Single-step, wash-free digital immunoassay for rapid quantitative analysis of serological antibody against SARS-CoV-2 by photonic resonator absorption microscopy

Zhao, Bin; Che, Congnyu; Wang, Weijing; Li, Nantao; Cunningham, Brian T.

doi:10.1016/j.talanta.2020.122004

Cited by 44 publications

(48 citation statements)

References 45 publications

(38 reference statements)

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“…Finally, a photonic resonator absorption microscopy (PRAM) method was used for imaging. The limit of quantification and detection of this method was 32.0 ± 8.9 pg/mL and 26.7 ± 7.7 pg/mL, respectively [54].…”

Section: Plasmonic Biosensorsmentioning

confidence: 86%

“…For dissolving the immunoreagents, both bovine serum albumin (BSA) and a phosphate buffer solution (PBS) were used. To enhance the binding of biomarkers to the sensor surface, functionalize [47], b naked-eye detection of SARS-CoV-2 by ASO capped gold nanoparticles [49], c PRAM-based AC + DC immunoassay [54] AuNPs were dispersed on the biosensor's surface. The ultratight field confinement of toroidal metasensor makes it possible to achieve high sensitivity like the LSPR biosensors.…”

Section: Plasmonic Biosensorsmentioning

confidence: 99%

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Nanomaterials to tackle the COVID-19 pandemic

Pishva

Yüce

2021

emergent mater.

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The rapid worldwide spread of the COVID-19 pandemic, caused by the severe acute respiratory SARS-CoV-2, has created an urgent need for its diagnosis and treatment. As a result, many researchers have sought to find the most efficient and appropriate methods to detect and treat the SARS-CoV-2 virus over the past few months. Real-time reverse-transcriptase polymerase chain reaction (RT-PCR) testing is currently used as one of the most reliable methods to detect the new virus; however, this method is time-consuming, labor-intensive, and requires trained laboratory workers. Moreover, despite its high sensitivity and specificity, false negatives are reported, especially in non-nasopharyngeal swab samples that yield lower viral loads. Therefore, designing and employing faster and more reliable methods seems necessary. In recent years, many attempts have been made to fabricate various nanomaterial-based biosensors to detect viruses and bacteria in clinical samples. The use of nanomaterials plays a significant role in improving the performance of biosensors. Plasmonic biosensors, field-effect transistor (FET)-based biosensors, electrochemical biosensors, and reverse transcription loop-mediated isothermal amplification (RT-LAMP) methods are only some of the effective ways to detect viruses. However, to use these biosensors to detect the SARS-CoV-2 virus, modifications must be performed to increase sensitivity and speed of testing due to the rapidly spreading nature of SARS-CoV-2, which requires an early point of care detection and treatment for pandemic control. Several studies have been carried out to show the nanomaterial-based biosensors' performance and success in detecting the novel virus. The limit of detection, accuracy, selectivity, and detection speed are some vital features that should be considered during the design of the SARS-CoV-2 biosensors. This review summarizes various nanomaterials-based sensor platforms to detect the SARS-CoV-2, and their design, advantages, and limitations.

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Section: Plasmonic Biosensorsmentioning

confidence: 86%

Section: Plasmonic Biosensorsmentioning

confidence: 99%

Nanomaterials to tackle the COVID-19 pandemic

Pishva

Yüce

2021

emergent mater.

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“… 150 Notably, a technique called plasmonic resonator absorption microscopy has been proven for detection of human IgG against SARS-CoV-2 with LoD of 100 pg/mL. 151 A 1D PhC coated with TiO 2 acts as a narrow bandwidth resonant reflector at 625 nm when submerged in aqueous media. Formation of a sandwich immunocomplex of COVID-19 IgG bound to antibodies immobilized on an AuNP, with the COVID-19 IgG then binding to spike proteins immobilized on the PhC surface, increases light absorption when the surface plasmon resonant wavelength matched the PhC resonant wavelength.…”

Section: Virus Sensor Technologiesmentioning

confidence: 99%

“…Utilizing LED illumination and a 2D image sensor allows for portability of the device. 151 Such techniques for antibody detection will become increasingly important for testing vaccination efficacy.…”

Section: Virus Sensor Technologiesmentioning

confidence: 99%

SARS-CoV-2 Tests: Bridging the Gap between Laboratory Sensors and Clinical Applications

et al. 2021

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This review covers emerging biosensors for SARS-CoV-2 detection together with a review of the biochemical and clinical assays that are in use in hospitals and clinical laboratories. We discuss the gap in bridging the current practice of testing laboratories with nucleic acid amplification methods, and the robustness of assays the laboratories seek, and what emerging SARS-CoV-2 sensors have currently addressed in the literature. Together with the established nucleic acid and biochemical tests, we review emerging technology and antibody tests to determine the effectiveness of vaccines on individuals.

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“…Targeting rapid and sensitive COVID-19 serology testing at the point of care (POC), a variety of portable immunosensing platforms have been developed based on microfluidics and biosensor technologies (Fabiani et al, 2021;Kim et al, 2021a;Lee et al, 2021;Li et al, 2021;Raziq et al, 2021;Roda et al, 2021;Tan et al, 2020a;Torrente-Rodríguez et al, 2020;Yakoh et al, 2021;Zakashansky et al, 2021;Z. Zhang et al, 2021;Zhao et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

SPEEDS: A Portable Serological Testing Platform for Rapid Electrochemical Detection of SARS-CoV-2 Antibodies

Peng

Pan

et al. 2021

Preprint

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The COVID-19 pandemic has resulted in a worldwide health crisis. Rapid diagnosis, new therapeutics and effective vaccines will all be required to stop the spread of COVID-19. Quantitative evaluation of serum antibody levels against the SARS-CoV-2 virus provides a means of monitoring a patient's immune response to a natural viral infection or vaccination, as well as evidence of a prior infection. In this paper, a portable and low-cost electrochemical immunosensor is developed for the rapid and accurate quantification of SARS-CoV-2 serum antibodies. The immunosensor is capable of quantifying the concentrations of immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies against the SARS-CoV-2 spike protein in human serum. For IgG and IgM, it provides measurements in the range of 10.1 ng/mL—60 μg/mL and 1.64 ng/mL — 50 μg/mL, respectively, and both antibodies can be assayed in 13 min. We also developed device stabilization and storage strategies to achieve stable performance of the immunosensor within 24-week storage at room temperature. We evaluated the performance of the immunosensor using COVID-19 patient serum samples collected at different time points after symptom onset. The rapid and sensitive detection of IgG and IgM provided by our immunosensor fulfills the need of rapid COVID-19 serology testing for both point-of-care diagnosis and population immunity screening.

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Single-step, wash-free digital immunoassay for rapid quantitative analysis of serological antibody against SARS-CoV-2 by photonic resonator absorption microscopy

Cited by 44 publications

References 45 publications

Nanomaterials to tackle the COVID-19 pandemic

Nanomaterials to tackle the COVID-19 pandemic

SARS-CoV-2 Tests: Bridging the Gap between Laboratory Sensors and Clinical Applications

SPEEDS: A Portable Serological Testing Platform for Rapid Electrochemical Detection of SARS-CoV-2 Antibodies

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