SIPNs polymeric scaffold for use in cartilaginous tissue engineering: physical-chemical evaluation and biological behavior

Dias, Elcio Malcher; Dias, Dayane dos Reis Costa; Rodrigues, Ana Paula; Dias, Carmen Gilda Barroso Tavares; Bastos, Gilmara de Nazareth Tavares; Oliveira, Johnatt Allan Rocha de; Filho, Rubens Maciel; Passos, Marcele Fonseca

doi:10.1016/j.mtcomm.2021.102111

Cited by 2 publications

(1 citation statement)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, hydrogel—a highly hydrated polymeric crosslinked three-dimensional (3D) network—has attracted ample interest as a scaffolding material due to some of its exceptional properties such as high-water content, stability, flexibility, biocompatibility, degradability, similarity to natural tissue, tunability in physical, chemical and mechanical properties, as well as porosity required to impart a chondrosupportive and/or chondroinductive milieu [ 8 ]. Over the past few decades, semi-IPN networks of hydrogels were widely used for cartilage tissue engineering [ 9 , 10 , 11 , 12 ]. Its advantages include ease fabrication and marked mechanical and thermal properties, among others.…”

Section: Introductionmentioning

confidence: 99%

Degradation-Dependent Stress Relaxing Semi-Interpenetrating Networks of Hydroxyethyl Cellulose in Gelatin-PEG Hydrogel with Good Mechanical Stability and Reversibility

Dey

Agnelli

Borsani

et al. 2021

Gels

View full text Add to dashboard Cite

The mechanical milieu of the extracellular matrix (ECM) plays a key role in modulating the cellular responses. The native ECM exhibits viscoelasticity with stress relaxation behavior. Here, we reported the preparation of degradation-mediated stress relaxing semi-interpenetrating (semi-IPN) polymeric networks of hydroxyethyl cellulose in the crosslinked gelatin-polyethylene glycol (PEG) architecture, leveraging a newly developed synthesis protocol which successively includes one-pot gelation under physiological conditions, freeze-drying and a post-curing process. Fourier transform infrared (FTIR) confirmed the formation of the semi-IPN blend mixture. A surface morphology analysis revealed an open pore porous structure with a compact skin on the surface. The hydrogel showed a high water-absorption ability (720.00 ± 32.0%) indicating the ability of retaining a hydrophilic nature even after covalent crosslinking with functionalized PEG. Detailed mechanical properties such as tensile, compressive, cyclic compression and stress relaxation tests were conducted at different intervals over 28 days of hydrolytic degradation. Overall, the collective mechanical properties of the hydrogel resembled the mechanics of cartilage tissue. The rate of stress relaxation gradually increased with an increasing swelling ratio. Hydrolytic degradation led to a marked increase in the percentage dissipation energy and stress relaxation response, indicating the degradation-dependent viscoelasticity of the hydrogel. Strikingly, the hydrogel maintained the structural stability even after degrading two-thirds of its initial mass after a month-long hydrolytic degradation. This study demonstrates that this semi-IPN G-PEG-HEC hydrogel possesses bright prospects as a potential scaffolding material in tissue engineering.

show abstract