Single-strand-conformation polymorphism of ribosomal DNA for rapid species differentiation in genus Phytophthora

Kong, Ping; Hong, Chuanxue; Richardson, Patricia A.; Gallegly, M. E.

doi:10.1016/s1087-1845(03)00052-5

Cited by 83 publications

(83 citation statements)

References 40 publications

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“…Also, the way how some traditional and well-known taxa are viewed has substantially changed. For instance, recent studies have revealed a high DNA variability in Phytophthora citricola sensu lato suggesting the distinction of several lineages (Kong et al 2003;Bhat & Browne 2007;Gallegly & Hong 2008;Moralejo et al 2008). Based on SSCP fingerprinting and isozyme analysis results, the original species were divided into distinct subgroups (Oudemans et al 1994;Kong et al 2003;Gallegly & Hong 2008).…”

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confidence: 99%

“…For instance, recent studies have revealed a high DNA variability in Phytophthora citricola sensu lato suggesting the distinction of several lineages (Kong et al 2003;Bhat & Browne 2007;Gallegly & Hong 2008;Moralejo et al 2008). Based on SSCP fingerprinting and isozyme analysis results, the original species were divided into distinct subgroups (Oudemans et al 1994;Kong et al 2003;Gallegly & Hong 2008). Further, DNA sequence analyses based on the ITS region of the rRNA operon, mitochondrial cox1 and β-tubulin gene regions demonstrated that the P. citricola complex comprised four lineages corresponding to P. citricola sensu stricto, P. citricola I, and two novel species -P. multivora and P. plurivora -that partially differed in their morphology, physiology, and geography, too .…”

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Occurrence of Phytophthora multivora and Phytophthora plurivora in the Czech Republic

Mrazkova¹,

Černý²,

Tomšovský³

et al. 2013

Plant Prot. Sci.

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Mrázková M., Černý K., Tomšovský M., Strnadová V., Gregorová B., Holub V., Pánek M., Havrdová L., Hejná M. (2013): Occurrence of Phytophthora multivora and Phytophthora plurivora in the Czech Republic. Plant Protect. Sci., 49: 155-164.Beginning in 2006, a survey of two related Phytophthora species, P. multivora and P. plurivora, was performed in the Czech Republic. Both pathogens were distributed throughout a broad range of environments including forest and riparian stands and probably became naturalised in the country. The two species differed in their frequency and elevational distribution. P. multivora was less frequent, but commonly occurred in the lowest regions such as Central Bohemia and South Moravia, i.e. areas which generally exhibit a high level of invasion. This species was isolated primarily from Quercus robur and found to be involved in oak decline. Moreover it poses a high risk to other forest trees. P. plurivora was distributed in a broad range of elevations over the entire area. A substrate specificity was detected in P. plurivora -the isolates from forest trees were more aggressive to such trees than the isolates from ericaceous ornamental plants.

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confidence: 99%

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confidence: 99%

Occurrence of Phytophthora multivora and Phytophthora plurivora in the Czech Republic

Mrazkova¹,

Černý²,

Tomšovský³

et al. 2013

Plant Prot. Sci.

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Section: Phytophthora Ramorum a New Invader Causing Severe Problems mentioning

confidence: 99%

“…Previous attempts to unravel the taxonomy of this complex include isozyme analysis and single-strandconformation polymorphism of ribosomal DNA (SSCP) fingerprinting techniques (Oudemans et al 1994, Kong et al 2003. Both genetic analyses revealed several distinct subgroups, although in most cases no differences in morphology or host range were detected (Oudemans et al 1994, Kong et al 2003. Two molecular phylogenetic studies of the internal transcribed regions (ITS1 and ITS2) and 5.8S gene of the rRNA operon, the mitochondrial cox1 and β-tubulin genes revealed two new species in the P. citricola complex (Scott et al 2009, Jung and.…”

Section: Phytophthora Ramorum a New Invader Causing Severe Problems mentioning

confidence: 99%

Introduced pathogens found on ornamentals, strawberry and trees in Finland over the past 20 years

Lilja

Rytkönen²,

Hantula³

2008

AFSci

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The comparative ease and speed of international trade and travel have enabled or enhanced the spread of pests around the globe. For example, trade in ornamental plants has bolstered the spread of alien Oomycetes such as pathogenic species of Phytophthora. To date, four Phytophthora species have been identified in Finland: P. cactorum on Fragaria x ananassa, Betula pendula and Rhododendron spp., P. plurivora on Rhododendron spp. and Syringa vulgaris, and P. pini and P. ramorum on Rhododendron spp. The ascomycete Colletotrichum acutatum, which was listed as a quarantine pathogen by the European Union until 2009, was introduced in 2000 and can survive in plant debris over two winters in Finland. Positive PCR results have also been obtained from bait plants grown in soil collected from locations where diseased Fragaria x ananassa plants had earlier been destroyed. In the mid-1990s, there was an epidemic of foliar rust caused by the Asian basidiomycete Melampsoridium hiratsukanum on Alnus glutinosa and A. incana. Recently, two ascomycetes that have been introduced are Dothistroma septosporum (responsible for red band needle blight on Pinus sylvestris) and Chalara fraxinea (causing ash decline on Fraxinus excelsior).

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“…The elucidation of the infraspecific structure of P. citricola (e.g. Kong et al 2003;Gallegly & Hong 2008) has resulted in the description of two new species, i.e. P. multivora and P. plurivora Scott et al 2009).…”

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confidence: 99%

Phytophthora plurivora T. Jung & T. I. Burgess and other Phytophthora species causing important diseases of ericaceous plants in the Czech Republic

Mrazkova¹,

Černý²,

Tomšovský³

et al. 2011

Plant Prot. Sci.

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Ornamental nurseries, garden centres, public gardens and urban greenery in the Czech Republic were surveyed in 2006-2009 for the presence of Phytophthora spp. and the diseases they cause on ericaceous plants. Diseased plants such as rhododendron spp., Pieris floribunda, Vaccinium sp., and Azalea sp. showed various symptoms including leaf spot, shoot blight, twig lesions or stem, root and collar rot. Nearly 140 Phytophthora isolates were collected from symptomatic plants in different areas of the country. Of the Phytophthora spp. on ericaceous plants or in their surroundings, P. plurivora appeared to be the most common species. Herein, we focus on the most frequently occurring species, P. plurivora, and describe its morpho-physiological and pathogenicity features and confirm its identity based on ITS sequences of rDNA. In addition, we give a list of other Phytophthora spp. including P. cactorum, P. cambivora, P. cinnamomi, P. citrophthora, P. megasperma, P. multivora, P. ramorum, and P. gonapodyides that we identified on the basis of their cultural and morphological characteristics and DNA sequences. We also discuss their importance in cultivated and natural ecosystems.

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Single-strand-conformation polymorphism of ribosomal DNA for rapid species differentiation in genus Phytophthora

Cited by 83 publications

References 40 publications

Occurrence of Phytophthora multivora and Phytophthora plurivora in the Czech Republic

Occurrence of Phytophthora multivora and Phytophthora plurivora in the Czech Republic

Introduced pathogens found on ornamentals, strawberry and trees in Finland over the past 20 years

Phytophthora plurivora T. Jung & T. I. Burgess and other Phytophthora species causing important diseases of ericaceous plants in the Czech Republic

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