Single-step label-free hepatitis B virus detection by a piezoelectric biosensor

Giamblanco, Nicoletta; Conoci, Sabrina; Russo, Dario; Marletta, Giovanni

doi:10.1039/c5ra03467a

Cited by 40 publications

(35 citation statements)

References 47 publications

(36 reference statements)

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“…Conventional diagnostic techniques such as microscopy, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR) are well established gold standards for the diagnosis of many infectious diseases. However, these assays involve tedious procedures, skilled operators, and expensive instrumentation, all of which translate into high assay costs and significant delays between sample collection and medical diagnosis ( Giamblanco et al, 2015 ; Ragavan et al, 2018 ). These shortcomings become especially prominent in resource-limited settings, where many infections are undiagnosed due to the poor access to diagnostic services ( Sharma et al, 2015 ; Sin et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Lim

Saha

Tey

et al. 2020

Biosensors and Bioelectronics

147

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Infectious diseases are the ever-present threats to public health and the global economy. Accurate and timely diagnosis is crucial to impede the progression of a disease and break the chain of transmission. Conventional diagnostic techniques are typically time-consuming and costly, making them inefficient for early diagnosis of infections and inconvenient for use at the point of care. Developments of sensitive, rapid, and affordable diagnostic methods are necessary to improve the clinical management of infectious diseases. Quartz crystal microbalance (QCM) systems have emerged as a robust biosensing platform due to their label-free mechanism, which allows the detection and quantification of a wide range of biomolecules. The high sensitivity and short detection time offered by QCM-based biosensors are attractive for the early detection of infections and the routine monitoring of disease progression. Herein, the strategies employed in QCM-based biosensors for the detection of infectious diseases are extensively reviewed, with a focus on prevalent diseases for which improved diagnostic techniques are in high demand. The challenges to the clinical application of QCM-based biosensors are highlighted, along with an outline of the future scope of research in QCM-based diagnostics.

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Section: Introductionmentioning

confidence: 99%

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Lim

Saha

Tey

et al. 2020

Biosensors and Bioelectronics

147

View full text Add to dashboard Cite

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“…The fabrication processes can be summarised in two steps: (a) in situ synthesis of the capture probe and (b) the microspotting of pre-synthesized capture probes by contact or noncontact printing technologies [ 16 ]. In this context, the probe immobilization strategy plays a key role, strongly influencing probe density and, then, hybridization processes [ 17 , 18 , 19 ]. Many chemistry strategies suitable for covalent capture probe attachment are reported in the literature, such as epoxy-amine, aldehyde-amine, metal-sulphur, biotin-streptavidin, and amine- N -hydroxy succinimide chemistries [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

An Advanced, Silicon-Based Substrate for Sensitive Nucleic Acids Detection

Petralia

Vicario

Calabrese

et al. 2018

Sensors

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Surface substrate and chemical functionalization are crucial aspects for the fabrication of the sensitive biosensor based on microarray technology. In this paper, an advanced, silicon-based substrate (A-MA) allowing enhancement of optical signal for microarray application is described. The substrate consists in a multilayer of Si/Al/SiO2 layers. The optical signal enhancement is reached by a combination of the mirror effect of Al film and the SiO2 thickness around 830 nm, which is able to reach the maximum of interference for the emission wavelength of the Cy5 fluorescent label. Moreover, SiO2 layer is suitable for the immobilization of single-strand DNA through standard silane chemistry, and probe densities of about 2000 F/µm2 are reached. The microarray is investigated in the detection of HBV (Hepatitis B Virus) pathogen with analytical samples, resulting in a dynamic linear range of 0.05–0.5 nM, a sensitivity of about 18000 a.u. nM−1, and a Limit of Detection in the range of 0.031–0.043 Nm as a function of the capture probe sequence.

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“…The most recent example in this case is the development of a single-step, label-free QCM-based HepBV biosensor by Giamblanco et al [105]. The HepBV genome consists of a double stranded DNA.…”

Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

“…Hereby, some of the most promising results of DNA-QCM biosensors for different viruses are discussed. For instance, DNA-QCM biosensors have been successfully tested for the detection of hepatitis B virus (HepBV) [105][106][107], hepatitis C virus (HepCV) [117], vaccinia virus [108], viral haemorrhagic septicaemia virus (VHSV) [109], human papilloma virus (HPV) [110], dengue virus [118], and orchid viruses such as cymbidium mosaic virus (CymMV) and odontoglossum ringspot virus (ORSV) [111].…”

Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

“…The HepBV genome consists of a double stranded DNA. Giamblanco et al [105] fabricated a device for detecting HepBV DNA by immobilizing thiolated single stranded DNA (ssDNA) oligonucleotide probes on the surface of QCM gold electrode. The detection of HepBV genome was achieved via hybridization between ssDNA probe and viral DNA.…”

Section: Dna and Aptamers For Qcm Virus Sensorsmentioning

confidence: 99%

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Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Single-step label-free hepatitis B virus detection by a piezoelectric biosensor

Abstract: Probe densityvs.genome recognition selectivity.

Cited by 40 publications

References 47 publications

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

Quartz crystal microbalance-based biosensors as rapid diagnostic devices for infectious diseases

An Advanced, Silicon-Based Substrate for Sensitive Nucleic Acids Detection

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

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