Single-sweep polarographic investigation of concanavalin A and its interaction with polysaccharide

Liu, Jie; Zeng, Baizhao; Lu, Xiandan; Zhou, Xingyao; Zhao, Zhijun

doi:10.1016/0003-2697(91)90320-s

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Since only specificallybound stains fluoresced, excess stain was not rinsed out (Holloway and Cowen 1996). Concanavalin A-fluorescence isothycyanate (Con A) was used at a final concentration of 5 pg ml 1 to identify polysaccharides containing e-D-mannopyranosyl or e-Dglucopyronsyl residues (Liu et al 1991), and, with less intense fluorescence, galactose (Holloway and Cowen 1996). Propidium iodide (PI) was used at a final concentration of 4 pgml-1 to identify senescent and dead ceils and extracellular DNA.…”

Section: Sample Collectionmentioning

confidence: 99%

Structural and chemical analysis of marine aggragates: in situ macrophotography and laser confocal and electron microscopy

Cowen

Holloway

1996

Marine Biology

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Marine-snow aggregates are compositionally diverse macroparticles that dominate the vertical material flux in many open-ocean environments. There is little documentation of the detailed physical structure and microcomposition of marine-snow aggregates, yet such characteristics both influence and are a function of aggregation mechanisms. This paper describes the application of in situ macrophotography followed by laser-scanning confocal microscopy (LSCM) as a means of observing the fine structure of delicate marine-snow aggregates in their fully hydrated state. Use of specific fluorescent stains yielded microcompositional images, and addition of analytical scanning electron microscopy allowed analysis of subcomponent particles within the aggregates. Application of these techniques to structurally diverse aggregates collected from the oligotrophic North Pacific surface waters revealed large variations in structure, density, relative homogeneity, extracellular matrix material, abundance and type of phytoplankton, and organic and mineral microcomposition.

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Section: Sample Collectionmentioning

confidence: 99%