Sinus aspirates in chronic rhinosinusitis: fungal colonization of paranasal sinuses, evaluation of ICAM-1 and IL-8 and studying of immunological effect of long-term macrolide therapy

Elmorsy, Shawky; El-Naggar, M.M.; aal, Sanaa M Abdel; Abou-elela, Mohammad A

doi:10.4193/rhin09.140

Cited by 10 publications

(8 citation statements)

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“…The treatment is thought to work via an anti-inflammatory mechanism, not by antibacterial activity. EM reduced the levels of IL-8 and ICAM-1 in patients with chronic rhinosinusitis; this finding indicates that EM can modulate the immune status of these patients [8] .…”

Section: Discussionmentioning

confidence: 67%

Effects of a Novel Nonantibiotic Macrolide, EM900, on Cytokine and Mucin Gene Expression in a Human Airway Epithelial Cell Line

et al. 2011

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Background/Aims: Long-term macrolide therapy is an effective treatment for chronic sinusitis and diffuse panbronchiolitis. However, long-term use of macrolides may promote the growth of drug-resistant bacteria; therefore, development of macrolides with no antibacterial action is desirable. A new erythromycin (EM) derivative, (8R,9S)- 8,9-dihydro-6,9-epoxy-8,9-anhydropseudoerythromycin A (EM900), does not possess antibacterial action. Methods: To determine whether EM900 induced a clinically relevant anti-inflammatory response and repressed mucin gene expression in cells derived from human airway epithelia, we assessed the effects of EM900 on IL-1β-induced inflammatory cytokines in A549 cells and MUC5AC gene expression in HM3-MUC5AC cells. We also investigated the effects of EM900 on IL-1β-induced NF-ĸB activation. We performed reporter gene assays and quantitative PCR in A549 and HM3-MUC5AC cells. Results: Both EM and EM900 suppressed IL-1β-induced IL-8 expression in A549 cells. EM900 also suppressed IL-1β-induced IL-1β and TNF-α expression in A549 cells. EM900 inhibited IL-1β-induced MUC5AC expression in HM3-MUC5AC cells. Both EM and EM900 suppressed IL-1β-induced NF-ĸB activation in A549 cells. Conclusion: This study demonstrated that EM900 suppressed the induction of inflammatory cytokines and MUC5AC gene expression in cells derived from human airway epithelia, and our findings indicate that these effects may be mediated by the suppression of NF-ĸB activation.

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Section: Discussionmentioning

confidence: 67%

Effects of a Novel Nonantibiotic Macrolide, EM900, on Cytokine and Mucin Gene Expression in a Human Airway Epithelial Cell Line

et al. 2011

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“…Previous studies have found that ICAM is elevated in patients with CRS compared with controls, 8,24 and levels correlate with tissue levels of neutrophils. 9 Specifically, fibroblasts from nasal polyps were found to induce ICAM expression through production of IL-1␤, leading to increased neutrophil migration.…”

Section: Discussionmentioning

confidence: 83%

Differential Expression of Adhesion Molecules by Sinonasal Fibroblasts among Control and Chronic Rhinosinusitis Patients

Oyer

Nagel

Mulligan

2013

Am J Rhinol�Allergy

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“…Venous blood was collected and centrifuged, and the serum stored at -70 °C until testing. Total serum IgE levels ≥160 IU mL -1 were considered abnormal (13).…”

Section: Allergy Determinationmentioning

confidence: 99%

Eosinophilic Inflammation in Allergic Rhinitis and Nasal Polyposis

Perić

Vojvodić

Vukomanović-Đurđević

et al. 2011

Archives of Industrial Hygiene and Toxicology

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On histopathological examination, nasal polyps and nasal mucosa in allergic rhinitis show different forms of pseudostratifi ed respiratory epithelium, whereas the dominant characteristic of lamina propria is an eosinophilic infi ltration. The aim of this study was to compare interleukin (IL)-5 and eosinophilic cationic protein (ECP) levels in the nasal fl uid of 42 patients: 12 with allergic rhinitis and nasal septal deviation, 17 non-atopic patients with nasal polyposis, and 13 atopic nasal polyp patients were enrolled in this crosssectional study. Nasal secretion samples were collected a few days before surgery. The levels of IL-5 were measured using fl ow cytometry and the ECP using a commercial ELISA kit. In addition, we counted eosinophils in hematoxylin-and-eosin-stained sections of all nasal polyp and all nasal mucosa samples taken from the inferior nasal turbinates during septoplasty. A signifi cantly higher concentration of IL-5 was found in the nasal fl uid of atopic patients with nasal polyposis than in non-atopic nasal polyp patients (p=0.025) and patients with allergic rhinitis (p=0.05). ECP was higher in atopic nasal polyp patients than in patients with allergic rhinitis (p<0.0001) and than in non-atopic nasal polyp patients (p<0.0001). Polyp eosinophils were higher in atopic' than in non-atopic patients (p<0.0001) and higher than in the mucosa of patients with allergic rhinitis (p<0.0001). These however had signifi cantly more mucosal eosinophils than was found in the polyps of non-atopic patients' (p=0.025). ECP levels in nasal fl uid and eosinophil counts in tissue specimens correlated well in all three groups of patients. Our study has shown that atopic nasal polyp patients have a higher level of eosinophilic infl ammation than non-atopic patients with nasal polyps and patients with allergic rhinitis. Arh Hig Rada Tokiskol 2011;62:341-348 Allergic rhinitis is the most common atopic disease in the human population as it affects one in four people worldwide (1, 2). Its prevalence in the European countries ranges between 15 % and 25 %, with a tendency to rise by about 3.5 % with every decade (3). It is a type I allergic reaction mediated by mast cellbound immunoglobulin E (IgE) with a typical helper T-cell type 2 (Th2) profi le (3). KEY WORDS: eosinophilic cationic protein, eosinophils, epithelium, interleukin-5, nasal fl uid Perić A, et al. INFLAMMATORY MEDIATORS IN ALLERGIC RHINITIS AND NASAL POLYPOSISNasal polyposis is characterised by the proliferation of pseudostratifi ed respiratory epithelium, thickening of the basement membrane, oedema, proliferation of fi broblasts, focal fi brosis, and infl ammatory infi ltration of the lamina propria (4). The stromal layer of the polyp tissue includes mixed infl ammatory cells. The predominant histological form of nasal polyposis is the eosinophilic type, with an incidence of about 90 % (5). The non-eosinophilic form of nasal polyps is much less common and the dominant cells in the stroma are lymphocytes and plasmocytes (5). Polyps usually originate ...

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Sinus aspirates in chronic rhinosinusitis: fungal colonization of paranasal sinuses, evaluation of ICAM-1 and IL-8 and studying of immunological effect of long-term macrolide therapy

Abstract: Aspergillus species were the most common. These results confirm the role of ICAM-1 and IL-8 in all types of rhinosinusitis. Erythromycin modulated the immune status of the patients.

Cited by 10 publications

References 0 publications

Effects of a Novel Nonantibiotic Macrolide, EM900, on Cytokine and Mucin Gene Expression in a Human Airway Epithelial Cell Line

Effects of a Novel Nonantibiotic Macrolide, EM900, on Cytokine and Mucin Gene Expression in a Human Airway Epithelial Cell Line

Differential Expression of Adhesion Molecules by Sinonasal Fibroblasts among Control and Chronic Rhinosinusitis Patients

Eosinophilic Inflammation in Allergic Rhinitis and Nasal Polyposis

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