Single‐stranded DNA binding factor AtWHY1 modulates telomere length homeostasis in Arabidopsis

Yoo, Hyera; Kwon, Chian; Lee, Myeong Min; Chung, In Kwon

doi:10.1111/j.1365-313x.2006.02974.x

Cited by 80 publications

(82 citation statements)

References 43 publications

(56 reference statements)

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“…Until now, only the PR1 gene has been proven to be downregulated as a result of a TILLING mutation in WHY1 in Arabidopsis (Desveaux et al, 2004(Desveaux et al, , 2005. Besides the activity as a transcriptional activator, AtWHY1 was also identified in a fraction of telomere-binding proteins, and its knockout mutant appeared to have a shorter telomere (Yoo et al, 2007). This result led the authors to suggest a role for WHY1 in telomerase inhibition (Yoo et al, 2007).…”

mentioning

confidence: 87%

“…Besides the activity as a transcriptional activator, AtWHY1 was also identified in a fraction of telomere-binding proteins, and its knockout mutant appeared to have a shorter telomere (Yoo et al, 2007). This result led the authors to suggest a role for WHY1 in telomerase inhibition (Yoo et al, 2007). In addition, WHY1 also functions as a repressor in the salicylic acid-mediated pathogen-responsive pathway by binding to the GAGAAATT motif of the kinesin AtKP1 promoter (Xiong et al, 2009).…”

mentioning

confidence: 91%

“…The DNA-binding motifs recognized by WHY1 have been well characterized by others (Després et al, 1995;Desveaux et al, 2000Desveaux et al, , 2002Yoo et al, 2007;Xiong et al, 2009). We used a ChIP assay to screen downstream targets of WHY1.…”

Section: The Why1 Protein Binds To the Promoter Of Wrky53mentioning

confidence: 99%

“…The promoter of the WRKY53 gene contains a sequence stretch with high similarity to both the ERE motif and the reverse telomere-like sequence AAATCCC or "AAAT region" (Desveaux et al, 2000;Yoo et al, 2007;Fig. 3A) that implies a direct interaction between WHY1 and the promoter of WRKY53.…”

Section: The Why1 Protein Binds To the Promoter Of Wrky53mentioning

confidence: 99%

“…8A). According to the ChIP-sequencing results (Supplemental Table S3) and based on previous reports that WHY1 could bind to the telomere sequence in the promoter of PR1 and that WHY1 had functions in the DNA repair of double-strand breaks (DSB; Desveaux et al, 2004;Yoo et al, 2007;Maréchal et al, 2009;Maréchal and Brisson, 2010), the PR1 promoter was selected as a positive candidate control. As such, WRKY53, WRKY33, and SPO11 were detected in each DNA sample (Fig.…”

Section: Why1 Could Not Change the Delayed-senescence Phenotype Of Thmentioning

confidence: 99%

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The Single-Stranded DNA-Binding Protein WHIRLY1 Represses WRKY53 Expression and Delays Leaf Senescence in a Developmental Stage-Dependent Manner in Arabidopsis

et al. 2013

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Leaf senescence in plants involves both positive and negative transcriptional regulation. In this work, we show evidence for the single-stranded DNA-binding protein WHIRLY1 (WHY1) that functions as an upstream suppressor of WRKY53 in a developmental stage-dependent manner during leaf senescence in Arabidopsis (Arabidopsis thaliana). The why1 mutant displayed an early-senescence phenotype. In this background, the expression levels of both WRKY53 and the senescenceassociated protease gene SAG12 increased. WHY1 bound to the sequence region that contains an elicitor response element motif-like sequence, GNNNAAATT, plus an AT-rich telomeric repeat-like sequence in the WRKY53 promoter in in vivo and in vitro mutagenesis assays as well as in a chromatin immunoprecipitation assay. This binding to the promoter of WRKY53 was regulated in a developmental stage-dependent manner, as verified by chromatin immunoprecipitation-polymerase chain reaction assay. This direct interaction was further determined by a transient expression assay in which WHY1 repressed b-GLUCURONIDASE gene expression driven by the WRKY53 promoter. Genetic analysis of double mutant transgenic plants revealed that WHY1 overexpression in the wrky53 mutant (oeWHY1wrky53) had no effect on the stay-green phenotype of the wrky53 mutant, while a WHY1 knockout mutant in the wrky53 mutant background (why1wrky53) generated subtle change in the leaf yellow/green phenotype. These results suggest that WHY1 was an upstream regulator of WRKY53 during leaf senescence.

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confidence: 87%

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confidence: 91%

Section: The Why1 Protein Binds To the Promoter Of Wrky53mentioning

confidence: 99%

Section: The Why1 Protein Binds To the Promoter Of Wrky53mentioning

confidence: 99%

Section: Why1 Could Not Change the Delayed-senescence Phenotype Of Thmentioning

confidence: 99%

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The Single-Stranded DNA-Binding Protein WHIRLY1 Represses WRKY53 Expression and Delays Leaf Senescence in a Developmental Stage-Dependent Manner in Arabidopsis

et al. 2013

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Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

Yoo

Han

Chen

2020

Annual Plant Reviews Online

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The biogenesis of photosynthetically active chloroplasts in flowering plants (angiosperms) is initiated by light through the red and far‐red photoreceptors, phytochromes, which activate photosynthesis‐associated genes encoded by both the nuclear and plastid genomes. Because photoactivated phytochromes localize to the nucleus but not the plastids, phytochromes ought to control plastid transcription through nucleus‐to‐plastid or anterograde signalling. However, the mechanism of anterograde signalling remains poorly understood. Recent advances in our understanding of phytochrome signalling and plastid transcription led to the discovery of the framework of an anterograde signalling pathway, which links light‐dependent regulation of nuclear repressors of chloroplast biogenesis to the control of the assembly and activity of the bacterial‐type plastidial RNA polymerase for transcribing plastid‐encoded photosynthesis genes. This article is aimed to gather and reanalyze the evidence in the literature relevant to the phytochrome control of chloroplast biogenesis to forge a model of anterograde signalling mechanisms for the regulation of plastid transcription by light.

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WHIRLY protein functions in plants

Taylor

West

Foyer

2022

Food and Energy Security

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Environmental stresses pose a significant threat to food security. Understanding the function of proteins that regulate plant responses to biotic and abiotic stresses is therefore pivotal in developing strategies for crop improvement. The WHIRLY (WHY) family of DNA‐binding proteins are important in this regard because they fulfil a portfolio of important functions in organelles and nuclei. The WHY1 and WHY2 proteins function as transcription factors in the nucleus regulating phytohormone synthesis and associated growth and stress responses, as well as fulfilling crucial roles in DNA and RNA metabolism in plastids and mitochondria. WHY1, WHY2 (and WHY3 proteins in Arabidopsis) maintain organelle genome stability and serve as auxiliary factors for homologous recombination and double‐strand break repair. Our understanding of WHY protein functions has greatly increased in recent years, as has our knowledge of the flexibility of their localization and overlap of functions but there is no review of the topic in the literature. Our aim in this review was therefore to provide a comprehensive overview of the topic, discussing WHY protein functions in nuclei and organelles and highlighting roles in plant development and stress responses. In particular, we consider areas of uncertainty such as the flexible localization of WHY proteins in terms of retrograde signalling connecting mitochondria, plastids, and the nucleus. Moreover, we identify WHY proteins as important targets in plant breeding programmes designed to increase stress tolerance and the sustainability of crop yield in a changing climate.

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Single‐stranded DNA binding factor AtWHY1 modulates telomere length homeostasis in Arabidopsis

Cited by 80 publications

References 43 publications

The Single-Stranded DNA-Binding Protein WHIRLY1 Represses WRKY53 Expression and Delays Leaf Senescence in a Developmental Stage-Dependent Manner in Arabidopsis

The Single-Stranded DNA-Binding Protein WHIRLY1 Represses WRKY53 Expression and Delays Leaf Senescence in a Developmental Stage-Dependent Manner in Arabidopsis

Nucleus‐to‐Plastid Phytochrome Signalling in Controlling Chloroplast Biogenesis

WHIRLY protein functions in plants

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