Single-scan 2D NMR spectroscopy on a 25 T bitter magnet

Shapira, Boaz; Shetty, Kiran Kumar; Brey, Wallace S.; Gan, Zhehong; Frydman, Lucio

doi:10.1016/j.cplett.2007.06.016

Cited by 23 publications

(14 citation statements)

References 23 publications

(26 reference statements)

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“…The Keck magnet achieved a homogeneity of 48 ppm which was corrected to 12 ppm with the addition of ferromagnetic shims. The utility of a resistive magnet such as the Keck for wide line NMR was demonstrated [15] and pulse techniques to take advantage of the high field of this resistive magnet for high resolution NMR have been explored [12, 16]. The limitations of both stability and homogeneity, however, led to the Keck magnet serving as a test facility for further improvements in field homogeneity and suppression of field fluctuations [21] rather than as a tool for routine high-field NMR investigations.…”

Section: Sch Magnetmentioning

confidence: 99%

NMR spectroscopy up to 35.2 T using a series-connected hybrid magnet

Gan

Hung

Wang

et al. 2017

Journal of Magnetic Resonance

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136

195

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The National High Magnetic Field Laboratory has brought to field a Series-Connected Hybrid magnet for NMR spectroscopy. As a DC powered magnet it can be operated at fields up to 36.1 T. The series connection between a superconducting outsert and a resistive insert dramatically minimizes the high frequency fluctuations of the magnetic field typically observed in purely resistive magnets. Current-density-grading among various resistive coils was used for improved field homogeneity. The 48 mm magnet bore and 42 mm outer diameter of the probes leaves limited space for conventional shims and consequently a combination of resistive and ferromagnetic shims are used. Field maps corrected for field instabilities were obtained and shimming achieved better than 1 ppm homogeneity over a cylindrical volume of 1 cm diameter and height. The magnetic field is regulated within 0.2 ppm using an external 7Li lock sample doped with paramagnetic MnCl2. The improved field homogeneity and field regulation using a modified AVANCE NEO console enables NMR spectroscopy at 1H frequencies of 1.0, 1.2 and 1.5 GHz. NMR at 1.5 GHz reflects a 50% increase in field strength above the highest superconducting magnets available presently. Three NMR probes have been constructed each equipped with an external lock rf coil for field regulation. Initial NMR results obtained from the SCH magnet using these probes illustrate the very exciting potential of ultra-high magnetic fields.

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Section: Sch Magnetmentioning

confidence: 99%

NMR spectroscopy up to 35.2 T using a series-connected hybrid magnet

Gan

Hung

Wang

et al. 2017

Journal of Magnetic Resonance

Self Cite

136

195

View full text Add to dashboard Cite

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“…There have been multiple developments that improve this aspect, including non-uniform sampling [154,365] and projection–reconstruction [366,367]. There are also hardware approaches, including multiple receivers enabling 2 or more 2D experiments to be acquired simultaneously [368,369], as well as one-shot 2/3D methods which acquire different “increments” at different slices of the sample [370–376]. However, the latter put greater demands on sensitivity [377].…”

Section: Discussionmentioning

confidence: 99%

Applications of NMR spectroscopy to systems biochemistry

Fan

Lane

2016

Progress in Nuclear Magnetic Resonance Spectroscopy

168

169

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The past decades of advancements in NMR have made it a very powerful tool for metabolic research. Despite its limitations in sensitivity relative to mass spectrometric techniques, NMR has a number of unparalleled advantages for metabolic studies, most notably the rigor and versatility in structure elucidation, isotope-filtered selection of molecules, and analysis of positional isotopomer distributions in complex mixtures afforded by multinuclear and multidimensional experiments. In addition, NMR has the capacity for spatially selective in vivo imaging and dynamical analysis of metabolism in tissues of living organisms. In conjunction with the use of stable isotope tracers, NMR is a method of choice for exploring the dynamics and compartmentation of metabolic pathways and networks, for which our current understanding is grossly insufficient. In this review, we describe how various direct and isotope-edited 1D and 2D NMR methods can be employed to profile metabolites and their isotopomer distributions by stable isotope-resolved metabolomic (SIRM) analysis. We also highlight the importance of sample preparation methods including rapid cryoquenching, efficient extraction, and chemoselective derivatization to facilitate robust and reproducible NMR-based metabolomic analysis. We further illustrate how NMR has been applied in vitro, ex vivo, or in vivo in various stable isotope tracer-based metabolic studies, to gain systematic and novel metabolic insights in different biological systems, including human subjects. The pathway and network knowledge generated from NMR- and MS-based tracing of isotopically enriched substrates will be invaluable for directing functional analysis of other ‘omics data to achieve understanding of regulation of biochemical systems, as demonstrated in a case study. Future developments in NMR technologies and reagents to enhance both detection sensitivity and resolution should further empower NMR in systems biochemical research.

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“…These powered magnets have been successfully used for low resolution studies [5]. A number of explorative high-resolution NMR experiments have been demonstrated using 25 T and 33 T resistive [6][7][8][9], and 45 T hybrid [1,10] magnets including several experiments that are insensitive to magnetic field homogeneity and stability [11,12]. NMR experiments at even higher field up to 70 T have been performed using pulsed magnets [13,14].…”

Section: Introductionmentioning

confidence: 98%