Single or combined effects of mannan-oligosaccharides and probiotic supplements on the total oxidants, total antioxidants, enzymatic antioxidants, liver enzymes, and serum trace minerals in cyclic heat-stressed broilers

Abstract

This study was intended to explore the oxidative status of broilers under cyclic heat stress (HS) as modulated by supplementation of mannan-oligosaccharides (MOS) and a probiotic mixture (PM). Two hundred fifty 1-d-old chicks were randomly divided into 5 groups. From d 22, birds were either kept in a thermoneutral zone (TN; 26.7°C for 24 h/d) or subjected to HS (35 ± 1.1°C and 75 ± 5% RH for 8 h/d from 1000 to 1800 h) to the conclusion of the study on d 42. Birds were fed either a corn-based diet (TN and HS groups) or the same diet supplemented with 0.5% MOS (HS-MOS group), 0.1% PM (HS-PM group), or their combination as a symbiotic (SYN; HS-SYN group). On d 42, birds were killed by cervical dislocation to collect serum for the determination of total oxidants, total antioxidants, paraoxonase, arylesterase, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, and trace minerals. Heat stress increased (P < 0.05) total oxidants and total antioxidants and decreased (P < 0.05) paraoxonase and arylesterase, with no change in ceruloplasmin, aspartate aminotransferase, and alanine aminotransferase activities. Dietary supplementation decreased (P < 0.05) total oxidants and total antioxidants, with no effect on the activities of other enzymes. Heat stress did not influence serum copper, zinc, and manganese concentrations of birds when compared with those in the TN group. However, MOS increased (P < 0.05) concentrations of all the trace minerals, whereas SYN increased (P < 0.05) concentrations of only zinc and copper. It was concluded that MOS or PM supplementation, alone or as a SYN, may reduce some of the detrimental effects of HS, whereas MOS alone or as a SYN may improve the absorption of trace minerals.

Effect of dietary supplementation with &lt;i&gt;Rhizopus oryzae&lt;/i&gt; or &lt;i&gt;Chrysonilia crassa&lt;/i&gt; on growth performance, blood profile, intestinal microbial population, and carcass traits in broilers exposed to heat stress

“…As a natural protective response against oxidative stress (Sohail et al, 2011;Akbarian et al, 2016), serum total antioxidants have been reported to be increased in HS birds (Sohail et al, 2011). Conversely, HS did not significantly increase the serum antioxidant activity of broilers in the present study.…”

Serum antioxidants and trace minerals as influenced by vitamins, probiotics and proteins in broiler breeders

“…In contrast to our study, Sohail et al (2011) reported that the addition of probiotics mixture decreased serum PON1 concentration in heat-stressed broilers compared to control (thermo-neutral temperature). The difference could be attributed to the experimental condition, probiotics species, dose and duration of the experiment.…”

“…Cp has a variety of functions. It is essential for Fe homeostasis, Cu transportation, and acts as an antioxidant substance (Sohail et al 2011). In serum, Cp level is positively correlated with thiobarbituric acid reactive substances (TBARS) and negatively correlated with vitamin C (Jacobs et al 1987).…”

“…Previously, Lactobacillus and Bifadobacterium have been reported to improve the anti-oxidant status in birds (Sohail et al 2011) by decreasing the production of free radicals (Fuller 1989). The lower levels of crude protein (CP) control body weight gain and decrease blood urea, which is translated into improved reproductive performance of the birds (Zhang et al 1989;Romero-Sanchez et al 2007;Laudadio et al 2012).…”

The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment

“…In grower period, the broilers were sensitive to high temperature and humidity, thus broilers need comfortable temperature for optimum production. Comfortable temperature for optimal production in broiler chickens is 18-27 0 C (Kuczynski, 2002;Olanrewaju et al, 2010;Sohail et al, 2011;Aljuobori et al, 2016).…”