Single-step procedure for the modification of graphite electrode by composite layer based on polypyrrole, Prussian blue and glucose oxidase

Ramanavičius, Arūnas; Rekertaitė, Asta Inesė; Valiūnas, Raimondas; Valiūnienė, Aušra

doi:10.1016/j.snb.2016.08.142

Cited by 58 publications

(27 citation statements)

References 19 publications

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“…ferrocyanide is also possible and may be desirable when a less acidic pH is required to preserve the activity of enzymes or other biomolecules. [15] Chemical growth, on the other hand, may also be used to produce Prussian Blue films, both on electrodes [26,30,31] and on inert surfaces [32] in many different ways. [33] However, this is not an ideal approach because it lacks the surface selectivity and much of the control that electrochemical methods enjoy.…”

Section: Preparation Of Pbmentioning

confidence: 99%

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Pellitero

Fremeau

Villa

et al. 2019

Sensors and Actuators B: Chemical

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Highlights We present the production of an electrochromic screen-printable paste based on Prussian Blue. These blue electrodes display excellent spectroelectrochemical reversibility and high catalytic activity towards the reduction of H2O2.  These blue electrodes enable both the electrochemical and optical quantitative determination of hydrogen peroxide.  The reported blue electrodes are highly suitable transducers for the construction of oxidase-based biosensors.  A glucose biosensor is presented to demonstrate the spectroelectrochemical capabilities of these new electrodes. AbstractPrussian Blue (PB)-modified graphite screen-printed electrodes are increasingly being used in electrochemical biosensors. However, they do not allow the observation of the electrochromism of PB. This work presents the construction of PB-based, electrochromic screen-printed biosensors. Although electrically more resistive than their graphite counterparts, these new PBbased electrodes enable both the amperometric and colorimetric detection of hydrogen peroxide.This is the first time that this has been achieved using screen-printed electrodes, and we demonstrate it spectroelectrochemically on a glucose biosensor. The biosensor electrochemical performance equals that of previously reported PB/graphite electrodes, being able to detect down to 4 µM H2O2 and 54 µM glucose. At the same time, and in contrast to PB/graphite electrodes, the new PB-based electrodes afford the optical detection of these two analytes down to 1.2 µM and 15 µM, respectively. The dynamic ranges of the glucose biosensors obtained at the PBbased electrodes are 0.1-1 mM (amperometric) and 0.025-2.5 mM (Colorimetric), matching the physiological glucose concentration range in body fluids other than blood or serum.

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Section: Preparation Of Pbmentioning

confidence: 99%

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Pellitero

Fremeau

Villa

et al. 2019

Sensors and Actuators B: Chemical

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“…The ideal methodology should be to fabricate the bioelectrode in a single step electrodeposition, as explained by Ramanavicius et al. to estimate glucose. They have successfully deposited Prussian blue and glucose oxidase through one step electro‐polymerization.…”

Section: Introductionmentioning

confidence: 99%

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

2018

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A simple single‐step fabrication of a novel biocomposite electrode was used for cholesterol sensing through direct electron transfer. The bio‐composite was prepared by polypyrrole, green reduced graphene oxide (gRGO) and cholesterol oxidase (ChOx). The graphene oxide (GO) was “green” reduced, with the Paederia foetide (PFL) plant extract at 60 °C to improve its biocompatibility. The adherence of gRGO to the electrode surface was improved through its co‐deposition during electro‐polymerization of pyrrole. Interestingly, the enzyme ChOx also could be immobilized during this co‐deposition process, resulting in one step biosensor fabrication. The optimization of electrochemical deposition parameters on glassy carbon electrode for this enzymatic bioelectrode (ChOx‐gRGO‐PPy/GCE) has resulted in excellent sensitivity (1095.3 μA mM−1 cm−2), wide linear range (0.01–6 mM), and low detection limit (3.78 μM) of cholesterol. Also, the Dubinin‐Radushkevitch (D−R) adsorption study revealed the occurrence of physisorption (ϵ=3.35) process in this bioelectrode, confirming diffusion‐controlled reaction. Reproducibility of electrodeposition procedure, impeccable selectivity in the presence of interferrants, repeatability for multiple use and long‐term stability for practical use were also ensured. This could be used to construct the cost‐effective device for cholesterol diagnosis.

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“…Graphite rods of 6 mm diameter and of 0.28 cm 2 geometric area were electrochemically modified by bioselective layer using single‐step based procedure . Prior to the modification, the surfaces of the graphite rods were mechanically polished until the ‘mirror finish surface’ was observed.…”

Section: Methodsmentioning

confidence: 99%

“…Deposition of composite layer based on polypyrrole, glucose oxidase and Prussian Blue (Ppy/GOx/PB) can be performed in Fe 2+ , [Fe(CN) 6 ] 4− , pyrrole and glucose oxidase containing solution by single‐step electrochemical procedure , during which PB and GOx are entrapped within formed conducting polymer‐Ppy. Moreover, both entrapped compounds (GOx and PB) appears in very close proximity, therefore, significantly higher surface area of PB becomes involved into catalytic action.…”

Section: Introductionmentioning

confidence: 99%

“…The deposition of Ppy/GOx/PB using the mixture of Fe 2+ , [Fe(CN) 6 ] 4− ions seems to be one of the best ways to form glucose biosensor, because Prussian blue and GOx are being assemble in bio‐active layer simultaneously without additional acidification of deposition solution. Synthesis of PB from traditionally used aqueous solutions containing a mixture of ferric (Fe 3+ ) and ferricyanide ([Fe 3+ (CN) 6 ] 3− ) ions may cause inactivation of enzyme, because FeCl 3 is stabile only at relatively low pH, which is harmful for GOx, while acidic pH is not necessary for Prussian blue formation from Fe 2+ salts mixture .…”

Section: Introductionmentioning

confidence: 99%

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Physicochemical Characteristics of Polypyrrole/(Glucose oxidase)/(Prussian Blue)‐based Biosensor Modified with Ni‐ and Co‐Hexacyanoferrates

et al. 2018

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In this work, three types of electrodes suitable for amperometric glucose biosensors were designed. One type of electrode was based on bio‐selective layer of polypyrrole/(glucose oxidase)/(Prussian Blue) (Ppy/GOx/PB) and it was used as a control electrode regarding to which electrochemical properties of two other types of electrodes were compared. During the formation of Prussian blue layers graphite electrodes were additionally modified by Ni‐hexacyanoferrate (NiHCF) and by Co‐hexacyanoferrate (CoHCF) in order to design Ppy/GOx/PB‐NiHCF and Ppy/GOx/PB‐CoHCF electrodes, respectively. Some physicochemical characteristics of all three types of electrodes were evaluated and compared. The Ppy/GOx/PB‐NiHCF electrode showed wider linear range of the calibration curve than Ppy/GOx/PB and Ppy/GOx/PB‐CoHCF electrodes. The effect of temperature on analytical performance of the Ppy/GOx/PB‐NiHCF based biosensor has been evaluated and activation energy of enzyme catalysed reaction has been calculated within the temperature range of 15 °C to 30 °C.

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Single-step procedure for the modification of graphite electrode by composite layer based on polypyrrole, Prussian blue and glucose oxidase

Cited by 58 publications

References 19 publications

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

One Step Electrode Fabrication for Direct Electron Transfer Cholesterol Biosensor Based on Composite of Polypyrrole, Green Reduced Graphene Oxide and Cholesterol Oxidase

Physicochemical Characteristics of Polypyrrole/(Glucose oxidase)/(Prussian Blue)‐based Biosensor Modified with Ni‐ and Co‐Hexacyanoferrates

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