAs and understanding of their regulatory role has opened up a new field in cancer research.Based on a comprehensive literature search, we identified 728 miRNAs dysregulated in sixteen cancer types namely bladder cancer (BC), breast cancer (BrC), cervical cancer (CC), colorectal cancer (CRC), esophageal cancer (EC), endometrial cancer (EnC), gastric cancer (GC), hepatocellular cancer (HCC), head and neck squamous cell cancer (HNSCC), lung cancer (LC), ovarian cancer (OC), pancreatic cancer (PC), prostate cancer (PrC), renal cell cancer (RCC), skin cancer (SC), and thyroid cancer (TC). Expression of 43 miRNAs was either upregulated or downregulated in six or more of these cancers. Finally, seven miRNAs namely mir-18a, mir-21, mir-143/145, mir-210, mir-218, mir-221, showing maximum dysregulation, either up-or down-regulation in the majority of cancers, were selected for a detailed presentation of their expression and evaluation of their potential as biomarkers in the diagnosis and prognosis of different cancers.

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SiRNA-mediated silencing of Snail-1 induces apoptosis and alters micro RNA expression in human urinary bladder cancer cell line

Cited by 19 publications

References 20 publications

Downregulation of miR‐146a promotes cell migration in Helicobacter pylori–negative gastric cancer

Downregulation of miR‐146a promotes cell migration in Helicobacter pylori–negative gastric cancer

miRNA‐143 replacement therapy harnesses the proliferation and migration of colorectal cancer cells in vitro

MicroRNAs: potential biomarkers for diagnosis and prognosis of different cancers

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