Sinusoidal voltammetry:

Brazill, Sara A.; Bender, Sharin; Hebert, Nicole; Cullison, John K.; Kristensen, Eric W.; Kuhr, Werner G.

doi:10.1016/s0022-0728(02)01067-7

Cited by 31 publications

(38 citation statements)

References 45 publications

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“…As mentioned earlier in this work, the frequency domain is the spectral fingerprint for a particular electrochemically active molecule [41]. The current magnitude is proportional to the concentration of the analyte, however, the phase angle information is independent of concentration and consistent within a given set of experimental conditions.…”

Section: Consistency In Frequency Domainmentioning

confidence: 82%

“…The SV detection system has been described in great detail in previous publications from our group [40][41][42].…”

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

“…In previous work, the dependence of the frequency domain "fingerprint" response on the electrochemical characteristics of the analyte and experimental parameters chosen has been discussed [40][41][42]. The three-dimensional frequency response for the analyte of interest was obtained from the scan in time where the background subtracted signal was at its maximum (i.e., the top of the analyte peak).…”

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

“…This was merely the current magnitude of the scans collected throughout the duration of the experiment. The digital equivalent of a lock-in detector was thoroughly described in previous work [41]. The optimum phase angle for the analyte of interest, obtained from the frequency domain, was used to monitor the raw signal.…”

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

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Use of an electrochemically labeled nucleotide terminator for known point mutation analysis

Brazill

Hebert

Kuhr³

2003

Electrophoresis

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A novel single nucleotide polymorphism (SNP) assay utilizing an electrochemically tagged chain terminator is described. The system employs the single-base extension (SBE) technique coupled to capillary gel electrophoresis with end-column electrochemical detection. A redox-labeled chain terminator, ferrocene-acycloATP, is used in the SBE reaction. When the mutation site corresponds to the labeled chain terminator, the extension product is rendered electroactive. The reaction mixture is subsequently separated by capillary gel electrophoresis and the extension product detected at the separation anode with sinusoidal voltammetry. This work demonstrates the first known SNP assay utilizing redox-active chain terminators coupled to electrochemical detection. The methodology presented could lead to a fast, simple, and cost-effective SNP scoring system.

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Section: Consistency In Frequency Domainmentioning

confidence: 82%

“…The SV detection system has been described in great detail in previous publications from our group [40][41][42].…”

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

Section: Sinusoidal Voltammetrymentioning

confidence: 99%

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Use of an electrochemically labeled nucleotide terminator for known point mutation analysis

Brazill

Hebert

Kuhr³

2003

Electrophoresis

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“…In these devices the electrode usually has at least one dimension on the millimeter or larger length scale, but the use of microelectrodes as EC detectors is also attracting attention [1,11]. In addition to the features of EC detectors already cited, the low currents passed minimize resistive effects, so allowing use without supporting electrolyte [12] and in quite resistive media.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Assessment of the Microjet Electrode as a Detector for HPLC

Cannan

Unwin

2004

Electroanalysis

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The microjet electrode (MJE) is characterized as a detector for high performance liquid chromatography (HPLC). Voltammetric measurements of the oxidation of hydroquinone (HQ) allow mass transport to be determined for the MJE detector configuration, and the factors controlling the conversion efficiency of the device to be well understood. The current-time response to the flow injection analysis of volumes of solution in the 10 ± 80 mL range has been established, and the limit of detection of this method has been determined. The latter was found to approach that of UV absorbance measurements, which is particularly encouraging, given that HQ has a relatively strong chromophore (e 2 290.8 cm À1 mol À1 L). This detection system is a robust and simple arrangement with the capability of analyzing large volumes of eluent at typical analytical HPLC flow rates.

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