Sinomenine induces apoptosis of prostate cancer cells by blocking activation of NF-kappa B

Abstract

Sinomenine (SN) is an alkaloid that has previously been shown to have a role in such varied processes as inflammation, angiogenesis, arthritis and immunosuppression. Therefore, in this study, possible anti-tumor effects of SN on the human prostate cancer cell lines, PC-3 and DU-145, was investigated. After treatment with varying doses of SN, apoptosis was measured by flow cytometry. Prostaglandin E2 production was detected by ELISA, and expression levels of cyclooxygenase-2 and nuclear factorkappa B were evaluated by western blot. In both PC-3 and DU-145 cells, SN treatment induced apoptosis, inhibited in vitro production of prostaglandin E2, and decreased activation of cyclooxygenase-2 and nuclear factor-kappa B. Growth inhibition assays were also performed to determine whether cell viability was responsible for the decrease in the activation observed. In combination, these results suggest that SN can induce an anti-tumor response by suppressing the activation of cyclooxygenase-2 and nuclear factor-kappa B signaling pathways.

MiR-4646-5p Acts as a Tumor-Suppressive Factor in Triple Negative Breast Cancer and Targets the Cholesterol Transport Protein GRAMD1B

“…GEFs catalyze the activation of the members of the Rho GTPase family, which constitute central players in actin cytoskeleton remodeling and focal cell adhesion [55,56]. Both ARAP3 and ARHGEF2 have been associated with increased migration, invasion, and metastatic behavior in various cancer types [57][58][59][60][61]. The downregulation of these migration-and metastasis-associated factors may thus help to partly explain the reduced migration we observed upon miR-4646-5p mimic transfection.…”