Sinigrin and sinalbin quantification in mustard seed using high performance liquid chromatography–time-of-flight mass spectrometry

Popova, Inna E.; Morra, Matthew J.

doi:10.1016/j.jfca.2014.04.011

Cited by 24 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 1 H, 13 C and 2D: heteronuclea single quantum coherence (HSQC) and correlation spectroscopy (COSY) NMR analyses provided clear peaks that differentiated the rhamnose to glucose moieties in addition to other peaks typical of aromatic rings, particularly benzene, which signifies the presence of glucosinolates containing sugar moieties. HPLC analysis of GMG-ITC-RSE revealed a peak around 4.940 min (retention time) at 245 nm, similar to that of sinigrin (4.940 min), the internal standard ( Figure 2 ), as reported by [ 47 ], which indicated the presence of glucosinolates in the extract. Identification of the M. oleifera derived glucosinolate under investigation was further corroborated by mass spectrometric examination where LC/MS ESI analyses in the positive mode revealed an m / z 588 (M + H + ) ( Figure 3 ), which corresponded to the mass of intact GMG ( Figure 1 ) reported by [ 48 ].…”

Section: Resultssupporting

confidence: 78%

Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

et al. 2018

View full text Add to dashboard Cite

The incidence of prostate cancer malignancy along with other cancer types is increasing worldwide, resulting in high mortality rate due to lack of effective medications. Moringa oleifera has been used for the treatment of communicable and non-communicable ailments across tropical countries, yet, little has been documented regarding its effect on prostate cancer. We evaluated the acute toxicity and apoptosis inducing effect of glucomoringin-isothiocyanate rich soluble extracts (GMG-ITC-RSE) from M. oleifera in vivo and in vitro, respectively. Glucomoringin was isolated, identified, and characterized using fundamental analytical chemistry tools where Sprague-Dawley (SD) rats, murine fibroblast (3T3), and human prostate adenocarcinoma cells (PC-3) were used for acute toxicity and bioassays experiments. GMG-ITC-RSE did not instigate adverse toxic reactions to the animals even at high doses (2000 mg/kg body weight) and affected none of the vital organs in the rats. The extract exhibited high levels of safety in 3T3 cells, where more than 90% of the cells appeared viable when treated with the extract in a time-dependent manner even at high dose (250 µg/mL). GMG-ITC-RSE significantly triggered morphological aberrations distinctive to apoptosis observed under microscope. These findings obviously revealed the putative safety of GMG-ITC-RSE in vivo and in vitro, in addition to its anti-proliferative effect on PC-3 cells.

show abstract

Section: Resultssupporting

confidence: 78%

Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The MSP was made by directly grinding mustard seeds into flour, thus the Myr activity was preserved, but the major GSLs in mustard seed are sinigrin and sinalbin, not GR. 27 As anticipated, GR and SF were not detected in the MSP. The extraction recovery for GR and SF was 95.6% and 98.8%, respectively.…”

Section: Intraday Accuracy/precision Bsupporting

confidence: 57%

“…A very trace amount of SF was observed in the BSE powder (0.12 mg per 1000 mg of fresh powder weight). The MSP was made by directly grinding mustard seeds into flour, thus the Myr activity was preserved, but the major GSLs in mustard seed are sinigrin and sinalbin, not GR . As anticipated, GR and SF were not detected in the MSP.…”

Section: Resultsmentioning

confidence: 82%

“…Moreover, methods will facilitate studies focusing on the design of delivery methods that improve conversion of GR to SF in the gut. For example, the main GSLs in mustard seed is sinigrin and sinalbin, and there are negligible levels of GR in mustard seed, but mustard seed is a rich source of Myr and is frequently processed into mustard seed powder (MSP) and given with broccoli sprouts or broccoli seed extract (BSE) to enhance the conversion of GR to SF. − …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Robust UHPLC-(ESI+)-MS/MS Method for Simultaneous Analysis of Glucoraphanin, Sulforaphane, and Sulforaphane Metabolites in Biological Samples

Zhu

Lerno

Cremonini

et al. 2023

ACS Food Sci. Technol.

View full text Add to dashboard Cite

A UHPLC-(ESI+)-MS/MS method was developed and validated for the simultaneous determination of glucoraphanin (GR), sulforaphane (SF), sulforaphane-glutathione (SF-GSH), sulforaphane-cysteine (SF-CYS), and sulforaphane-N-acetyl-cysteine (SF-NAC), in a range of biological materials (digesta, cell culture medium, cell lysates, urine) with no sample pretreatment. The limit of detection (LODs) ranged from 0.03 to 1.95 μg L–1. The intra- and interday variation was ≤15% and recoveries were ≥85%. Linearity ranged from 3.90 to 1000 μg L–1 (GR), 0.06–500 μg L–1 (SF), 3.90–1000 μg L–1 (SF-NAC), and 62.5–500 μg L–1 for SF-CYS and SF-GSH. The method was validated in broccoli seed powders, and by determining transport and metabolism of SF in Caco-2 monolayers, and model digesta. Results indicated that 72.4% of SF was absorbed, transported, and metabolized (SF-CYS, SF-GSH, SF-NAC) in the Caco-2 monolayers after 4 h of incubation. This is a robust and time- and cost-efficient method that is suitable for high throughput applications.

show abstract

“…Linseed meal flake sizes ranged from %0.07 to 1.5 mm diameter. Meals were analyzed for sinigrin and sinalbin content following the methods in Popova and Morra (2014). For the greenhouse studies, glucosinolate content was 132 mmolÁg -1 meal for YeM and 149 mmolÁg -1 meal for InM (total meal basis).…”

Section: Southernmentioning

confidence: 99%

Mustard Seed Meal for Management of Root-knot Nematode and Weeds in Tomato Production

Meyer

Zasada

Rupprecht

et al. 2015

hortte

Self Cite

View full text Add to dashboard Cite

Mustard seed meals of indian mustard [InM (Brassica juncea)] and yellow mustard [YeM (Sinapis alba)], alone and combined, were tested for effects on tomato (Solanum lycopersicum) plants and for suppression of southern root-knot nematode [RKN (Meloidogyne incognita)] and weed populations. In the greenhouse, with all seed meal treatments applied at 0.25% total w/w soil, low tomato plant stands (up to 60% dying/dead) resulted from amendment with 3 YeM:1 InM, 1 YeM:1 InM, and YeM, applied right before transplant. Compared with untreated controls, low numbers of RKN eggs per gram root were consistently recorded from amendment with 3 YeM:1 InM. In a 2012 field study, incorporation of 1 YeM:1 InM (1700 lb/acre) resulted in lower tomato root biomass than fertilizer application (504 lb/acre), YeM or InM (each 1700 lb/acre). All treatments were applied with added fertilizer to achieve 100–102 lb/acre nitrogen, 7.4 lb/acre phosphorus, 74.7 lb/acre potassium, 6.0 lb/acre sulfur, and 1.0 lb/acre boron. The lowest numbers of RKN eggs per gram root (harvest 2012) were collected from plots amended with InM (1700 lb/acre), YeM (850 lb/acre), and 3 YeM:1 InM (1700 lb/acre), but the numbers were not significantly different from fertilizer only (504 lb/acre) controls. Highest and lowest tomato yields (numbers of fruit) in 2012 were recorded from YeM (850 lb/acre) and 3 YeM:1 InM (1700 lb/acre) amendments, respectively. In 2013, there were no significant differences among treatments in eggs per gram root or in tomato yields. No mustard seed meal treatment affected weed populations. At the tested rates, YeM seed meal showed potential for use in tomato beds but results were inconsistent between years.

show abstract

Sinigrin and sinalbin quantification in mustard seed using high performance liquid chromatography–time-of-flight mass spectrometry

Cited by 24 publications

References 26 publications

Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

Nontoxic Glucomoringin-Isothiocyanate (GMG-ITC) Rich Soluble Extract Induces Apoptosis and Inhibits Proliferation of Human Prostate Adenocarcinoma Cells (PC-3)

Robust UHPLC-(ESI+)-MS/MS Method for Simultaneous Analysis of Glucoraphanin, Sulforaphane, and Sulforaphane Metabolites in Biological Samples

Mustard Seed Meal for Management of Root-knot Nematode and Weeds in Tomato Production

Contact Info

Product

Resources

About