Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel composite

Park, Y.B.; Ha, Chul‐Won; Kim, J.A.; Han, Won Suk; Rhim, Jong Kook; Lee, H.J.; Kim, K.J.; Park, Y.G.; Chung, Jae Youn

doi:10.1016/j.joca.2016.10.012

Cited by 70 publications

(43 citation statements)

References 44 publications

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“…The process of stem cell isolation and cultivation is easier in comparison to primary chondrocytes or osteoblasts [38,39]. The osteogenic and chondrogenic differentiation potential of MSCs in conjunction with the engrafting ability are crucial for cartilage [17,40,41] and bone regeneration in vivo [42]. Current literature describes examples of the beneficial effects of blood product supplementation on adi-pogenic [43], chondrogenic [44][45][46], and osteogenic differentiation of MSCs [45,47].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Locations

Neubauer

Kuten

Stotter

et al. 2019

Stem Cells International

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Background. Adipose-derived mesenchymal stem cells (AD-MSCs) from fat tissue considered “surgical waste” during joint surgery may provide a potent source for regenerative medicine. Intra-articular, homologous fat tissue (Hoffa’s fat pad, pouch fat) might possess a superior chondrogenic and osteogenic differentiation potential in comparison to extra-articular, nonhomologous fat. Blood products might further enhance this potential. Methods. AD-MSCs were isolated from fat tissue of 3 donors from 3 locations each, during total knee replacement. Isolated cells were analyzed via flow cytometry. Cells were supplemented with blood products: two types of platelet-rich plasma (EPRP—PRP prepared in the presence of EDTA; CPRP—PRP prepared in the presence of citrate), hyperacute serum (hypACT), and standard fetal calf serum (FCS) as a positive control. The viability of the cells was determined by XTT assay, and the progress of differentiation was tested via histological staining and monitoring of specific gene expression. Results. Blood products enhance ex vivo cell metabolism. Chondrogenesis is enhanced by EDTA-PRP and osteogenesis by citrate PRP, whereas hyperacute serum enhances both differentiations comparably. This finding was consistent in histological analysis as well as in gene expression. Lower blood product concentrations and shorter differentiation periods lead to superior histological results for chondrogenesis. Both PRP types had a different biological effect depending upon concentration, whereas hyperacute serum seemed to have a more consistent effect, independent of the used concentration. Conclusion. (i) Blood product preparation method, (ii) type of anticoagulant, (iii) differentiation time, and (iv) blood product concentration have a significant influence on stem cell viability and the differentiation potential, favouring no use of anticoagulation, shorter differentiation time, and lower blood product concentrations. Cell-free blood products like hyperacute serum may be considered as an alternative supplementation in regenerative medicine, especially for stem cell therapies.

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Section: Discussionmentioning

confidence: 99%

“…GAPDH was used as a housekeeping gene. PCR conditions were optimized for annealing temperature and limited cycle number (40) to ensure that product formation was in the responsive range.…”

Section: Metabolic Activity Assay (Xtt Assay)mentioning

confidence: 99%

The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Locations

Neubauer

Kuten

Stotter

et al. 2019

Stem Cells International

View full text Add to dashboard Cite

show abstract

“…28 Satué et al 28 demonstrated the safety and efficacy of IA-injected MSCs in the knee and stated that MSCs remained in the synovial cavity, engrafted within the cartilage lesion, and were detectable up to 1 month postinjection; no adverse effects after injection were observed. 28 Other authors also confirmed the presence of MSCs in the knee joint at 3 months, 29 8 weeks, 30 and 14 days after implantation. 31 Numerous studies have reported that the intravenous infusion or intra-articular transplantation of various types of MSCs improves arthritic symptoms in animal models by paracrine mechanisms.…”

Section: Introductionmentioning

confidence: 71%

Systematic Review of Human Dental Pulp Stem Cells for Cartilage Regeneration

Fernandes

SantAnna

Frisene

et al. 2020

Tissue Engineering Part B: Reviews

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Background: Symptomatic cartilage lesions and early osteoarthritis produce significant clinical and economic burdens. Cartilage repair can improve the symptoms and delay arthroplasty. The complete healing of damaged cartilage with the consistent reproduction of normal hyaline cartilage has not yet been achieved. The choice of harvesting site might influence the cells' abilities to modulate immunologic and inflammatory responses. Recently, dental pulp has been shown to contain a stem cell niche consisting of dental pulp stem cells (DPSCs) that maintain their self-renewal capacity due to the active environment in the dental pulp of deciduous teeth. Objective: The aim of this study was to critically review the current literature on the potential and limitations of the use of dental pulp-derived mesenchymal stem cells in cell-based therapies for cartilage regeneration. Methods: An electronic, customized search of scientific articles was conducted using the PubMed/MEDLINE and EMBASE databases from their inception to December 2018. The inclusion criteria were applied, and the articles that described the use of DPSC in cartilage treatment were selected for complete evaluation. The articles were classified according to the scaffold used, experimental model, chondrogenic differentiation features, defect location, cartilage evaluation, and results. After the application of the eligibility criteria, a total of nine studies were selected and fully analyzed. Results: A variety of animal models were used, including mice, rats, rabbits, and miniature pigs, to evaluate the quality and safety of human DPSCs in the repair of cartilage defects. Among the articles, two studies focused on preclinical models of cartilage tissue engineering. Five studies implanted DPSCs in other animal sites. Conclusion: The use of DPSCs is a potential new stem cell therapy for articular cartilage repair. The preclinical evidence discussed in this article provides a solid foundation for future clinical trials.

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“…Previous cell tracking results after intra-articular cell transplantation show gradual decrease of transplanted cells over time 29,30 . A cartilage repair study utilizing human umbilical cord blood-derived MSC-hyaluronic acid gel composite in a rabbit model resulted in disappearance of human origin cells by the 16 th wk 31 . One of the strengths of this study is the demonstration of the TEC's continued presence and differentiation after in vivo www.nature.com/scientificreports www.nature.com/scientificreports/ transplantation, as shown in our remodeling and cell distribution data, Time-dependent cartilage differentiation ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair

Park

Min

Park

et al. 2020

Sci Rep

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The aim of this study was to develop a fetal cartilage-derived progenitor cell (FCPC) based cartilage gel through self-assembly for cartilage repair surgery, with clinically useful properties including adhesiveness, plasticity, and continued chondrogenic remodeling after transplantation. characterization of the gels according to in vitro self-assembly period resulted in increased chondrogenic features over time. Adhesion strength of the cartilage gels were significantly higher compared to alginate gel, with the 2-wk group showing a near 20-fold higher strength (1.8 ± 0.15 kPa vs. 0.09 ± 0.01 kPa, p < 0.001). The in vivo remodeling process analysis of the 2 wk cultured gels showed increased cartilage repair characteristics and stiffness over time, with higher integrationfailure stress compared to osteochondral autograft controls at 4 weeks (p < 0.01). In the nonhuman primate investigation, cartilage repair scores were significantly better in the gel group compared to defects alone after 24 weeks (p < 0.001). Cell distribution analysis at 24 weeks showed that human cells remained within the transplanted defects only. A self-assembled, FCPC-based cartilage gel showed chondrogenic repair potential as well as adhesive properties, beneficial for cartilage repair.

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Single-stage cell-based cartilage repair in a rabbit model: cell tracking and in vivo chondrogenesis of human umbilical cord blood-derived mesenchymal stem cells and hyaluronic acid hydrogel composite

Abstract: Transplanting hUCB-MSCs and HA composite promote cartilage repair and interactions between hUCB-MSCs and host cells initiated by paracrine action may play an important role in cartilage repair.

Cited by 70 publications

References 44 publications

The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Locations

The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Locations

Systematic Review of Human Dental Pulp Stem Cells for Cartilage Regeneration

Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair

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