Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery

Li, Yujing; Lee, Robert J.; Huang, Xueqin; Li, Yuhuan; Lv, Bingcong; Wang, Tianpeng; Qi, Yuhang; Fei, Hao; Lü, Jiahui; Meng, Qingfan; Teng, Lirong; Zhou, Yanmin; Xie, Jingjing; Teng, Lesheng

doi:10.1016/j.nano.2016.09.014

Cited by 45 publications

(30 citation statements)

References 24 publications

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“…According to previous research, the inverted W-type microfluidic chip was designed independently[9] , MF-NPs-CTX were prepared by the microfluidic chip. Specifically, a 20.0 mg/mL CTX ethanol solution and a 12.5% sodium chloride solution were prepared and incubated in a 40 °C water bath with HSA (200 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

“…NPs have been prepared by emulsification, desolvation, or nab-technology, but disadvantages included toxic reagent residues, complicated preparation processes, and poor repeatability between batches. Recently, excellent progress has been made in preparing NPs based on MF technology [8,9]. MF enables precise manipulation of liquid flow in the micron size range, allowing the organic and aqueous phases to be rapidly mixed to produce NPs with uniform particle size distribution, high drug encapsulation efficiency (EE), and small batch-to-batch variation.…”

Section: Introductionmentioning

confidence: 99%

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Folate Receptor-Targeted Albumin Nanoparticles Based on Microfluidic Technology to Deliver Cabazitaxel

Meng

Sun

Lee

et al. 2019

Cancers

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Microfluidic technology (MF) has improved the formulation of nanoparticles (NPs) by achieving uniform particle size distribution, controllable particle size, and consistency. Moreover, because liquid mixing can be precisely controlled in the pores of the microfluidic chip, maintaining high mixing efficiency, MF exerts higher of NP encapsulation efficiency (EE) than conventional methods. MF-NPs-cabazitaxel (CTX) particles (MF-NPs-CTX) were first prepared by encapsulating CTX according to MF. Folate (FA)- Polyethylene glycol (PEG)-NPs-CTX particles (FA-PEG-NPs-CTX) were formulated by connecting FA to MF-NPs-CTX to endow NPs with targeted delivery capability. Accordingly, the mean particle size of FA-PEG-NPs-CTX increased by approximately 25 nm, as compared with MF-NPs-CTX. Upon morphological observation of FA-PEG-NPs-CTX and MF-NPs-CTX by transmission electron microscopy (TEM), all NPs were spherical and particle size distribution was uniform. Moreover, the increased delivery efficiency of CTX in vitro and its strong tumor inhibition in vivo indicated that FA-PEG-NPs-CTX had a powerful tumor-suppressive effect both in vitro and in vivo. In vivo imaging and pharmacokinetic data confirmed that FA-PEG-NPs-CTX had good drug delivery efficiency. Taken together, FA-PEG-NPs-CTX particles prepared using MF showed high efficient and targeted drug delivery and may have a considerable driving effect on the clinical application of targeting albumin NPs.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Folate Receptor-Targeted Albumin Nanoparticles Based on Microfluidic Technology to Deliver Cabazitaxel

Meng

Sun

Lee

et al. 2019

Cancers

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“…Therefore, passive mixing can be achieved at a wide range of flow rates in a Tesla mixer. Herringbone micromixers enable the controlled and rapid mixing of two input streams by groove‐induced microvortices . A three‐inlet microfluidic device (Figure F) has also been widely applied to generate tunable dual microvortices for creating well‐controlled liquid–liquid mixing required for homogeneous self‐assembly of amphiphiles .…”

Section: Microfluidics For Fabrication Of Sddss With Well‐controlled mentioning

confidence: 99%

Microfluidics for Cancer Nanomedicine: From Fabrication to Evaluation

Zhang

Zhu

Shen

2018

Small

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Self-assembled drug delivery systems (sDDSs), made from nanocarriers and drugs, are one of the major types of nanomedicines, many of which are in clinical use, under preclinical investigation, or in clinical trials. One of the hurdles of this type of nanomedicine in real applications is the inherent complexity of their fabrication processes, which generally lack precise control over the sDDS structures and the batch-to-batch reproducibility. Furthermore, the classic 2D in vitro cell model, monolayer cell culture, has been used to evaluate sDDSs. However, 2D cell culture cannot adequately replicate in vivo tissue-level structures and their highly complex dynamic 3D environments, nor can it simulate their functions. Thus, evaluations using 2D cell culture often cannot correctly correlate with sDDS behaviors and effects in humans. Microfluidic technology offers novel solutions to overcome these problems and facilitates studying the structure-performance relationships for sDDS developments. In this Review, recent advances in microfluidics for 1) fabrication of sDDSs with well-defined physicochemical properties, such as size, shape, rigidity, and drug-loading efficiency, and 2) fabrication of 3D-cell cultures as "tissue/organ-on-a-chip" platforms for evaluations of sDDS biological performance are in focus.

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“…With the miniaturization of biological and medical systems, biomaterials with small sizes and monodispersion have attracted great attention . Nanohybrids composed of polymer and organic molecules are one of the most important biomaterials.…”

Section: Microfluidic Synthesis Of Nanohybridsmentioning

confidence: 99%

Microfluidic Synthesis of Nanohybrids

Wang

Song

2017

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Nanohybrids composed of two or more components exhibit many distinct physicochemical properties and hold great promise for applications in optics, electronics, magnetics, new energy, environment protection, and biomedical engineering. Microfluidic systems exhibit many advantages due to their unique characteristics of narrow channels, variable length, controllable number of channels and multiple integrations. Particularly their spatial-temporarily splitting of the formation stages during nanomaterials formation along the microfluidic channels favors the online control of the reaction kinetic parameters and in situ tuning of the product properties. This Review is focused on the features of the current types of microfluidic devices in the synthesis of different types of nanohybrids based on the classification of the four main kinds of materials: metal, nonmetal inorganic, polymer and composites. Their morphologies, compositions and properties can be adjusted conveniently in these synthesis systems. Synthesis advantages of varieties of microfluidic devices for specific nanohybrids of defined surfaces and interfaces are presented according to their process and microstructure features of devices as compared with conventional methods. A summary is presented, and challenges are put forward for the future development of the microfluidic synthesis of nanohybrids for advanced applications.

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Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery

Cited by 45 publications

References 24 publications

Folate Receptor-Targeted Albumin Nanoparticles Based on Microfluidic Technology to Deliver Cabazitaxel

Folate Receptor-Targeted Albumin Nanoparticles Based on Microfluidic Technology to Deliver Cabazitaxel

Microfluidics for Cancer Nanomedicine: From Fabrication to Evaluation

Microfluidic Synthesis of Nanohybrids

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