Single step surface modification of highly stable magnetic nanoparticles for purification of His-tag proteins

Sahu, Sumanta Kumar; Chakrabarty, Arindam; Bhattacharya, Debasish; Ghosh, Sudip Kumar; Pramanik, Panchanan

doi:10.1007/s11051-010-0140-y

Cited by 70 publications

(38 citation statements)

References 25 publications

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“…Although this method is easily adaptable to any protein expression systems, it has some limitations, including the need for pretreatment to remove the cell debris and colloid contaminants, a relatively long operation time, and protein solubility. More recently, nanoparticles or nanorods have emerged as a promising nano-tool for the separation of target proteins [11][12][13]. For example, Au/Fe 3 O 4 nanoparticles with Ni 2+ -NTA were applied to the separation of His-tagged proteins [14,15].…”

Section: Introductionmentioning

confidence: 99%

Functionalized hollow silica nanospheres for His-tagged protein purification

Yin¹,

Wei²,

Zou³

et al. 2015

Sensors and Actuators B: Chemical

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Section: Introductionmentioning

confidence: 99%

Functionalized hollow silica nanospheres for His-tagged protein purification

Yin¹,

Wei²,

Zou³

et al. 2015

Sensors and Actuators B: Chemical

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“…[ [1][2][3][4][5][6][7] have produced great efforts to reach a greater knowledge in basic and applied fields. All applications of these MNPs require a good dispersion in aqueous medium, chemical stability in the environment where it will be used and high values of specific 3 surface area (S g ) (the last conditions is not necessary in magnetic hyperthermia applications).…”

Section: Introductionmentioning

confidence: 99%

Changes on structural and magnetic properties of maghemite nanoparticles during their coverage with MCM-41

Fellenz

Berti

Soldati

et al. 2015

Ceramics International

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Changes on structural and magnetic properties of maghemite nanoparticles during their coverage with MCM-41, Ceramics International, http://dx.doi.org/10.1016/j.ceramint. 2015.08.016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Mössbauer spectroscopy at 25 and -260 ºC and transmission electron microscopy were used to study the magnetic and structural changes of the initial maghemite nanoparticles produced during its coverage with the mesoporous silica structure. The presence of the mesoporous structure inhibits the expected transformation from maghemite to hematite when the system was calcined in air at 510 ºC. It was established that a sintering of the nanoparticles during the calcination step occurs, leading to a magnetization saturation value similar to that of the bulk compound. In order to evaluate the capability as adsorbent, the magnetic composite was subjected to a surface amino-functionalization and then tested for Cr(VI) and Cu(II) adsorption from water.

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“…[24][25][26][27][28][29][30] Sahu et al reported a surface modication with N-phosphonomethyl iminodiacetic acid that is capable of forming nickel complexes. [24][25][26][27][28][29][30] Sahu et al reported a surface modication with N-phosphonomethyl iminodiacetic acid that is capable of forming nickel complexes.…”

Section: Introductionmentioning

confidence: 99%