Sink filling, inulin metabolizing enzymes and carbohydrate status in field grown chicory (Cichorium intybus L.)

Development of rubber‐enriched dandelion varieties by metabolic engineering of the inulin pathway

“…Dandelion inulin crystals are clustered in parenchymal root cell vacuoles close to phloem tissues adjacent to laticifers (Javorsky, 1944; Van den Ende et al ., 2000b) (Figure 1). The abundance of photoassimilates during the summer leads to the continuous accumulation of inulin during vegetative growth, as shown in chicory, Jerusalem artichoke and dandelions (van Arkel et al ., 2012; Javorsky, 1944; Marx et al ., 1997; Schorr‐Galindo and Guiraud, 1997; Van den Ende et al ., 2000b). In autumn, declining levels of photoassimilates promote inulin degradation, and root tissue thus undergoes a sink‐to‐source transition (De Roover et al ., 1999; Van den Ende and Van Laere, 1996; Van den Ende et al ., 2000b).…”

A one-step bioprocess for production of high-content fructo-oligosaccharides from inulin by yeast

“…Inulin is a naturally occurring storage polysaccharide present in numerous plants, such as Jerusalem artichoke and chicory (Li, Zhang et al, 2015;van Arkel et al, 2012). Inulin consists of linear chains of β-2,1-linked D-fructofuranose molecules terminated by a glucose residue.…”

The hot, the cold and the tulip : the regulation of flowering time and dormancy release

“…Total carbohydrate content was extracted from freeze-dried bulb scale, stem, leaf and floral bud tissue according to van Arkel et al (2012). 10mg dry weight of each tissue was mixed with 600 µl 20 mM phosphate buffer (pH 7) and incubated for 30 min at 85°C in thermomixer (Vortemp, Labnet) with shaking every 5 min .…”

“…The content of glucose, fructose and sucrose was determined with the Dionex ICS-5000+ HPIC equipped with a CarbopacTM PA1 (Thermo Scientific, The Netherlands). The gradient and column specifications and quantification methods are described in (van Arkel et al, 2012).…”