Single-use porous thin film extraction with gas chromatography atmospheric pressure chemical ionization tandem mass spectrometry for high-throughput analysis of 16 PAHs

Shahhoseini, Fereshteh; Azizi, Ali; Egli, Stefana N.; Bottaro, Christina S.

doi:10.1016/j.talanta.2019.120320

Cited by 30 publications

(6 citation statements)

References 29 publications

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“…Of the microextraction methods, porous thin‐film microextraction (TFME) has been employed most often for environmental samples, 22 , 23 with additional sensitivity and specificity imparted through incorporation of molecular imprinted polymers (MIPs). 24 , 25 , 26 Recently, this approach has been extended to biological samples.…”

Section: Introductionmentioning

confidence: 99%

“…20 One previous study reported a carbowax/templates resin SPME method with HPLC-UV detection for the measurement of MPA in human serum with estimated LOD in plasma of 50 ng mL −1 . 21 Of the microextraction methods, porous thin-film microextraction (TFME) has been employed most often for environmental samples, 22,23 with additional sensitivity and specificity imparted through incorporation of molecular imprinted polymers (MIPs). [24][25][26] Recently, this approach has been extended to biological samples.…”

Section: Introductionmentioning

confidence: 99%

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Development and application of a thin‐film molecularly imprinted polymer for the measurement of mycophenolic acid in human plasma

Langille

Bottaro

2023

Clinical Laboratory Analysis

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Background Mycophenolic acid (MPA) is used to suppress the immune response following organ transplantation; however, complex pharmacokinetic behavior and a large interpersonal variability necessitate therapeutic drug monitoring. To overcome the limitations of current sample preparation techniques, we present a novel thin‐film molecularly imprinted polymer (TF‐MIP) extraction device as part of a simple, sensitive, and fast method for analysis of MPA from human plasma. Methods Mycophenolic acid is extracted from plasma using a tailor‐made TF‐MIP that is subsequently desorbed into an organic solvent system compatible with mass spectrometry. The MIP yielded higher recovery of MPA relative to a corresponding non‐imprinted polymer. The method allows for the determination of MPA in 45 min including analysis time and can be scaled for high throughput to process as many as 96 samples per hour. Results The method gave an LOD of 0.3 ng mL −1 and was linear from 5 to 250 ng mL −1 . Patient plasma samples (35 μL) were diluted using charcoal‐stripped pooled plasma to a final extraction volume of 700 μL; when MPA in patient plasma is high, this ratio can easily be adjusted to ensure samples are within the method linear range. Intra‐ and inter‐day variability were 13.8% and 4.3% (at 15 ng mL −1 ) and 13.5% and 11.0% (at 85 ng mL −1 ), respectively ( n = 3); inter‐device variability was 9.6% ( n = 10). Conclusions Low inter‐device variability makes these devices suitable for single use in a clinical setting, and the fast and robust method is suitable for therapeutic drug monitoring, where throughput and time‐to‐result are critical.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and application of a thin‐film molecularly imprinted polymer for the measurement of mycophenolic acid in human plasma

Langille

Bottaro

2023

Clinical Laboratory Analysis

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“…Compared to GC‐MS, GC‐MS/MS in multiple reaction monitoring (MRM) mode allows for greater compound specificity by eliminating the matrix ions arising from other compounds that share the same parent mass but lack the correct product ion, thereby, drastically reducing the detection limits and decreasing background interference. GC‐MS/MS presents great advantages for analyzing tobacco alkaloids [14], VNAs [6], and PAHs [17, 19–21] in tobacco products and other complex sample matrices.…”

Section: Introductionmentioning

confidence: 99%

Smokeless tobacco analysis: Simultaneous extraction and purification of alkaloids, volatile N‐nitrosamines, and polycyclic hydrocarbons for GC‐MS/MS

Wang

Qin

Nie

et al. 2021

J of Separation Science

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Several smokeless tobacco products are available in the market and comprise complex chemical matrices. Sample preparation for analysis of the multiple classes of harmful compounds in smokeless tobacco products is highly cumbersome. In this study, a simultaneous extraction scheme was developed for three toxic analyte classes in smokeless tobacco products using a two‐phase solution consisting of 5% aqueous NaOH and dichloromethane in a 1:4 ratio. The dichloromethane extract was used to analyze four alkaloids directly at levels greater than parts per million; however, passing the layer through a silica cartridge for further purification and concentration was necessary for determining 18 polycyclic aromatic hydrocarbons and four volatile N‐nitrosoamines at the ppt level. The multitargets were determined by using gas chromatography with tandem mass spectrometry. The limits of detection for the 18 polycyclic aromatic hydrocarbons, four volatile N‐nitrosoamines, three minor alkaloids, and nicotine were 0.2‐1.2, 0.2‐0.4, 0.6‐1.0, and 10.2 μg/g, respectively. Four different smokeless tobacco substrates were fortified with three levels of mixed standards, and the recoveries ranged between 83 and 110%. The method was highly efficient, reduced the sample amounts, solvents, and the time required by approximately 60%. The method was used to assay 18 smokeless tobacco products, and showed potentials in assaying drugs and other plant‐based substrates.

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“…Consequently, the United States Environmental Protection Agency and the European Food Safety Authority have established maximum residue limits for PAHs in foods and reviewed a series of data on their toxicity [11,12]. Different analytical methods such as GC equipped with flame ionization detector [13], MS [14,15], or MS/MS [16] and HPLC equipped with variable wavelength detector [17], diode array detector [18,19], and fluorescence detector [20] have been used for identification and monitoring of PAHs in different samples. Although analytical instruments have a key role in monitoring PAHs, performing sample preparation methods is inevitable to remove interferences and the analytes' preconcentration.…”

Section: Introductionmentioning

confidence: 99%

Application of curcumin as a green and new sorbent in deep eutectic solvent‐based dispersive micro‐solid phase extraction of several polycyclic aromatic hydrocarbons from honey samples prior to gas chromatography–mass spectrometry determination

Mogaddam

Jouyban

Nemati

et al. 2021

J of Separation Science

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A green, simple, and efficient dispersive micro-solid phase extraction method was developed for the extraction of polycyclic aromatic hydrocarbons from honey samples. In this method, for the first time, curcumin was used as an efficient and green sorbent to extract the analytes from the sample. After that the adsorbed analytes were eluted using a deep eutectic solvent prepared by mixing tetrabutylammonium chloride: ethylene glycol and analyzed by gas chromatographymass spectrometry. Important experimental factors affecting adsorption and desorption steps of the method were optimized and under optimal experimental conditions, low limits of detection (0.14-0.37 ng/g) and quantification (0.49-1.3 ng/g), wide linear range (1.3-500 ng/g) with a coefficient of determination ≥0.994 were obtained. Relative standard deviation values for intra-and interday precisions were ≤7.5% for all of the analytes at a concentration of 2 ng/g for each analyte (n = 6). Extraction recovery of the method was in the range of 72-81%. Finally, 20 honey samples were analyzed and the analytes were successfully detected. The method is environment friendly because of the use of curcumin as a sorbent. Also, biodegradability of the used deep eutectic solvent components is another advantage of the method.

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Single-use porous thin film extraction with gas chromatography atmospheric pressure chemical ionization tandem mass spectrometry for high-throughput analysis of 16 PAHs

Cited by 30 publications

References 29 publications

Development and application of a thin‐film molecularly imprinted polymer for the measurement of mycophenolic acid in human plasma

Development and application of a thin‐film molecularly imprinted polymer for the measurement of mycophenolic acid in human plasma

Smokeless tobacco analysis: Simultaneous extraction and purification of alkaloids, volatile N‐nitrosamines, and polycyclic hydrocarbons for GC‐MS/MS

Application of curcumin as a green and new sorbent in deep eutectic solvent‐based dispersive micro‐solid phase extraction of several polycyclic aromatic hydrocarbons from honey samples prior to gas chromatography–mass spectrometry determination

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