Singlet oxygen-induced inhibition of cardiac sarcolemmal Na+K+-ATPase

Vinnikova, Anna K.; Kukreja, Rakesh C.; Heß, Michael

doi:10.1016/0022-2828(92)91835-s

Cited by 33 publications

(11 citation statements)

References 27 publications

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“…It has been previously reported by Vinnikova et al (1992) and Hitschke et al (1994) that Na / /K / -ATPase pump activity was susceptible to radiation and chemically-induced ROS. The data presented here confirm that ROS significantly affects Na / /K / -ATPase pump activity in irradiated PW cells, and are consistent with previously reported observations.…”

Section: Rbmentioning

confidence: 81%

“…Radiation or chemically induced ROS have been redemonstrate the increased level of Na / /K / -ATPase activity in the Bcl-2 overexpressing cells compared to the ported to inhibit Na / /K / -ATPase pump activity (Hitschke et al, 1994;Vinnikova et al, 1992). In order parental control cells.…”

Section: Rbmentioning

confidence: 98%

“…Indeed, an association between the Na / /K / -ATPase pump and radiation damage was initially sug-St. Louis, MO) and 11 for 10 min in TBS. The filter was then incubated with horseradish peroxidase congested by the observations of Vinnikova et al (1992) and Hitschke et al (1994) …”

mentioning

confidence: 99%

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Influence of Bcl-2 overexpression on Na+/K+-ATPase pump activity: Correlation with radiation-induced programmed cell death

Gilbert

Knox

1997

J. Cell. Physiol.

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Bcl-2 overexpression in transfected PW cells is associated with inhibition of radiation-induced programmed cell death (PCD). We have previously reported that there is a relationship between inhibition of radiation-induced PCD and membrane hyperpolarization in these cells. In this article, we report that Na+/ K(+)-ATPase pump activity, as measured by the uptake of Rubidium-86 (86Rb+), is significantly higher in Bcl-2 overexpressing PW cells than in control PW cells, and that pump activity following irradiation with doses > or = 500 cGy was reduced to a lesser extent in the Bcl-2 transfectants than in the control cells. When PW-Bcl-2 cells were incubated with a dose of ouabain (1 microM) that decreased pump activity significantly, but did not induce PCD, the previously reported protection from radiation-induced PCD associated with overexpression of Bcl-2 no longer existed. In order to demonstrate that reactive oxygen species (ROS) affected Na+/ K(+)-ATPase pump activity, cells were incubated with N-acetyl cysteine (NAC) prior to irradiation, or treated with the ROS generating drug buthionine sulphoxamine (BSO). 86Rb+ uptake was significantly higher in irradiated cells incubated with NAC compared to cells irradiated in the absence of NAC, while BSO resulted in lower levels of 86Rb+ uptake, suggesting that the effects of radiation on the Na+/K(+)-ATPase pump were due to ROS. Furthermore, the resting cell membrane potential of cells exposed to NAC were slightly hyperpolarized compared to control PW cells, whereas cells exposed to BSO were depolarized in comparison to control PW cells. In summary, this data suggests that Bcl-2 affects Na+/K(+)-ATPase pump activity, which is associated with the resting membrane potential and the level of susceptibility to radiation-induced PCD.

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Section: Rbmentioning

confidence: 81%

Section: Rbmentioning

confidence: 98%

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Influence of Bcl-2 overexpression on Na+/K+-ATPase pump activity: Correlation with radiation-induced programmed cell death

Gilbert

Knox

1997

J. Cell. Physiol.

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“…It is conceivable that Bcl-2 may interact with the Na'/K'-ATPase pump, resulting in conformational changes that may affect the level of activity of the ATPase and the ability of ouabain to bind to the extracellular domain. A link between the Na+/K+-ATPase pump and radiosensitivity was suggested by the observations of Vinnikova et al (1992) and Hitschke et al (19941, who demonstrated that the Na +/K+ -ATPase pump is susceptible to inactivation by radiation-induced production of reactive oxygen species (ROS). The data presented in this paper suggests that there is an association between Bcl-2 overexpression, membrane hyperpolarization, and increased radioresistance and that Bcl-2 overexpression may affect the Alternatively, since Bcl-2 has been shown to interact with other intracellular proteins, such protein-protein interactions may themselves be important in the maintenance of the membrane potential.…”

Section: Discussionmentioning

confidence: 95%

Association of BCL-2 with membrane hyperpolarization and radioresistance

et al. 1996

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The resting membrane potential of parental, neomycin control, and Bcl-2 transfected cells was measured, and the effect of membrane hyperpolarization or depolarization on radiosensitivity was studied. Bcl-2 transfected cells were significantly more radioresistant than control cells and were significantly hyperpolarized compared to parental and neomycin control transfected PW and HL60 cells. Hyperpolarization of the parental and neomycin control transfected cells by valinomycin significantly increased the radioresistance of these cells to such an extent that there was no longer a significant difference in the survival of the valinomycin treated and irradiated control cells compared to similarly irradiated Bcl-2 transfected cells. In contrast, depolarization of the Bcl-2 transfected PW and HL60 cells decreased the radioresistance of the Bcl-2 transfectants to a level similar to that of the control cells. The data presented here suggest that overexpression of Bcl-2 affects membrane potential and that this hyperpolarization is associated with increased radioresistance of cells that overexpress Bcl-2. Furthermore, Bcl-2 transfected cells were also less susceptible to the specific Na+/K(+)-ATPase inhibitor ouabain, suggesting that Bcl-2 may act at the level of the Na+/K(+)-ATPase pump.

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“…It possesses vasodilating and hypotensive actions and may boost the activity of soothing alpha waves in the brain. His is used in the treatment of anemia, allergies, rheumatoid arthritis, and other inflammatory reactions [24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Speciation Studies of L-Histidine Complexes of Pb(II), Cd(II), and Hg(II) in DMSO-Water Mixtures

Naik

Kumar

Raju

et al. 2012

International Journal of Inorganic Chemistry

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, and ML. The appropriateness of experimental conditions is verified by introducing errors intentionally in the concentrations of ingredients. The models containing different numbers of species were refined by using the computer program MINIQUAD75. The best-fit chemical models were arrived at based on statistical parameters. The trend in variation of stability constants of the complexes with dielectric constant of the medium is attributed to the electrostatic and nonelectrostatic forces. The species distribution and the plausible equilibria for the formation of the species are also presented.

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Singlet oxygen-induced inhibition of cardiac sarcolemmal Na+K+-ATPase

Cited by 33 publications

References 27 publications

Influence of Bcl-2 overexpression on Na+/K+-ATPase pump activity: Correlation with radiation-induced programmed cell death

Influence of Bcl-2 overexpression on Na+/K+-ATPase pump activity: Correlation with radiation-induced programmed cell death

Association of BCL-2 with membrane hyperpolarization and radioresistance

Speciation Studies of L-Histidine Complexes of Pb(II), Cd(II), and Hg(II) in DMSO-Water Mixtures

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