Single Nucleus Rna Sequencing Profiling Identifies Distinct Cellular Populations in the Synovium of Early and Late Stage Radiographic Knee Oa

A two-stage digestion of whole murine knee joints for single-cell RNA sequencing

“…Another alternative new sequencing technology is single nuclei RNA-sequencing (sNuc-Seq). This technique processes only nuclei and the RNA found within and can be performed on frozen and undigested tissues [ 25 ], however only human synovium and porcine cartilage explants have been studied this way in the joint [ 26 , 27 ]. Techniques used to filter or only sequence parts of the joint allow a more focused sample containing a larger proportion of “desired” cells but may miss the activities of the excluded cell populations and the crosstalk that exists within the whole joint environment.…”