Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12

Lindblow-Kull, Carole; Kull, F. Jon; Shrift, Alex

doi:10.1128/jb.163.3.1267-1269.1985

Cited by 60 publications

(25 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This SO 4 concentration is 16 times higher than the Se (VI) concentration, which suggests a higher affinity of algal membrane transporters for Se(VI) than for SO 4 . This result contrasts with those obtained for the bacterium Escherichia coli, which has a higher affinity for SO 4 compared to Se(VI) (Lindblow-Kull et al 1985;Aguilar-Barajas et al 2011). Chlamydomonas reinhardtii [Se] continued to decrease up to the highest SO 4 treatments (500 compared to 2000 and 4000 mM; inset in Figure 3A).…”

Section: Se Concentrations In Natural Planktonic Food Chainscontrasting

confidence: 95%

Organic selenium, selenate, and selenite accumulation by lake plankton and the alga Chlamydomonas reinhardtii at different pH and sulfate concentrations

Ponton

Fortin

Hare

2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Selenium (Se) concentrations measured in lake planktonic food chains (microplankton <64 μm, copepods, and Chaoborus larvae) were strongly correlated with the concentrations of dissolved organic Se. These correlations were strengthened slightly by adding the concentrations of dissolved selenate to those of organic Se. To better understand the role of Se species and the influence of water chemistry on Se uptake, we exposed the green alga Chlamydomonas reinhardtii to selenite, selenate, or selenomethionine at various H ion and sulfate concentrations under controlled laboratory conditions. At low sulfate concentrations, inorganic Se species (selenate >> selenite) were more readily accumulated by this alga than was selenomethionine. However, at higher sulfate concentrations the uptake of selenite was higher than that of selenate, whereas the uptake of selenomethionine remained unchanged. Although the pH of the exposure water did not influence the uptake of selenate by this alga, the accumulation of selenomethionine and selenite increased with pH because of their relative pH-related speciation. The Se concentrations that we measured in C. reinhardtii exposed to selenomethionine were 30 times lower than those that we measured in field-collected microplankton exposed in the same laboratory conditions. This difference is explained by the taxa present in the microplankton samples. Using the present laboratory measurements of Se uptake in microplankton and of natural Se concentrations in lake water allowed us to model Se concentrations in a lake pelagic food chain. Environ Toxicol Chem 2018;37:2112-2122. © 2018 SETAC.

show abstract

Section: Se Concentrations In Natural Planktonic Food Chainscontrasting

confidence: 95%

Organic selenium, selenate, and selenite accumulation by lake plankton and the alga Chlamydomonas reinhardtii at different pH and sulfate concentrations

Ponton

Fortin

Hare

2018

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…S1). For selenium ion resistance, we found genes corresponding to the selenium ion transporters DedA (75), TsgA (76), and putative selenium ion and sulfate importer CysA (77). For cadmium ion resistance, we found the genes CzcABC, which encode the components of a cation transporter (78), its response regulator CzcD (79), and a cadmium-responsive transcriptional regulator, CadR (80).…”

Section: Resultsmentioning

confidence: 99%

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

Rothman

Russell

Leger

et al. 2020

Preprint

View full text Add to dashboard Cite

Bumble bees (Bombus spp.) are important and widespread insect pollinators, but the act of foraging on flowers can expose them to harmful pesticides and environmental chemicals such as oxidizers and heavy metals. How these compounds directly influence bee survival and indirectly affect bee health via the gut microbiome is largely unknown. As the toxins and toxicants in floral nectar and pollen take many forms, we explored the genomes of core bumblebee microbes (Using RAST) for their potential to detoxify cadmium, copper, selenium, the neonicotinoid pesticide imidacloprid, and hydrogen peroxide - which have all been identified in floral nectar and pollen. We then exposed Bombus impatiens workers to varying concentrations of these chemicals spiked into their diet and identified the direct effects on bee survival. Using field realistic doses, we further explored indirect effects on bee microbiomes. We found multiple genes in core gut microbes that have the potential to aid in detoxifying harmful chemicals. We also found that while the chemicals are largely toxic at levels within and above field-realistic concentrations, the field-realistic concentrations - except for imidacloprid - altered the composition of the bee microbiome, potentially causing gut dysbiosis. Overall, our study shows that environmental chemicals found in floral nectar and pollen can directly cause bee mortality, and at field-realistic levels, likely have indirect, deleterious effects on bee health via their influence on the bee microbiome.

show abstract

“…Many of the non-bumble bee-specific taxa had at least some active selenoproteins (Supporting Information Table ST1). Each of the other core bumble bee gut bacteria or non-core bacteria that were differentially abundant in our study had putative genes corresponding to the sulfate and thiosulfate import ATP-binding protein CysA (Lindblow-Kull et al, 1985), the putative (Guzzo and Dubow, 2000) selenite-inducible transporter TsgA (Guzzo and Dubow, 2000), the selenate/selenite transporter DedA (Ledgham et al, 2005), L-seryl-tRNA(Sec) selenium transferase SelA Fig. 1.…”

Section: Genes Involved In Selenium Ion Uptake and Processingmentioning

confidence: 87%

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

Rothman

Leger

Graystock

et al. 2019

Environmental Microbiology

View full text Add to dashboard Cite

Summary Bumble bees are important and widespread insect pollinators who face many environmental challenges. For example, bees are exposed to the metalloid selenate when foraging on pollen and nectar from plants growing in contaminated soils. As it has been shown that the microbiome of animals reduces metalloid toxicity, we assayed the ability of the bee microbiome to increase survivorship against selenate challenge. We exposed uninoculated or microbiota‐inoculated Bombus impatiens workers to a field‐realistic dose of 0.75 mg l−1 selenate and found that microbiota‐inoculated bees survive slightly but significantly longer than uninoculated bees. Using 16S rRNA gene sequencing, we found that selenate exposure altered gut microbial community composition and relative abundance of specific core bacteria. We also grew two core bumble bee microbes – Snodgrassella alvi and Lactobacillus bombicola – in selenate‐spiked media and found that these bacteria grew in the tested concentrations of 0.001–10 mg l−1 selenate. Furthermore, the genomes of these microbes harbour genes involved in selenate detoxification. The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate, but the specific mechanisms and colony‐level benefits under natural settings require further study.

show abstract

Single transporter for sulfate, selenate, and selenite in Escherichia coli K-12

Cited by 60 publications

References 12 publications

Organic selenium, selenate, and selenite accumulation by lake plankton and the alga Chlamydomonas reinhardtii at different pH and sulfate concentrations

Organic selenium, selenate, and selenite accumulation by lake plankton and the alga Chlamydomonas reinhardtii at different pH and sulfate concentrations

The direct and indirect effects of environmental toxicants on the health of bumble bees and their microbiomes

The bumble bee microbiome increases survival of bees exposed to selenate toxicity

Contact Info

Product

Resources

About