Single-pipetting microfluidic assay device for rapid detection of Salmonella from poultry package

Smartphone-based, sensitive µPAD detection of urinary tract infection and gonorrhea

“…Previously, our group quantified the concentration of bacteria by evaluating the angle-specific Mie scatter signal from immunoagglutinated polystyrene particles (Fronczek et al, 2013;Park et al, 2013;You et al, 2011). By using paper microfluidic chips as our mPADs along with smartphone-based optical detection, E. coli was detected from waste water samples through quantifying the extent of immunoagglutination (Park and Yoon, 2015).…”

Droplet-based immunoassay on a ‘sticky’ nanofibrous surface for multiplexed and dual detection of bacteria using smartphones

“…For the anti-E. coli assay, rabbit polyclonal antibodies to the K12 antigen of E. coli (SigmaAldrich) were covalently attached to the particles (You et al, 2011). For the anti-Salmonella portion of the assay, rabbit polyclonal antibodies to the Z005 group antigen of Salmonella (Abcam Inc., Cambridge, MA, USA) were covalently coupled with the particles for 100% coverage, as previously described (Bangs Laboratories, 2008;Fronczek et al, 2013).…”

“…However, these formats often require sample pre-treatment, separate nucleic acid extraction, or separate thermocycling, and sometimes lack specificity. Recently, microfluidic-based latex particle immunoagglutination assays utilizing angle-specific Mie scatter detection have been introduced for pathogen detection in the presence of complex and biologicallyrelevant sample matrices (You et al, 2011;Fronczek et al, 2013;Stemple et al, 2014;Kim et al, 2009). These rapid, sensitive and specific platforms have demonstrated r 10 CFU mL À 1 detection limits.…”

Magnetic Bead‐Based Immunoassay Coupled with Tyramide Signal Amplification for Detection of Salmonella in Foods

“…As the cultural methods are extremely laborious and time-consuming to confirm a positive result, it is hard to quickly respond to any potential outbreak or food contamination. Alternatively, molecular and immunological approaches have been developed and used for detection of Salmonella, including polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), immunofluorescent, microfluidic assays and biosensors (Shukla et al, 2011;Fronczek et al, 2013;Brooks et al, 2014;Park et al, 2014;Rodriguez-Lazaro et al, 2014;Wu et al, 2014;Zhai et al, 2014). These alternative methods are rapid enough for positive results to be obtained within 48-72 h, and can provide low detection limits.…”