Single-vesicle measurement of protein-induced membrane tethering

Cai, Bin; Yu, Luning; Sharum, Savanna R.; Zhang, Kai; Diao, Jiajie

doi:10.1016/j.colsurfb.2019.02.004

Cited by 4 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vesicles with different compositions were synthesized according to established protocols. 36 Glass vials and glass syringes were washed three times with Milli-Q water, ethanol (100%, Fisher Scientific, USA), and chloroform (Fisher Scientific, USA) before use. Lipids (Avanti Polar Lipids, Inc, USA) were Table 1.…”

Section: ■ Introductionmentioning

confidence: 99%

Coaction of Electrostatic and Hydrophobic Interactions: Dynamic Constraints on Disordered TrkA Juxtamembrane Domain

Wang

Fan

et al. 2019

J. Phys. Chem. B

Self Cite

View full text Add to dashboard Cite

The receptor tyrosine kinase family transmits signals into cell via a single transmembrane helix and a flexible juxtamembrane domain (JMD). Membrane dynamics makes it challenging to study the structural mechanism of receptor activation experimentally. In this study, we employ all-atom molecular dynamics with Highly Mobile Membrane-Mimetic to capture membrane interactions with the JMD of tropomyosin receptor kinase A (TrkA). We find that PIP2 lipids engage in lasting binding to multiple basic residues and compete with salt bridge within the peptide. We discover three residues insertion into the membrane, and perturb it through computationally designed point mutations. Single-molecule experiments indicate the contribution from hydrophobic insertion is comparable to electrostatic binding, and in-cell experiments show that enhanced TrkA-JMD insertion promotes receptor ubiquitination. Our joint work points to a scenario where basic and hydrophobic residues on disordered domains interact with lipid headgroups and tails, respectively, to restrain flexibility and potentially modulate protein function.

show abstract

Section: ■ Introductionmentioning

confidence: 99%

Coaction of Electrostatic and Hydrophobic Interactions: Dynamic Constraints on Disordered TrkA Juxtamembrane Domain

Wang

Fan

et al. 2019

J. Phys. Chem. B

Self Cite

View full text Add to dashboard Cite

show abstract

“…The growing process could also be light-controlled through the use of photo-sensitive surfactants ( Suzuki et al, 2017 ). Besides, the protein-lipid interaction was proved promising for the induction of membrane tethering and fusion ( Cai et al, 2019 ). Some polymer-based vesicles could grow upon integrating stimuli-responsive nanoparticles, which contained the membrane materials ( Schwille et al, 2018 ).…”

Section: Modularizationmentioning

confidence: 99%

Modularize and Unite: Toward Creating a Functional Artificial Cell

Wang

Yang

2021

Front. Mol. Biosci.

View full text Add to dashboard Cite

An artificial cell is a simplified model of a living system, bringing breakthroughs into both basic life science and applied research. The bottom-up strategy instructs the construction of an artificial cell from nonliving materials, which could be complicated and interdisciplinary considering the inherent complexity of living cells. Although significant progress has been achieved in the past 2 decades, the area is still facing some problems, such as poor compatibility with complex bio-systems, instability, and low standardization of the construction method. In this review, we propose creating artificial cells through the integration of different functional modules. Furthermore, we divide the function requirements of an artificial cell into four essential parts (metabolism, energy supplement, proliferation, and communication) and discuss the present researches. Then we propose that the compartment and the reestablishment of the communication system would be essential for the reasonable integration of functional modules. Although enormous challenges remain, the modular construction would facilitate the simplification and standardization of an artificial cell toward a natural living system. This function-based strategy would also broaden the application of artificial cells and represent the steps of imitating and surpassing nature.

show abstract

Single-vesicle imaging quantifies calcium’s regulation of nanoscale vesicle clustering mediated by α-synuclein

et al. 2020

View full text Add to dashboard Cite

Although numerous studies have shown that the protein α-synuclein (α-Syn) plays a central role in Parkinson’s disease, dementia with Lewy bodies, and other neurodegenerative diseases, the protein’s physiological function remains poorly understood. Furthermore, despite recent reports suggesting that, under the influence of Ca2+, α-Syn can interact with synaptic vesicles, the mechanisms underlying that interaction are far from clear. Thus, we used single-vesicle imaging to quantify the extent to which Ca2+ regulates nanoscale vesicle clustering mediated by α-Syn. Our results revealed not only that vesicle clustering required α-Syn to bind to anionic lipid vesicles, but also that different concentrations of Ca2+ exerted different effects on how α-Syn induced vesicle clustering. In particular, low concentrations of Ca2+ inhibited vesicle clustering by blocking the electrostatic interaction between the lipid membrane and the N terminus of α-Syn, whereas high concentrations promoted vesicle clustering, possibly due to the electrostatic interaction between Ca2+ and the negatively charged lipids that is independent of α-Syn. Taken together, our results provide critical insights into α-Syn’s physiological function, and how Ca2+ regulates vesicle clustering mediated by α-Syn.

show abstract

Single-vesicle measurement of protein-induced membrane tethering

Cited by 4 publications

References 33 publications

Coaction of Electrostatic and Hydrophobic Interactions: Dynamic Constraints on Disordered TrkA Juxtamembrane Domain

Coaction of Electrostatic and Hydrophobic Interactions: Dynamic Constraints on Disordered TrkA Juxtamembrane Domain

Modularize and Unite: Toward Creating a Functional Artificial Cell

Single-vesicle imaging quantifies calcium’s regulation of nanoscale vesicle clustering mediated by α-synuclein

Contact Info

Product

Resources

About