Single-stranded DNA from the parvovirus, H-1

The nucleotide sequence of the 5' terminus of the parvovirus H-1 was determined. There are two orientations of the 242-base-pair terminal palindrome in native replicative form DNA, one inverted with respect to the other. Adjacent to the terminal palindrome is an AT-rich region that is noncoding and contains a 55-base-pair tandem repeat. The addition mutant of H-1, DI-1, was also sequenced in this region and shown to have three copies of the tandem repeat sequence. Similarly, the related parvovirus H-3 contains only one copy of this repeat sequence. This region contains the replication origin for parvovirus replicative form DNA replication. Some of the implications of these results are discussed.

mentioning

confidence: 99%

DNA sequence of the 5' terminus containing the replication origin of parvovirus replicative form DNA

Rhode¹,

Klaassen²

1982

“…The H-1 virus, a member of the family of autonomous, nondefective parvoviruses, is a small, icosahedral virus which infects eucaryotic cells (11). The single-stranded DNA genome of the H-1 virus is 1.6 x 106 daltons (12,16) and is encapsidated by three viral proteins designated VPi, VP2', and VP2 (alternatively called A, B, and C polypeptides, respectively) (4). The virus particles isolated from parvovirus-infected cells have some very intriguing characteristics with respect to their protein components and their density in CsCl.…”

mentioning

confidence: 99%

Infectious process of the parvovirus H-1: correlation of protein content, particle density, and viral infectivity

Paradiso¹

1981

The infectious particles of the parvovirus H-1 were characterized with respect to protein content, density in CsCl, and specific infectivity. Heavy-full and lightfull particles were purified from infected simian virus 40-transformed newborn human kidney (NB) cells and from simian virus 40-transformed hamster kidney (THK) cells. Analysis of the protein content of these particles demonstrated that the ratio of viral protein VP2' to VP2 was the same in heavy-full and light-full 800 on July 7, 2020 by guest

“…The minute virus of mice (MVM) (5) is a member of the parvovirus group of small, isometric viruses containing 1.2 x 10 to 2.0 x 101 daltons of single-stranded DNA (6,17,19,20,24,32). These viruses fall into two subgroups, the adenovirus associated viruses (AAV) which are dependent upon adenovirus co-infection and those which are autonomous in most cell systems.…”

mentioning

confidence: 99%

Replication of the Parvovirus MVM II. Isolation and Characterization of Intermediates in the Replication of the Viral Deoxyribonucleic Acid

Tattersall

Crawford

Shatkin

1973

The time course of the appearance of intracellular viral DNA has been studied in mouse L cells infected with the single-stranded DNA virus MVM (minute virus of mice) by using a selective extraction procedure. Approximately half of this DNA elutes from hydroxyapatite as single-stranded DNA. It is sensitive to Escherichia coli exonuclease I and shows a sedimentation profile similar to DNA from the virus, suggesting that it is progeny viral DNA. The remainder of the selectively extracted DNA elutes from hydroxyapatite in the position of double-stranded DNA and is resistant to exonuclease I. Most of this DNA has a sedimentation coefficient of 14 to 16S, indicating that its molecular weight is twice that of the viral DNA. Denaturation renders the majority of the double-stranded DNA sensitive to exonuclease I, but a significant fraction renatures spontaneously in a monomolecular fashion, indicating that it has a cross-linked or hairpin structure. Chromatography of the double-stranded DNA on benzoylated diethylaminoethyl cellulose resolves two components, one with duplex structure and one which contains single-stranded regions. A short pulse label late in infection predominantly labels the latter class of DNA, suggesting that it contains replicating intermediates. The possible roles of these various forms of DNA in the replication of the viral genome are discussed.