Single-step label-free nanowell immunoassay accurately quantifies serum stress hormones within minutes

Mahmoodi, Seyed Reza; Xie, Pengfei; Zachs, Daniel P.; Peterson, Erik; Graham, Rachel S.; Kaiser, C.; Lim, Hubert H.; Allen, Mark G.; Javanmard, Mehdi

doi:10.1126/sciadv.abf4401

Cited by 22 publications

(23 citation statements)

References 56 publications

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“…For instance, this phenomenon can be used to accelerate the immobilization of recognizing elements on electrodes by applying AC signals to construct biosensing architectures . This step can be as short as 15 min in this scenery . Indeed, although carbon typically affords a moderate antifouling ability, SiO 2 NPs were revealed to passivate graphite electrodes as well (Figure S10).…”

Section: Resultsmentioning

confidence: 99%

“…85 This step can be as short as 15 min in this scenery. 86 Indeed, although carbon typically affords a moderate antifouling ability, 87 SiO 2 NPs were revealed to passivate graphite electrodes as well (Figure S10). We attempted to prevent this fouling issue through the coating of Au IDEs with the dielectric film (50 nm SiO 2 by sputtering).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Real-Time and In Situ Monitoring of the Synthesis of Silica Nanoparticles

et al. 2022

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The real-time and in situ monitoring of the synthesis of nanomaterials (NMs) remains a challenging task, which is of pivotal importance by assisting fundamental studies (e.g., synthesis kinetics and colloidal phenomena) and providing optimized quality control. In fact, the lack of reproducibility in the synthesis of NMs is a bottleneck against the translation of nanotechnologies into the market toward daily practice. Here, we address an impedimetric millifluidic sensor with data processing by machine learning (ML) as a sensing platform to monitor silica nanoparticles (SiO2NPs) over a 24 h synthesis from a single measurement. The SiO2NPs were selected as a model NM because of their extensive applications. Impressively, simple ML-fitted descriptors were capable of overcoming interferences derived from SiO2NP adsorption over the signals of polarizable Au interdigitate electrodes to assure the determination of the size and concentration of nanoparticles over synthesis while meeting the trade-off between accuracy and speed/simplicity of computation. The root-mean-square errors were calculated as ∼2.0 nm (size) and 2.6 × 1010 nanoparticles mL–1 (concentration). Further, the robustness of the ML size descriptor was successfully challenged in data obtained along independent syntheses using different devices, with the global average accuracy being 103.7 ± 1.9%. Our work advances the developments required to transform a closed flow system basically encompassing the reactional flask and an impedimetric sensor into a scalable and user-friendly platform to assess the in situ synthesis of SiO2NPs. Since the sensor presents a universal response principle, the method is expected to enable the monitoring of other NMs. Such a platform may help to pave the way for translating “sense–act” systems into practice use in nanotechnology.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Real-Time and In Situ Monitoring of the Synthesis of Silica Nanoparticles

et al. 2022

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“…However, these labeling methods cause false-positive/negative occurrences in biological assays due to alterations in physicochemical or intermolecular binding properties [ 22 , 23 ]. To solve this problem, label-free methods for biomolecular detection have been developed, such as electrochemical [ 24 , 25 , 26 ], surface plasmon resonance [ 27 , 28 ], and nanowell assay [ 29 , 30 , 31 ]. The label-free immunoassay methods are attracting attention because of their advantages, such as easy fabrication [ 32 ] and low cost [ 33 ], while excluding the effect of the labels [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Hemolysis-Inspired, Highly Sensitive, Label-Free IgM Detection Using Erythrocyte Membrane-Functionalized Nanomechanical Resonators

et al. 2022

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Immunoglobulin detection is important for immunoassays, such as diagnosing infectious diseases, evaluating immune status, and determining neutralizing antibody concentrations. However, since most immunoassays rely on labeling methods, there are limitations on determining the limit of detection (LOD) of biosensors. In addition, although the antigen must be immobilized via complex chemical treatment, it is difficult to precisely control the immobilization concentration. This reduces the reproducibility of the biosensor. In this study, we propose a label-free method for antibody detection using microcantilever-based nanomechanical resonators functionalized with erythrocyte membrane (EM). This label-free method focuses on the phenomenon of antibody binding to oligosaccharides (blood type antigen) on the surface of the erythrocyte. We established a method for extracting the EM from erythrocytes and fabricated an EM-functionalized microcantilever (MC), termed EMMC, by surface-coating EM layers on the MC. When the EMMC was treated with immunoglobulin M (IgM), the bioassay was successfully performed in the linear range from 2.2 pM to 22 nM, and the LOD was 2.0 pM. The EMMC also exhibited excellent selectivity compared to other biomolecules such as serum albumin, γ-globulin, and IgM with different paratopes. These results demonstrate that EMMC-based nanotechnology may be utilized in criminal investigations to identify blood types with minimal amounts of blood or to evaluate individual immunity through virus-neutralizing antibody detection.

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“…(17) Furthermore, some biosensors are also label-free, enabling the direct detection of target substances. (18,19) In this study, we developed a label-free immunosensor with the aim of achieving rapid and straightforward 11-KT measurement as a foundation for future point of care of fish (Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Development of Label-free Immunosensor System toward Gender Determination of Fish

Wu¹,

Sakurai²,

Saito³

et al. 2021

Sensors and Materials

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It is imperative to discriminate between male and female fish during the juvenile stage and pre-adjust the required numbers of males and females to efficiently raise parent fish in aquaculture farms. One method to discriminate between males and females is to measure a fishspecific steroid hormone such as 11-ketotestosterone (11-KT) in fish plasma. However, conventional methods such as enzyme-linked immunosorbent assay (ELISA) are complicated to operate and require a long time for analysis. Here, we introduce a novel immunosensor system that combines immunological reaction and electrochemical measurement with the aim of achieving a rapid and straightforward measurement method in preparation for gender determination of fish. Using our system, we observed stepwise decreases in the oxidation peak current value with increasing concentration of 11-KT in the sample. Furthermore, a strong linear relationship was obtained between the standard solution concentrations of 0.21 and 3.30 pmol ml −1 (R = 0.9869) in only 20 min. Next, the optimum operating conditions of the sensor were evaluated. It was also confirmed that the sensor did not respond to other hormones. Moreover, a strong linear relationship was obtained between the values and those obtained by the conventional ELISA method (R = 0.9927).

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Single-step label-free nanowell immunoassay accurately quantifies serum stress hormones within minutes

Cited by 22 publications

References 56 publications

Real-Time and In Situ Monitoring of the Synthesis of Silica Nanoparticles

Real-Time and In Situ Monitoring of the Synthesis of Silica Nanoparticles

Hemolysis-Inspired, Highly Sensitive, Label-Free IgM Detection Using Erythrocyte Membrane-Functionalized Nanomechanical Resonators

Development of Label-free Immunosensor System toward Gender Determination of Fish

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