siRNA Delivery Mediated by Copolymer Nanoparticles, Phospholipid Stabilized Sulphur Hexafluoride Microbubbles and Ultrasound

Shi, Qiusheng; Liu, Peifeng; Sun, Ying; Zhang, Huiping; Du, Jing; Fan, Li; Du, Lianfang; Duan, Yourong

doi:10.1166/jbn.2014.1728

Cited by 20 publications

(14 citation statements)

References 22 publications

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“…Microbubbles can release its payload at specific position, in which the drug vehicle receives US energy. [4][5][6][7] The interactions between microbubbles and US can increase the permeability of particularly in terms of high drug-loading, sustained release action, and targeted lymphatic absorption. 15 16 The potential advantages of both microbubbles and nanoparticles motivated this research to develop a new drug delivery vehicle.…”

Section: Introductionmentioning

confidence: 99%

A Novel Microbubble Capable of Ultrasound-Triggered Release of Drug-Loaded Nanoparticles

Wang¹,

Li²,

Tian³

et al. 2016

j biomed nanotechnol

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Drug-loaded microbubbles have shown attractive potential in disease treatment applications. The present work presents a unique ultrasound (US)-triggered system in which drug-loaded nanoparticles and perfluorocarbon gas are encapsulated within the internal space of microbubbles. The prepared curcumin-loaded albumin nanoparticle payload microbubbles (CcmANP-MB) exhibited a mean diameter of 4895.1 nm ± 421.2 nm and a drug-loading efficiency of 2.23% ± 0.08% (297% increase compared with the drug loading of common drug-loaded microbubbles). US allowed the release of the internal payload. In vitro US-triggered drug release experiments showed that the drug release of CcmANP-MB was delayed by lipid membranes and significantly increased after sonication. In vitro and in vivo US imaging experiments demonstrated that CcmANP-MB evidently enhances US imaging, which indicates that the microbubbles possess good acoustic properties even after encapsulation of nanoparticles. Tumor bearing mice were administered with CcmANP-MB through the tail vein and were then exposed to ultrasound, which resulted in an enhanced drug accumulation in tumor tissues and a significant increase in tumor growth inhibition rate (57.1%) compared with CcmANP-MB alone (28.8%) as well as curcumin-loaded albumin nanoparticle (26.2%). Therefore, the combination of lecithin microbubbles and albumin nanoparticles provides a platform for targeted drug delivery in clinical therapy and disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

A Novel Microbubble Capable of Ultrasound-Triggered Release of Drug-Loaded Nanoparticles

Wang¹,

Li²,

Tian³

et al. 2016

j biomed nanotechnol

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“…After comprehensive analysis, the optimization parameters for the DA-PLGA-PEG-cRGD NPs combined with US were selected as follows: a DA concentration of 20 µg/mL, 30% duty cycle, an ultrasonic time of 90 s, and an ultrasonic intensity of 1.0 W [41]. …”

Section: Resultsmentioning

confidence: 99%

Prevention of Oxidized Low Density Lipoprotein-Induced Endothelial Cell Injury by DA-PLGA-PEG-cRGD Nanoparticles Combined with Ultrasound

Huang

et al. 2017

IJMS

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In general, atherosclerosis is considered to be a form of chronic inflammation. Dexamethasone has anti-inflammatory effects in atherosclerosis, but it was not considered for long-term administration on account of a poor pharmacokinetic profile and adverse side effects. Nanoparticles in which drugs can be dissolved, encapsulated, entrapped or chemically attached to the particle surface have abilities to incorporate dexamethasone and to be used as controlled or targeted drug delivery system. Long circulatory polymeric nanoparticles present as an assisting approach for controlled and targeted release of the encapsulated drug at the atherosclerotic site. Polymeric nanoparticles combined with ultrasound (US) are widely applied in cancer treatment due to their time applications, low cost, simplicity, and safety. However, there are few studies on atherosclerosis treatment using polymeric nanoparticles combined with US. In this study, targeted dexamethasone acetate (DA)-loaded poly (lactide-glycolide)-polyethylene glycol-cRGD (PLGA-PEG-cRGD) nanoparticles (DA-PLGA-PEG-cRGD NPs) were prepared by the emulsion-evaporation method using cRGD modified PLGA-PEG polymeric materials (PLGA-PEG-cRGD) prepared as the carrier. The average particle size of DA-PLGA-PEG-cRGD NPs was 221.6 ± 0.9 nm. Morphology of the nanoparticles was spherical and uniformly dispersed. In addition, the DA released profiles suggested that ultrasound could promote drug release from the nanocarriers and accelerate the rate of release. In vitro, the cellular uptake process of fluorescein isothiocyanate (FITC)@DA-PLGA-PEG-cRGD NPs combined with US into the damaged human umbilical vein endothelial cells (HUVECs) indicated that US promoted rapid intracellular uptake of FITC@DA- PLGA-PEG-cRGD NPs. The cell viability of DA-PLGA-PEG-cRGD NPs combined with US reached 91.9% ± 0.2%, which demonstrated that DA-PLGA-PEG-cRGD NPs combined with US had a positive therapeutic effect on damaged HUVECs. Overall, DA-PLGA-PEG-cRGD NPs in combination with US may provide a promising drug delivery system to enhance the therapeutic effects of these chemotherapeutics at the cellular level.

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“…29 Then, with the degradation of PEAL, the siRNA entrapped in the NPs was gradually released and exhibited sustained release patterns over an extended period. We all know that siRNA is greatly constricted by its instability and quick degradation in the body.…”

Section: Preparation and Characterization Of Sirna-loaded Peal Npsmentioning

confidence: 99%

Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro

Teng¹,

Bai²,

Sun³

et al. 2015

IJN

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The gene knockdown activity of small interfering RNA (siRNA) has led to their use as potential therapeutics for a variety of diseases. However, successful gene therapy requires safe and efficient delivery systems. In this study, we choose mPEG-PLGA-PLL nanoparticles (PEAL NPs) with ultrasound targeted microbubble destruction (UTMD) to efficiently deliver siRNA into cells. An emulsification-solvent evaporation method was used to prepare siRNA-loaded PEAL NPs. The NPs possessed an average size of 132.6±10.3 nm (n=5), with a uniform spherical shape, and had an encapsulation efficiency (EE) of more than 98%. As demonstrated by MTT assay, neither PEAL NPs nor siRNA-loaded PEAL NPs showed cytotoxicity even at high concentrations. The results of cellular uptake showed, with the assistance of UTMD, the siRNA-loaded PEAL NPs can be effectively internalized and can subsequently release siRNA in cells. Taken together, PEAL NPs with UTMD may be highly promising for siRNA delivery, making it possible to fully exploit the potential of siRNA-based therapeutics.

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siRNA Delivery Mediated by Copolymer Nanoparticles, Phospholipid Stabilized Sulphur Hexafluoride Microbubbles and Ultrasound

Cited by 20 publications

References 22 publications

A Novel Microbubble Capable of Ultrasound-Triggered Release of Drug-Loaded Nanoparticles

A Novel Microbubble Capable of Ultrasound-Triggered Release of Drug-Loaded Nanoparticles

Prevention of Oxidized Low Density Lipoprotein-Induced Endothelial Cell Injury by DA-PLGA-PEG-cRGD Nanoparticles Combined with Ultrasound

Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro

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