Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells

Sanpui, Pallab; Zheng, Xiao; Loeb, Julia C.; Bisesi, Joseph H.; Khan, Iftheker A.; Afrooz, A. R. M. Nabiul; Liu, Keira; Badireddy, Appala Raju; Wiesner, Mark R.; Ferguson, P. Lee; Saleh, Navid B.; Lednicky, John A.; Sabo‐Attwood, Tara

doi:10.1186/s12989-014-0066-0

Cited by 40 publications

(34 citation statements)

References 63 publications

(68 reference statements)

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“…For example, it has been demonstrated that exposure of cells to single-walled carbon nanotubes increases the susceptibility of these cells to infection with influenza H1N1 [49]. Additionally, it has been shown that preexposure of mice to carbon black prior to infection with respiratory syncytial virus (RSV) induces an inflammatory milieu that promotes disease exacerbation [50].…”

Section: Discussionmentioning

confidence: 99%

Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection

et al. 2017

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BackgroundInhalation of environmental (nano) particles (NP) as well as persistent herpesvirus-infection are potentially associated with chronic lung disease and as both are omnipresent in human society a coincidence of these two factors is highly likely. We hypothesized that NP-exposure of persistently herpesvirus-infected cells as a second hit might disrupt immune control of viral latency, provoke reactivation of latent virus and eventually lead to an inflammatory response and tissue damage.ResultsTo test this hypothesis, we applied different NP to cells or mice latently infected with murine gammaherpesvirus 68 (MHV-68) which provides a small animal model for the study of gammaherpesvirus-pathogenesis in vitro and in vivo. In vitro, NP-exposure induced expression of the typically lytic viral gene ORF50 and production of lytic virus. In vivo, lytic viral proteins in the lung increased after intratracheal instillation with NP and elevated expression of the viral gene ORF50 could be detected in cells from bronchoalveolar lavage. Gene expression and metabolome analysis of whole lung tissue revealed patterns with striking similarities to acute infection. Likewise, NP-exposure of human cells latently infected with Epstein-Barr-Virus also induced virus production.ConclusionsOur results indicate that NP-exposure of persistently herpesvirus-infected cells – murine or human – restores molecular signatures found in acute virus infection, boosts production of lytic viral proteins, and induces an inflammatory response in the lung – a combination which might finally result in tissue damage and pathological alterations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12989-016-0181-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection

et al. 2017

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“…Freese et al ( 2014 ) investigate SiO 2 NP uptake into human umbilical vein endothelial cells (HUVEC) cultivated under cyclic stretch conditions. Stretch (5%) was applied to HUVEC grown on a flexible silicon membrane subject to cyclic elongation with a 1Hz frequency and exposed to a growth medium for 48 h. Different sizes and surface modifications of SiO 2 NP surface (−COOH, −NH 2 , and –OH groups) were investigated.…”

Section: Introductionmentioning

confidence: 99%

Influence of shear stress and size on viability of endothelial cells exposed to gold nanoparticles

et al. 2017

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Screening nanoparticle toxicity directly on cell culture can be a fast and cheap technique. Nevertheless, to obtain results in accordance with those observed in live animals, the conditions in which cells are cultivated should resemble the one encountered in live systems. Microfluidic devices offer the possibility to satisfy this requirement, in particular with endothelial cell lines, because they are capable to reproduce the flowing media and shear stress experienced by these cell lines in vivo. In this work, we exploit a microfluidic device to observe how human umbilical vein endothelial cells (HUVEC) viability changes when subject to a continuous flow of culture medium, in which spherical citrate-stabilized gold nanoparticles of different sizes and at varying doses are investigated. For comparison, the same experiments are also run in multiwells where the cells do not experience the shear stress induced by the flowing medium. We discuss the results considering the influence of mode of exposure and nanoparticle size (24 and 13 nm). We observed that gold nanoparticles show a lower toxicity under flow conditions with respect to static and the HUVEC viability decreases as the nanoparticle surface area per unit volume increases, regardless of size.Electronic supplementary materialThe online version of this article (10.1007/s11051-017-3993-5) contains supplementary material, which is available to authorized users.

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“…For example, it has been demonstrated that small airway epithelial cells infected with IAV produce increased titers only when exposed to pristine SWCNTs prior to infection. The noted increase in IAV titers could not be duplicated with a simultaneous dual exposure or by exposing the cells to the SWCNTs after infection (Sanpui et al 2014). However, in another study, DWCNTs were able to reactivate latent herpesvirus, suggesting that the infections occurred prior to NP exposure (Sattler et al 2017).…”

Section: Nanoparticle and Viral Exposure Routes And Scenariosmentioning

confidence: 96%

“…Another interesting aspect of these studies showed that the effects of carbon NMs on viral infection varied by the type of nanoparticles tested. Exposure to both single-walled carbon nanotubes (SWCNTs) was able to increase virus titers of IAV (Sanpui et al 2014;Chen et al 2017). Interestingly, in another study, exposure of mice to multiwalled carbon nanotubes (MWCNTs) exacerbated pneumonia and immune cell infiltration in lungs of RSVinfected mice but did not influence the virus titers compared with RSV treatment only (Hashiguchi et al 2015a).…”

Section: Nanoparticle Exposure and Viral Infectionmentioning

confidence: 98%

“…There is not an abundance of evidence to suggest that classic additive, synergistic, or antagonistic toxicity occurs under any of these exposure scenarios, but this observation likely depends on the endpoints that are quantified, which have been limited. However, mixed injury profiles may be much more detrimental compared with effects caused by exposure to single agents (Sanpui et al 2014;Chen et al 2017;Walling and Lau 2014;Shvedova et al 2008). Noteworthy, the exposure scenarios described all likely occur in occupational and environmental settings on some level.…”

Section: Nanoparticle and Viral Exposure Routes And Scenariosmentioning

confidence: 99%

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Nanomaterial Effects on Viral Infection

Chen

Humes

Saleh

et al. 2020

Interaction of Nanomaterials With the Immune System

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The potential for environmental and occupational exposures of populations to nanomaterials (NMs) has fostered concerns of associated adverse health effects, with a particular emphasis on pulmonary injury and disease. Many studies have revealed that several types of NMs can evoke a variety of biological responses, such as pulmonary inflammation and oxidative stress, which contribute to allergy, fibrosis, and granuloma formation. Less attention has been paid to health effects that may result from exposure to NMs and additional stressors such as pathogens, with a particular focus on susceptibility to viral infection. This chapter will summarize the current body of literature related to NMs and viral exposures with a primary focus on immune modulation. A summary of the studies performed and major findings to date will be discussed, highlighting proposed molecular mechanisms behind NM-driven host susceptibility, challenges, limitations, and future research needs. Specific mechanisms discussed include direct interaction between NMs and biological molecules, activation of pattern recognition receptors (PRRs) and related signaling pathways, production of oxidative stress and mitochondrial dysfunction, inflammasome activation, and modulation of lipid signaling networks.

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Single-walled carbon nanotubes increase pandemic influenza A H1N1 virus infectivity of lung epithelial cells

Cited by 40 publications

References 63 publications

Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection

Nanoparticle exposure reactivates latent herpesvirus and restores a signature of acute infection

Influence of shear stress and size on viability of endothelial cells exposed to gold nanoparticles

Nanomaterial Effects on Viral Infection

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