Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines

Keeney, Michael; Chin‐Yee, Ian; Weir, Karin; Popma, Jan; Nayar, Rakash; Sutherland, D. Robert

doi:10.1002/(sici)1097-0320(19980415)34:2<61::aid-cyto1>3.0.co;2-f

“…cell population was used to identify the CD34? subpopulation according to the single-platform guidelines of the International Society of Hematotherapy and Graft Engineering (ISHAGE) [23]. Adding a known number of FlowCount fluorescent beads enabled the determination of an absolute CD34?…”

Section: Tnc Countmentioning

confidence: 99%

Quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation

Yamamoto

¹

,

Ikeda

²

,

Toyama

³

et al. 2011

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…In CD34ϩ HPC dual-platform analysis, the reference cells are the total leukocytes and no morphologically or phenotypically defined cell subset can be identified as the reference population. CD45 gating is also highly recommended when counting CD34ϩ HPC using the logical gate strategy developed by the International Society of Hematotherapy and Graft Engineering (ISHAGE; 119), and subsequently modified by others (47,51,53,120). The ISHAGE protocol and its modifications may be the best current examples of routinely applicable logical gating.…”

Section: Precise Identification Of Reference Populationsmentioning

confidence: 99%

“…FlowCount beads are larger than TruCount, and thus can be used with reduced FSC thresholding without a need for an associated fluorescent marker. However, an appropriate polygonal acquisition gate is necessary to eliminate the high amount of debris and electrical noise (53,120). The Beckman-Coulter method involves a fourcolor system using a single Argon-ion laser and the beads emit on all four channels (86,148).…”

Section: Count Standard and Spherotech-rainbow Beads But Theymentioning

confidence: 99%

Cytofluorometric methods for assessing absolute numbers of cell subsets in blood

Brando

¹

,

Barnett

²

,

Janossy

³

et al. 2000

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

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“…Still, these methods are intrinsically designed for fresh blood samples and disregard cell viability. Then again, stem cell enumeration according to ISHAGE guidelines includes a dead cell dye together with counting beads and cell definition through light scatter properties and antigen expression [14,15]. We have therefore adopted this protocol to measure the concentration of T cells.…”

Section: Introductionmentioning

confidence: 99%

ISHAGE-based single-platform flowcytometric analysis for measurement of absolute viable T cells in fresh or cryopreserved products: CD34/CD133 selected or CD3/CD19 depleted stem cells, DLI and purified CD56+CD3− NK cells

Koehl

¹

,

Bochennek

²

,

Esser

³

et al. 2007

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Exaggerated anticipatory anxiety is common in social anxiety disorder (SAD). Neuroimaging studies have revealed altered neural activity in response to social stimuli in SAD, but fewer studies have examined neural activity during anticipation of feared social stimuli in SAD. The current study examined the time course and magnitude of activity in threat processing brain regions during speech anticipation in socially anxious individuals and healthy controls (HC). Method Participants (SAD n = 58; HC n = 16) underwent functional magnetic resonance imaging (fMRI) during which they completed a 90s control anticipation task and 90s speech anticipation task.

show abstract

Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines

Cited by 457 publications

References 22 publications

Quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation

Quality of long-term cryopreserved umbilical cord blood units for hematopoietic cell transplantation

Cytofluorometric methods for assessing absolute numbers of cell subsets in blood

ISHAGE-based single-platform flowcytometric analysis for measurement of absolute viable T cells in fresh or cryopreserved products: CD34/CD133 selected or CD3/CD19 depleted stem cells, DLI and purified CD56+CD3− NK cells

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