Different approaches were used to examine the function of teichoic acids (TA) as phage receptors among selected Listeria strains, and to identify and characterize specific receptor structures of host cells belonging to different serovars. This included successive removal of cell wall constituents, preparation and purification of TA, and GLC analysis of TA components. Adsorption of Listeria monocytogenes bacteriophages could be inhibited by polyvalent antisera, specific lectins and addition of purified TA. The results confirmed the necessity of TA in general and of rhamnose and glucosamine in particular for adsorption of Listeria phage Al18, which is a temperate Siphovirus (morphotype Bl), attacking predominantly serovars 1/2. Host binding of siphoviral phage A500 (predominantly lysing serovars 4b), was also dependent on cell wall TA. A phage-resistant L. monocytogenes strain was shown to lack glucosamine in its TA. These results support the view that TA substituents may play an important role not only in antigenicity of Listeria cells, but also in specificity of host recognition by two temperate Listeria phages. In contrast, the broad-host-range virulent phage A51 1 (Myovirus, morphotype A l ) uses the listerial peptidoglycan as primary receptor. This corresponds well with the observation that A511 is capable of lysing the majority of L. monocytogenes strains. , 1982;Keogh & Pettingill, 1983; Sijtsma etal., 1988; Valyasevi etal., 1994;Yokokura, 1971). In particular, cell wall teichoic acids (TA) have been found to be important for phage adsorption in Stapblococcas spp. and Bacilhs spp. (Archibald, 1976;Chatterjee, 1969; Coyette & Ghuysen, 1968; Givan e t al., 1982 ; Glaser e t al., 1966 ;Schleifer & Steber, 1974;Young, 1967 D-galactose and N-acetylglucosamine (Fiedler et al., 1984 ;Fiedler & Ruhland, 1987; Kamisango etal., 1983;Knox & Wicken, 1973 ; Ullmann & Cameron, 1969 Busse, 1990;Loessner, 1991). Propagating strains are given in parentheses. Propagation of phages was done according to Loessner & Busse (1990). For adsorption assays, host strains were grown overnight in tryptose broth (Merck) at 30 "C. Cells were washed with SM buffer (Sambrook e t al., l989), resuspended in equal volumes, and titrated. One millilitre volumes of suspensions were used for adsorption assays.
KeywordsPreparation of listerial cell walls. Broth cultures (late exponential phase) were inactivated by steaming (100 OC, 10 min), followed by concentration and washing by ultrafiltration (Sartocon module, cellulose acetate, pore size 0.2 pm, Sartorius). Finally, cells were harvested by centrifugation (10 000 g , 15 min), resuspended in SM buffer, and mechanically disrupted by double passage through a French pressure cell (SLM Aminco) at 40000 p.s.i. (276 MPa). Cell walls were prepared by methods similar to those described by Fiedler et al. (1984) and Valyasevi e t al. (1990). Crude cell walls were sedimented by differential centrifugation (1 5 000 g , 30 min), resuspended in SM buffer, and treated with DNase/RNase (3 h) and proteina...