Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay

Egerer, Karl; Roggenbuck, Dirk; Büttner, Thomas; Lehmann, B.; Kohn, Annushka; Landenberg, Philipp von; Hiemann, Rico; Feist, Eugen; Burmester, Gerd‐Rüdiger; Dörner, Thomas

doi:10.1186/ar3421

Cited by 38 publications

(42 citation statements)

References 35 publications

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“…Western blot is the other technique used recently in aPL determination (Egerer et al, 2011). It was published that it gives similar results as ELISA, although the results are only qualitative.…”

Section: New Trends In Apl Determinationmentioning

confidence: 73%

Antiphospholipid Antibodies – Detection and Clinical Importance

Swadźba¹,

Kołodziejczyk²

2012

Antiphospholipid Syndrome

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Section: New Trends In Apl Determinationmentioning

confidence: 73%

Antiphospholipid Antibodies – Detection and Clinical Importance

Swadźba¹,

Kołodziejczyk²

2012

Antiphospholipid Syndrome

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“…Данные методы твердофазного анализа характеризуются новыми подходами к сорбции антигена, обеспечивая большую плотность антигена на твердофазном носителе. Особенностью ЛБ является высокое сродство фосфолипидов к PVDF-мембране, что позволяет ориентировать гидрофильные участки молекул, обеспечивая их взаимодействие с белковыми кофакторами [11]. Результатом оптимизации характеристик твердой фазы становится увеличение аналитической чувствительности теста и расширение диапазона измеряемых показателей.…”

Section: Discussionunclassified

“…Особого внимания заслуживает метод лайнблоттинга (ЛБ) [11]. Преимуществом ЛБ при сравнении с ИФА является «мультиплексный» подход, позволяющий единовременно детекти-ровать до 10 разновидностей АФА.…”

Section: Introductionunclassified

Ranging of Antiphospolipid Antibodies in the Patients With Thrombophilia and Recurrent Miscarriage

et al. 2018

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Laboratory diagnosis of antiphospholipid syndrome (APS) is based on detection of antiphospholipid antibodies (aPLs). E.g., aPLs are directed against conformational epitopes of the so-called “co-factor” proteins: β2-gycoprotein 1 (β2-GP1), annexin V (An V) and prothrombin (Pt) that are formed during interaction with phospholipids – cardiolipin (CL), phosphatic acid (Pha), phosphatidylcholine (Pch), phosphatidylethanolamine (Pe), phosphatidylglycerol (Pg), phosphatidylinositol (Pi), phosphatidylserine (Ps). A routine methodology of detection based on ELISA testing is challenged by new tests when the antigen is absorbed on another kind of support like microbeads or membranes that can influence density of conformational epitopes for aPL’s binding. The aim of our study was to compare the results of aPLs detection by ELISA and multi-line immunodot assay (MLD). We collected blood serum samples from 45 patients with noncardioembolic ischemic strokes, 19 patients with recurrent deep vein thrombosis of lower limbs, 44 females with recurrent miscarriages, and 50 clinically healthy donors. To compare the results of aPL detection by ELISA and MLD kits, the test systems from different manufacturers were evaluated. We used an ELISA kits for detection of antibodies to CL IgG, aCL IgM, β2-GP1 produced by Euroimmun AG (Mr1) and Orgentec Diagnostica GmbH (Mr2) and MLD – for detection of antibodies to CL, β2-GP1, Pch, Pe, Pg, Pi, Ps, AnV and Pt (Medipan GmbH, Mr3). When a cut-off titer was used as the main index, 30.5% of patients were aPLs-positive with ELISA method by Mr1 and 38%, wiht Mr2. By MLD aPls were detected in 30% of patients. In the same cohort, medium and high aPLs titers (> 40 U/mL) were determined in 12% of patients using ELISA kits. Positive and highly positive aPLs titers were determined in 16% when using a new method by Mr3. Medium and high titer were detected only for antibodies to β2-GP1, CL, An V, Pha and Phs. The use of ELISA approach for detection of aPLs in patients with thrombosis and obstetric pathology is associated with relatively high number of low-positive ELISA results. Due to higher sensitivity for medium and high aPLs titers, MLD testing may be used as a confirming method for APS diagnosis.

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“…The most common epitopes against which aPL antibodies react are phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl-DL-glycerol, phosphatidylinositol, phosphatidyl-L-serine and annexin V [19]. Sato et al .…”

Section: Discussionmentioning

confidence: 99%

Antiphospholipid antibodies in localized scleroderma: the potential role of screening tests for the detection of antiphospholipid syndrome

Lis‐Święty¹,

Brzeźińska-Wcisło²,

Arasiewicz³

et al. 2014

pdia

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IntroductionThe presence of antiphospholipid antibodies (aPL) is associated with infections, drugs and autoimmune disorders. Those antibodies are also detected in approximately 5–20% of the healthy population. The presence of aPL can lead to the occurrence of thrombotic events or abortion, which define the antiphospholipid syndrome (APS).AimTo evaluate the potential role of aPL in diagnosing APS in patients with localized scleroderma (LoS).Material and methodsSerum samples from 45 patients with various forms of LoS were examined. They were screened with the commercially-available immunodot assay Anti-Phospholipid 10 Dot (GA Generic Assays GmbH, Dahlewitz, Germany). A number of clinical and laboratory parameters, especially APS symptoms, were assessed in patients with positive aPL: arterial and venous thrombotic events, obstetric complications, thrombocytopenia and neurological symptoms.ResultsThe following profile of aPL IgG or IgM was obtained from patients with LoS: cardiolipin 15/45, phosphatidic acid 41/45, phosphatidyl-choline 0/45, -ethanolamine 6/45, -glycerole 1/45 (patient with Lyme disease), -inositol 7/45, -serine 14/45, annexin V 34/45, β2GPI 21/45, prothrombin 30/45. Antiphospholipid antibodies profile screening in these individuals revealed two cases of suspected secondary laboratory APS. However, no such clinical and laboratory parameters were found in other LoS patients with positive aPL. Similarly, no association was found between the presence of aPL and either thrombotic events or other APS symptoms.ConclusionsAntiphospholipid antibodies are commonly found in patients with LoS but the exact role of these antibodies remains unclear. Clinical manifestations of APS are not frequently seen during LoS.

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Single-step autoantibody profiling in antiphospholipid syndrome using a multi-line dot assay

Cited by 38 publications

References 35 publications

Antiphospholipid Antibodies – Detection and Clinical Importance

Antiphospholipid Antibodies – Detection and Clinical Importance

Ranging of Antiphospolipid Antibodies in the Patients With Thrombophilia and Recurrent Miscarriage

Antiphospholipid antibodies in localized scleroderma: the potential role of screening tests for the detection of antiphospholipid syndrome

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