Single or Combined Dietary Supply of Psidium guajava and Phyllanthus amarus Extracts Differentially Modulate Immune Responses and Liver Proteome in Striped Catfish (Pangasianodon hyphophthalmus)

Như, Trương Quỳnh; Hằng, Bùi Thị Bích; Cornet, Valérie; Oger, Mathilde; Bạch, Lê Thị; Đào, Nguyễn Lê Anh; Hương, Đỗ Thị Thanh; Quetin-Leclercq, Joëlle; Scippo, Marie‐Louise; Phương, Nguyễn Thanh; Kestemont, Patrick

doi:10.3389/fimmu.2020.00797

Cited by 20 publications

(14 citation statements)

References 80 publications

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“…In another species of catfish known as Pangasianodon hyphophthalmus infected with E. ictaluri, several proteins were identified involve in some pathway related to immune responses, such as natural killer cell mediated cytotoxicity, antigen processing and presentation, leukocyte transendothelial migration, calcium signaling pathway, apoptosis, and nucleotide oligomerization domain, NOD-like receptor signaling pathway. The proteomics data analysis was conducted using nanoUPLC system called LC-IMS (Ion Mobility Separation)-QTOF-MS (Nhu et al, 2020).…”

Section: Proteomicsmentioning

confidence: 99%

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

et al. 2021

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Nowadays, fish production through aquaculture and wild capture is escalating due to the higher fish protein demand for human consumption. Thus, the needs for fish products to be improved in scale and quality are becoming increasingly important to meet the nutrition requirements. The negative impacts caused by pathogens infection both in hatchery and grow-out ponds, have resulted in low aquaculture productivity and economic losses for the farmers. Therefore, a systematic study exploring relevant omics datasets through a systems biology approach will enable scientist to describe the complexity and characteristics of interactions in the host-pathogen network, leading to identifying new targets or biomarkers for diseases infection caused by bacteria, viruses, and parasites. The use of different approaches and emerging technologies as a powerful tool in obtaining a new knowledge for sustainable aquaculture production has been increasingly applied by many researchers over the years. In this paper, several omics approaches used to assess the fish immune response were reviewed. A brief description is provided for the omics approaches applicable to aquacultures, such as genomics, transcriptomics, proteomics, and metabolomics. Each tool used are contributes to develop better treatment and prevention strategies to overcome aquaculture disease due to the microbial infections. In genomics and transcriptomics analysis, their parallel approach involving gene expression studies play important role in identifying immune-related genes of the host, response to infectious diseases. While for proteomics and metabolomics approaches, these omics will provide information on the biomarker discovery and understanding the immune-related biosynthetic pathway during pathogen infection. Additionally, examples of the integrative analysis of multi-omics tools required for researchers to better understand the biological systems of aquatic animals especially fish, will also be provided.

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Section: Proteomicsmentioning

confidence: 99%

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

et al. 2021

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“…Hal tersebut menyebabkan terjadinya modulasi protein dalam presentasi antigen, migrasi transendotel leukosit, sitotoksisitas yang dimediasi sel natural killer, dan jalur pensinyalan reseptor NOD pada ikan patin yang diinduksi bakteri Edwardsiella ictaluri. 24 Penelitian yang dilakukan Mohamed sinyal NF-κB, MAPK, dan PI3K-Akt. 32 Pada uji klinis yang dilakukan George et al, (2019), ekstrak etanol P. amarus standar (PHYLLPRO™) bekerja meningkatkan kadar IL-8, IL-10 yang menunjukkan efek anti-inflamasi pada cedera hati.…”

Section: Kronis Sedangkan Pelepasan Abnormal Dariunclassified

Potensi Tumbuhan Genus Phyllanthus sebagai Imunomodulator dan Antiinflamasi

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2021

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Sistem kekebalan adalah sistem pertahanan tubuh, yang bertujuan untuk mengenali dan merespons dari berbagai macam patogen atau cedera untuk melindungi tubuh. Sistem imun dapat mengalami ketidakseimbangan yang berdampak pada inflamasi yang tidak terkontrol dan menyebabkan berbagai penyakit. Dalam mengatasinya diperlukan imunomodulator dan antiinflamasi. Tumbuhan yang memiliki efek imunomodulator dan antiinflamasi yaitu tumbuhan meniran (genus Phyllanthus). Diketahui tumbuhan yang masih satu genus dengan meniran yang memiliki kemiripan kandungan kimia. Kesamaan kandungan kimia dari masing-masing tumbuhan genus Phyllanthus berkemungkinan memiliki efek dan potensi aktivitas yang sama. Tujuan dari artikel review ini adalah untuk menelusuri potensi aktivitas imunomodulator dan antiinflamasi tumbuhan genus Phyllanthus. Pencarian sumber dilakukan secara elektronik melalui situs PubMed. Berdasarkan hasil penelusuran didapatkan 43 artikel yang memenuhi kriteria penulisan. Senyawa tumbuhan genus Phyllanthus seperti: filtetralin, filantin, etil 8-hidroksi-8-metil-tridekanoat, 1,7,8-trihidroksi-2-naftaldehida, corilagin, geranin, tanin, kuersetin, rutin, dan asam galat berpotensi sebagai imunomodulator dengan cara mengatur respon imun humoral dan respon imun seluler. Senyawa tumbuhan genus Phyllanthus seperti: asam galat, filantin, hipofilantin, fisetin, katekin, corilagin, geranin, urolitin A, dan englerin A, telah terbukti berpotensi sebagai antiinflamasi dengan cara mengatur regulasi sitokin, enzim, dan protein inflamasi, serta menargetkan jalur persinyalan inflamasi.

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“…The liver of vertebrates and hepatopancreas or the digestive gland of invertebrates is of high interest in animal health research, since an important part of the function of these organs is related to energy metabolism, lipid and nutrient storage, immune response, and detoxification in the animal [8,12,26]. However, the high lipid content present in liver and hepatopancreas tissues has posed limitations to proteomics investigations, making it often necessary to modify and develop specific sample preparation protocols to enable an adequate analysis of the proteome from these organs [27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Sample Preparation Methods for Shotgun Proteomic Studies in Aquaculture Species

et al. 2021

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Proteomics has been recently introduced in aquaculture research, and more methodological studies are needed to improve the quality of proteomics studies. Therefore, this work aims to compare three sample preparation methods for shotgun LC–MS/MS proteomics using tissues of two aquaculture species: liver of turbot Scophthalmus maximus and hepatopancreas of Mediterranean mussel Mytilus galloprovincialis. We compared the three most common sample preparation workflows for shotgun analysis: filter-aided sample preparation (FASP), suspension-trapping (S-Trap), and solid-phase-enhanced sample preparations (SP3). FASP showed the highest number of protein identifications for turbot samples, and S-Trap outperformed other methods for mussel samples. Subsequent functional analysis revealed a large number of Gene Ontology (GO) terms in turbot liver proteins (nearly 300 GO terms), while fewer GOs were found in mussel proteins (nearly 150 GO terms for FASP and S-Trap and 107 for SP3). This result may reflect the poor annotation of the genomic information in this specific group of animals. FASP was confirmed as the most consistent method for shotgun proteomic studies; however, the use of the other two methods might be important in specific experimental conditions (e.g., when samples have a very low amount of protein).

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Single or Combined Dietary Supply of Psidium guajava and Phyllanthus amarus Extracts Differentially Modulate Immune Responses and Liver Proteome in Striped Catfish (Pangasianodon hyphophthalmus)

Cited by 20 publications

References 80 publications

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

Integration of Omics Tools for Understanding the Fish Immune Response Due to Microbial Challenge

Potensi Tumbuhan Genus Phyllanthus sebagai Imunomodulator dan Antiinflamasi

Comparison of Sample Preparation Methods for Shotgun Proteomic Studies in Aquaculture Species

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