Single strand breaks and mutagenesis in yeast induced by photodynamic treatment with chloroaluminum phthalocyanine

Paardekooper, Michel; Bruijne, Adriaan W. De; Gompel, A. E. Van; Verhage, Richard A.; Averbeck, D.; Dubbelman, T. M. A. R.; Broek, Peter J.A. Van den

doi:10.1016/s1011-1344(97)00038-9

Cited by 14 publications

(10 citation statements)

References 37 publications

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“…The efficacy of antifungal PDT with Pc is strongly influenced by the type of Pc, solvent, and delivery system (33)(34)(35)(36). The ClAlPc used in the current study is insoluble in water and it was formulated in an o ⁄ w nanoemulsion.…”

Section: Discussionmentioning

confidence: 99%

“…PDT with ClAlPc led to several forms of cellular damage, such as potassium leakage, inhibition of enzymes involved in energy metabolism and protein synthesis but only inhibition of protein synthesis was considered a critical factor contributing to the loss of clonogenicity (35). PDT also resulted in DNA damage in the yeast cells although this is not the main determinant for cell killing (36).…”

Section: Discussionmentioning

confidence: 99%

“…Phthalocyanines (Pc) are among second generation PS more promising for use in PDT due to their intense absorption in the red region of visible light, nontoxicity, selective localization in tumors and efficient generation of singlet oxygen (30)(31)(32). Additionally, it has been reported that different types of Pc have the potential to photoinactivate yeast cells (33)(34)(35)(36)(37)(38)(39). Structurally, a wide range of Pc is available, for example with different central metals and ⁄ or different degrees of sulfonation.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Photodynamic Inactivation of Cryptococcus neoformans Melanized Cells with Chloroaluminum Phthalocyanine Nanoemulsion

Rodrigues

Primo

Tedesco

et al. 2012

Photochem & Photobiology

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The selection of fungi resistant to currently used fungicides and the emergence of new pathogenic species make the development of alternative fungus-control techniques highly desirable. Photodynamic antimicrobial chemotherapy (PACT) is a promising method which combines a nontoxic photosensitizer (PS) with visible light to cause selective killing of microbial cells. The development of PACT to treat mycoses or kill fungi in the environment depends on identifying effective PS for the different pathogenic species and delivery systems able to expand and optimize their use. In the present study, the in vitro susceptibility of Cryptococcus neoformans melanized cells to the photodynamic effects of the PS agent ClAlPc in nanoemulsion (ClAlPc/NE) was examined. Cells were killed in a PS concentration- and light dose-dependent manner. Treatment with ClAlPc/NE, using PS concentrations (e.g. 4.5 μm) and light doses (e.g. 10 J cm(-2)) compatible with PACT, resulted in a reduction of up to 6 logs in survival. Washing the cells to remove unbound PS before light exposure did not inhibit fungal photodynamic inactivation. Internalization of ClAlPc by C. neoformans was confirmed by confocal fluorescence microscopy, and the degree of uptake was dependent on PS concentration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Photodynamic Inactivation of Cryptococcus neoformans Melanized Cells with Chloroaluminum Phthalocyanine Nanoemulsion

Rodrigues

Primo

Tedesco

et al. 2012

Photochem & Photobiology

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“…In order to optimize the PDI of microorganisms, knowledge of the underlying mechanisms is indispensable. However, in the majority of studies investigating the mechanisms of yeast PDI, the anionic hematoporphyrin and its derivatives (3,39), the hydrophobic chloroaluminum phthalocyanine (29,32) or the cationic thiazine dye toluidine blue (6,13,28,30), are used as photosensitizers.…”

mentioning

confidence: 99%

Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Lambrechts¹,

Aalders²,

Marle

2005

Antimicrob Agents Chemother

157

126

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“…Single strand DNA breaks and mutagenicity induced by photodynamic action of aluminum phthalocyanine have been detected in yeast [31]. In mammalian cells frequency of mutations induced by AlPc-PDT has been shown to be dependent on the p53 status and cellular repair capacities[32].…”

Section: Resultsmentioning

confidence: 99%

Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line

et al. 2010

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BackgroundPhotodynamic therapy (PDT) involves excitation of sensitizer molecules by visible light in the presence of molecular oxygen, thereby generating reactive oxygen species (ROS) through electron/energy transfer processes. The ROS, thus produced can cause damage to both the structure and the function of the cellular constituents resulting in cell death. Our preliminary investigations of dose-response relationships in a human glioma cell line (BMG-1) showed that disulphonated aluminum phthalocyanine (AlPcS2) photodynamically induced loss of cell survival in a concentration dependent manner up to 1 μM, further increases in AlPcS2concentration (>1 μM) were, however, observed to decrease the photodynamic toxicity. Considering the fact that for most photosensitizers only monotonic dose-response (survival) relationships have been reported, this result was unexpected. The present studies were, therefore, undertaken to further investigate the concentration dependent photodynamic effects of AlPcS2.MethodsConcentration-dependent cellular uptake, sub-cellular localization, proliferation and photodynamic effects of AlPcS2 were investigated in BMG-1 cells by absorbance and fluorescence measurements, image analysis, cell counting and colony forming assays, flow cytometry and micronuclei formation respectively.ResultsThe cellular uptake as a function of extra-cellular AlPcS2 concentrations was observed to be biphasic. AlPcS2 was distributed throughout the cytoplasm with intense fluorescence in the perinuclear regions at a concentration of 1 μM, while a weak diffuse fluorescence was observed at higher concentrations. A concentration-dependent decrease in cell proliferation with accumulation of cells in G2+M phase was observed after PDT. The response of clonogenic survival after AlPcS2-PDT was non-monotonic with respect to AlPcS2 concentration.ConclusionsBased on the results we conclude that concentration-dependent changes in physico-chemical properties of sensitizer such as aggregation may influence intracellular transport and localization of photosensitizer. Consequent modifications in the photodynamic induction of lesions and their repair leading to different modes of cell death may contribute to the observed non-linear effects.

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Single strand breaks and mutagenesis in yeast induced by photodynamic treatment with chloroaluminum phthalocyanine

Cited by 14 publications

References 37 publications

In Vitro Photodynamic Inactivation of Cryptococcus neoformans Melanized Cells with Chloroaluminum Phthalocyanine Nanoemulsion

In Vitro Photodynamic Inactivation of Cryptococcus neoformans Melanized Cells with Chloroaluminum Phthalocyanine Nanoemulsion

Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Non-monotonic changes in clonogenic cell survival induced by disulphonated aluminum phthalocyanine photodynamic treatment in a human glioma cell line

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