SiO2/Nb2O5 sol–gel as a support for HRP immobilization in biosensor preparation for phenol detection

Rosatto, Simone S.; Sotomayor, Marı́a Del Pilar Taboada; Kubota, Lauro T.; Gushikem, Yoshitaka

doi:10.1016/s0013-4686(02)00516-9

Cited by 68 publications

(45 citation statements)

References 53 publications

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“…28,29 Therefore, the electron-transfer process between the protein and the electrode could be promoted by an electron-hopping mechanism. 30 Figure 4 shows cyclic voltammograms of the HRP/ATP/GC electrode in 0.1 M pH 6.0 PBS in both the presence and the absence of H2O2. When H2O2 was added, compared with the system with no H2O2 present, the cathodic peak current of the electrode increased dramatically, and the anodic peak almost disappeared.…”

Section: Afm Characterization Of the Biosensor Constructionmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalytic Behavior of Horseradish Peroxidase on Attapulgite Clay Modified Electrode

et al. 2011

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Section: Afm Characterization Of the Biosensor Constructionmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalytic Behavior of Horseradish Peroxidase on Attapulgite Clay Modified Electrode

et al. 2011

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“…Many different methods such as covalent immobilization (Imabayashi et al, 2001), sol-gel derived matrix (Rosatto et al, 2002), recently LbL assembly was employed for modification of electrodes (S. Yang et al, 2006). And still the combination of oxidoreductases and amperometric electrodes is the most commonly studied biosensor concept (Fig.…”

Section: Horseradish Peroxidase Electrodes For Phenolic Compounds Detmentioning

confidence: 99%

Hybrid Film Biosensor for Phenolic Compounds Detection

Cabaj¹,

Jadwiga²

2011

Environmental Biosensors

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“…Among several methods of analyses, biosensors are promising tools for detecting phenol and its derivatives due to their simplicity, high sensitivity, ease of use, and suitability for on-site monitoring [2]. Horseradish peroxidase (HRP) is a commonly used enzyme for phenol detection due to its high sensitivity towards a great number of phenolic compounds [3].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Gold Nanoparticles/Polypyrrole/HRP Electrode for Phenol Biosensor by Electropolymerization

Kumpangpet¹,

Jongsomjit²,

Thanachayanont³

et al. 2012

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Abstract.A phenol enzyme biosensor was fabricated in this research using disposable screen-printed carbon electrode modified with gold nanoparticles (AuNPs), polypyrrole (PPy) and immobilized horseradish peroxidase (HRP) by electropolymerization method. The physical and chemical properties of the modified electrode was characterized by scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDX) while electrochemical analysis was performed with amperometry. The fabrication parameters, gold precursor and enzyme concentrations, were optimized. The obtained results proved that AuNPs/PPy nanocomposite matrix could not only appropriately immobilize HRP, but also retained its bioactivity. Furthermore, the presence of AuNPs provided enhanced electrochemical responses. The proposed method was simple, and effective.

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SiO2/Nb2O5 sol–gel as a support for HRP immobilization in biosensor preparation for phenol detection

Cited by 68 publications

References 53 publications

Direct Electrochemistry and Electrocatalytic Behavior of Horseradish Peroxidase on Attapulgite Clay Modified Electrode

Direct Electrochemistry and Electrocatalytic Behavior of Horseradish Peroxidase on Attapulgite Clay Modified Electrode

Hybrid Film Biosensor for Phenolic Compounds Detection

Fabrication of Gold Nanoparticles/Polypyrrole/HRP Electrode for Phenol Biosensor by Electropolymerization

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