Single Nucleotide Polymorphisms (SNPs) Analysis of Resistant and Susceptible Sengon (Falcataria falcata) Accessions to Gall Rust Disease

Abstract

Sengon is widely planted and utilized due to its many advantages such as having high economic value, fast harvesting, easy growing and maintenance. However, sengon grown in monoculture is susceptible to gall-rust disease caused by the fungus Uromycladium falcatariae. The purpose of this study was to evaluate the growth of sengon progeny test in Kediri and application of SNPs markers on accessions of sengon progeny that are resistant and susceptible to gall-rust disease. This information is needed as a basis for tree improvement of resistant sengon strain. The total samples tested was 40 progenies from 5 resistant and 5 susceptible families with 4 progeny replications per family. Each sample was tested using 6 SNPs primers with the High Resolution Melting (HRM) method and data were analyzed using Chi-Square and Principal Component Analysist (PCA). The intensity and severity of disease occurence in resistant and susceptible families were not significantly different. The results of SNPs analysis showed that the genes were associated with sengon resistance traits. PCA analysis showed that the 6 primers used were able to separate the resistant from susceptible accessions, and thus the SNP markers can be utilized for selection of sengon gall-rust resistant lines.