Single-probe multistate detection of DNA via aggregation-induced emission on a graphene oxide platform

Tyagi, Abhishek; Chu, Kin Leung; Abidi, Irfan Haider; Cagang, Aldrine Abenoja; Zhang, Qicheng; Leung, Nelson; Zhao, Engui; Tang, Benzhong; Luo, Zhengtang

doi:10.1016/j.actbio.2016.12.003

Cited by 33 publications

(14 citation statements)

References 50 publications

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“…In a recent study [40], a graphene-based fluorescence biosensor was investigated for label-free detection of ssDNA and dsDNA based on aggregation-induced emission molecules (AIE) and complementary DNA (comDNA) adsorbed on GO. The sensing mechanisms rely on the capability of turning the AIE fluorescence on/off by adjusting its distance from the GO sheet.…”

Section: Graphene-based Biosensorsmentioning

confidence: 99%

Atomistic Simulations of Functionalized Nano-Materials for Biosensors Applications

Dutta

Corni

Brancolini

2022

IJMS

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Nanoscale biosensors, a highly promising technique in clinical analysis, can provide sensitive yet label-free detection of biomolecules. The spatial and chemical specificity of the surface coverage, the proper immobilization of the bioreceptor as well as the underlying interfacial phenomena are crucial elements for optimizing the performance of a biosensor. Due to experimental limitations at the microscopic level, integrated cross-disciplinary approaches that combine in silico design with experimental measurements have the potential to present a powerful new paradigm that tackles the issue of developing novel biosensors. In some cases, computational studies can be seen as alternative approaches to assess the microscopic working mechanisms of biosensors. Nonetheless, the complex architecture of a biosensor, associated with the collective contribution from “substrate–receptor–analyte” conjugate in a solvent, often requires extensive atomistic simulations and systems of prohibitive size which need to be addressed. In silico studies of functionalized surfaces also require ad hoc force field parameterization, as existing force fields for biomolecules are usually unable to correctly describe the biomolecule/surface interface. Thus, the computational studies in this field are limited to date. In this review, we aim to introduce fundamental principles that govern the absorption of biomolecules onto functionalized nanomaterials and to report state-of-the-art computational strategies to rationally design nanoscale biosensors. A detailed account of available in silico strategies used to drive and/or optimize the synthesis of functionalized nanomaterials for biosensing will be presented. The insights will not only stimulate the field to rationally design functionalized nanomaterials with improved biosensing performance but also foster research on the required functionalization to improve biomolecule–surface complex formation as a whole.

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Section: Graphene-based Biosensorsmentioning

confidence: 99%

Atomistic Simulations of Functionalized Nano-Materials for Biosensors Applications

Dutta

Corni

Brancolini

2022

IJMS

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“…Two dimensional (2D) nanomaterials with outstanding electronic and optical properties, such as broad absorbance, large surface area, and easily functionalized surface sites, can be utilized as acceptors or quenchers in FRET sensors [62,71,72]. To date, various nanomaterials, such as Au nanoparticles (NPs) [62], MoS 2 [73], graphene oxide (GO) [63,64], and MXenes [66], have been developed for the fluorescence detection of DNA for cancer diagnoses. Eftekhari-Sis et al developed a nanobiosensor based on graphene oxide (GO) and 5-carboxy fluorescein (FAM)-labeled DNA for the detection of a deletion mutation in exon 19 of the EGFR gene (Figure 2a) [65].…”

Section: Fret-based Biosensors For Detection Of Dna Targetsmentioning

confidence: 99%

“…This system can detect a target DNA in a small amount of sample solution in 10 min with a very low detection limit of 25 fmol/µL. Dhenadhayalan et al reported an ultrasensitive system based on molybdenum series (MoO 3 , MoS 2 , and MoSe 2 ) of 2D nanosheets (NSs) for the detection of a prostate-specific antigen (PSA) as a diagnostic biomarker of prostate cancer [63]. The detection was achieved with 13 pM of MoO 3 NSs, whereas MoS 2 and MoSe 2 NSs showed detection limits of 72 and 157 pM, respectively, among which the MoO 3 sensor system showed a fast fluorescence response within 2 min.…”

Section: Fret-based Biosensors For Detection Of Dna Targetsmentioning

confidence: 99%

Nanomaterial-Based Fluorescence Resonance Energy Transfer (FRET) and Metal-Enhanced Fluorescence (MEF) to Detect Nucleic Acid in Cancer Diagnosis

Choi

Shin

et al. 2021

Biomedicines

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Nucleic acids, including DNA and RNA, have received prodigious attention as potential biomarkers for precise and early diagnosis of cancers. However, due to their small quantity and instability in body fluids, precise and sensitive detection is highly important. Taking advantage of the ease-to-functionality and plasmonic effect of nanomaterials, fluorescence resonance energy transfer (FRET) and metal-enhanced fluorescence (MEF)-based biosensors have been developed for accurate and sensitive quantitation of cancer-related nucleic acids. This review summarizes the recent strategies and advances in recently developed nanomaterial-based FRET and MEF for biosensors for the detection of nucleic acids in cancer diagnosis. Challenges and opportunities in this field are also discussed. We anticipate that the FRET and MEF-based biosensors discussed in this review will provide valuable information for the sensitive detection of nucleic acids and early diagnosis of cancers.

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“…罗正汤等 [127] 利用 AIE 小分子和 GO 来检测不同的 DNA 序列. 将单链 DNA 引入化合物 58 的溶液中, 两者间通过静电相互作用紧密结合, 58 的分子内旋转受阻, 荧光强度明显增加; 当 GO 引入体系时, 单链 DNA 对 GO 有更强的结合力, 与 58 之间的作用力减弱, 由于不再有与 DNA 结合所成的刚性结构, 分子运动消耗能量, 导致荧光猝灭; 此外, 在 AIE-单链 DNA-GO 复合物中加入互补 DNA 后, 由于互补 DNA 与单链 DNA 杂交, 形成双螺旋 DNA 结构, 再次提供刚性环境, 能使荧光部分恢复.…”

Section: 生物传感unclassified

Recent Progress in Aggregation-Induced Emission-Active Organic Small Molecule Inorganic Nanocomposites

Gao¹,

Qin²,

Nie³

et al. 2020

Chin. J. Org. Chem.

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Fluorescent organic-inorganic nanocomposites have attracted more and more attention in the fields of chemical and biological sensing, biological imaging, energy materials, etc., due to their simple preparation, good biocompatibility and excellent imaging performance. Fluorescence quenching often occurs when traditional fluorescent organic small molecules are combined with inorganic materials, however, organic molecules with aggregation-induced emission (AIE) properties, which show high luminescence quantum yields in the aggregated state, provide opportunities for fluorescent organic-inorganic nanocomposites. Because of the unique advantages of the AIE fluorophore-functionalized inorganic nanomaterials, a great deal of research has been carried out on the design, synthesis and applications of such composite materials. The recent progress in the organic-inorganic composites of AIE-active organic small molecules and various types of inorganic nanomaterials (metal nanoparticles, perovskites, layered materials, oxides and sulfides, etc.) is summarized. In particular, the typical applications of these nanocomposites in chemical sensing, biosensing, bioimaging, drug transport, catalysis, photothermal therapy and energy materials are summarized. The prospects of these AIE-active organic-inorganic nanocomposites are also discussed.

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Single-probe multistate detection of DNA via aggregation-induced emission on a graphene oxide platform

Cited by 33 publications

References 50 publications

Atomistic Simulations of Functionalized Nano-Materials for Biosensors Applications

Atomistic Simulations of Functionalized Nano-Materials for Biosensors Applications

Nanomaterial-Based Fluorescence Resonance Energy Transfer (FRET) and Metal-Enhanced Fluorescence (MEF) to Detect Nucleic Acid in Cancer Diagnosis

Recent Progress in Aggregation-Induced Emission-Active Organic Small Molecule Inorganic Nanocomposites

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