Sinomenine ameliorates arthritis via MMPs, TIMPs, and cytokines in rats

Zhou, Hua; Wong, Yuen Fan; Wang, Jue; Cai, Xiong; Liu, Liang

doi:10.1016/j.bbrc.2008.08.153

Cited by 89 publications

(68 citation statements)

References 27 publications

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“…MMP-2 expression is triggered by macrophages migration inhibitory factor and other cytokines or soluble factors, such as IL-1b and cyclophillin A, which stimulate the expression of the glycoprotein EMMPRIN (CD147) on the cell surface of macrophages, thus inducing MMPs expression (Ahmed et al, 2006;Pakozdi et al, 2006;Zhou et al, 2008;Zhu et al, 2006).…”

Section: Biological Aspectsmentioning

confidence: 99%

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Sbardella

Fasciglione

Gioia

et al. 2012

Molecular Aspects of Medicine

208

212

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a b s t r a c tHuman matrix metalloproteinases (MMPs) belong to the M10 family of the MA clan of endopeptidases. They are ubiquitarian enzymes, structurally characterized by an active site where a Zn 2+ atom, coordinated by three histidines, plays the catalytic role, assisted by a glutamic acid as a general base. Various MMPs display different domain composition, which is very important for macromolecular substrates recognition. Substrate specificity is very different among MMPs, being often associated to their cellular compartmentalization and/or cellular type where they are expressed. An extensive review of the different MMPs structural and functional features is integrated with their pathological role in several types of diseases, spanning from cancer to cardiovascular diseases and to neurodegeneration. It emerges a very complex and crucial role played by these enzymes in many physiological and pathological processes.

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Section: Biological Aspectsmentioning

confidence: 99%

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Sbardella

Fasciglione

Gioia

et al. 2012

Molecular Aspects of Medicine

208

212

View full text Add to dashboard Cite

show abstract

“…The amount of phosphatidylserine exposed on the outside of the apoptotic cells was determined by an Annexin V-FITC Apoptosis Kit. Briefly, following treatment with Sin (120 and 200 µg/ml) for 48 h, 1x10 6 cells were harvested by centrifugation (1,000 rpm/min), washed twice with ice-cold PBS, pelleted and resuspended in 100 µl Annexin V-FITC/PI binding buffer. Following the addition of 5 µl Annexin V-FITC conjugate and 10 µl Pi buffer, the cells were incubated at room temperature for 15 min in the dark.…”

Section: Methodsmentioning

confidence: 99%

“…caspase activity was measured using the Caspase Activity Assay according to the manufacturer's instructions. Briefly, 1x10 6 cells were incubated with designated concentrations of Sin (120 and 200 µg/ml) for 24 h. The cells were washed with ice-cold PBS, resuspended in lysis buffer and left on ice for 15 min. The lysate was centrifuged at 12,000 rpm/min at 4˚C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

“…For pharmaceutical purposes, the hydrochloride chemical form of SIN is generally used. Previous reports have demonstrated that Sin has a wide range of pharmacological actions, including anti-inflammatory (4), antirheumatic (5,6), analgesic (7,8), antiarrhythmic (9), anti-angiogenesis (10), anti-lipidperoxidative (4,11) and immunosuppressive effects (12). in a few studies, Sin has also been reported to have an antitumor effect (13,14).…”

Section: Introductionmentioning

confidence: 99%

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Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro

Jiang

Zhou²,

Zhang³

et al. 2009

Mol Med Rep

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Abstract. Sinomenine (Sin) is an herbal ingredient isolated from Sinomenium acutum Rehd. et Wils. We evaluated the effects of Sin on the growth and apoptosis of human lung cancer nci-H460 cells. Sin inhibited the proliferation of the cells in a dose-and time-dependent manner. apoptosis was confirmed by flow cytometry, the TUNEL assay and transmission electron microscopy. SIN-induced apoptosis was accompanied by the collapse of mitochondrial membrane potential, the release of cytochrome C and the activation of caspase-9 and -3. Sin also increased Bax and decreased Bcl-2 protein levels in the NCI-H460 cells. Data analysis suggested that SIN exerted a significant apoptotic effect on NCI-H460 cells through the mitochondrial pathway. SIN is therefore a promising chemopreventive candidate for lung carcinoma.

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“…Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .…”

Section: Introductionmentioning

confidence: 99%

Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

Chen

et al. 2009

Acta Pharmacol Sin

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Aim:The aim of this study was to investigate the mechanism of the effects of Sinomenine (SIN) on the invasion and migration ability of activated human monocytic THP-1 cells (A-THP-1). Sinomenine is a pure alkaloid extracted from the Chinese medical plant Sinomenium acutum. Methods: Human monocytic THP-1 cells were induced to differentiate into macrophages with phorbol 12-myristate 13-acetate (PMA). Cells were treated with different concentrations of SIN. The invasion and migration ability of cells was tested by in vitro transwell assays. The levels of CD147 and MMPs were evaluated by flow cytometric analysis and zymographic analysis, respectively. The mRNA expression of CD147, MMP-2, and MMP-9 was measured by RT-PCR. Results:The invasion and migration ability of A-THP-1 cells was significantly inhibited by SIN in a concentration-dependent fashion; at the same time, the levels of CD147, MMP-2, and MMP-9 were markedly down-regulated. This inhibitory effect was most notable at concentrations of 0.25 mmol/L and 1.00 mmol/L (P<0.01). Conclusion: A possible mechanism of the inhibitory effect of SIN on cell invasion and migration ability is repression of the expression of MMP-2 and MMP-9, which strongly correlates with the inhibition of CD147 activity. IntroductionSinomenine (SIN,7, C 19 H 23 NO 4 ), an alkaloid isolated from the Chinese medicinal plant, Sinomenium acutum, has been utilized to safely treat arthritis for many years. Previous studies have demonstrated that SIN has a variety of pharmacological effects, including anti-inflammatory effects, immunosuppression, and prevention of cartilage destruction [1][2][3][4] . As an immunosuppressive agent, SIN has been used extensively in China for the treatment of rheumatoid arthritis [5,6] .Rheumatoid arthritis (RA) is a systemic, immune and inflammatory disease characterized by joint swelling, synovial inflammation and joint destruction, leading to significant disability [7] . The regulation of cell migration and invasion is a critical process throughout the development of RA.Enhanced migration and invasion of peripheral macrophages contribute to joint destruction in RA by directly degrading the cartilage matrix and indirectly promoting angiogenesis. Elevated gene expression of gelatinase A (also called matrix metalloproteinase-2, MMP-2) and gelatinase B (also called matrix metalloproteinase-9, MMP-9) is critical for the progression of RA [8] .The extracellular matrix metalloproteinase inducer (EMMPRIN, CD147) is a heavily glycosylated protein containing two immunoglobulin super family domains. It is enriched on the surface of macrophages and is associated with differentiation of human monocytic THP-1 cells when treated with phorbol 12-myristate 13-acetate (PMA). This protein stimulates the production of matrix metalloproteinases (MMPs). CD147 has also been reported to play an important role in the invasion and migration ability of cells in both animal models and cancer patients [9] .Although many in vivo studies have demonstrated that SIN can significantly improve...

show abstract

Sinomenine ameliorates arthritis via MMPs, TIMPs, and cytokines in rats

Cited by 89 publications

References 27 publications

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Human matrix metalloproteinases: An ubiquitarian class of enzymes involved in several pathological processes

Effects of sinomenine on proliferation and apoptosis in human lung cancer cell line NCI-H460 in vitro

Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147

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