Single Nucleotide Polymorphisms of FCRL3 in Iranian Patients with Behcet’s Disease

Abstract

Background: Both genetic and environmental factors influence, susceptibility to autoimmune disorders including Behcet’s disease (BD). FCRL3 (Fc receptor like 3 genes), a novel immunoregulatory gene, has recently been reported as a new promising candidate gene for general autoimmunity. This study was conducted to explore the potential association of FCRL3 polymorphisms with BD.
 Methods: This study was conducted from 2010 to 2015 in Tehran University of Medical Sciences, Tehran, Iran. Four single-nucleotide polymorphisms of FCRL3 (rs7528684, rs11264799, rs945635, and rs3761959) were genotyped in 220 patients and 220 healthy controls. Typing of the polymorphisms in this case-control study was carried out using polymerase chain reaction-restriction fragment length polymorphism analysis.
 Results: Analysis of the alleles revealed a significantly lower frequency of the A allele at the -169 site (rs7528684) in BD patients compared with that in controls (P=0.000, 66.4% versus 82%, χ2= 30.23). Moreover, a significant lower frequency of AA genotype and higher frequency of GG genotype was recorded for rs7528684. There was also relationship between posterior uveitis as a clinical sign of disease and polymorphism of allele A at the -169 site (P=0.015).
 Conclusion: This study revealed a significant difference in both allele and genotype frequency at position -169 of FCRL3 gene between Iranian patients with BD and normal subjects. These data suggest FCRL3 gene polymorphisms might be the autoimmunity risk factor for BD.

Molecular detection of oral Trichomonas tenax in periodontal disease patients by polymerase chain reaction -based 18S rRNA gene

“…The results of this study also showed that the prevalence of T. tenax was 13.48% (31 of 230) in periodontal disease patients, including 96.77% (30 of 31) in periodontitis patients and 3.23% (1 of 31) in gingivitis patients. These results were most similar to those of previous studies that reported prevalence of 14.63%, 15.5%,13.54%, and 13% (Moonmeungsan, 2004;Athari et al, 2007;Dybicz et al, 2018;Eslahi et al, 2021). Additionally, another previous study reported a global pooled prevalence of T. tenax infection of 17% (95% CI=14%-22%) (Eslahi et al, 2021).…”

“…The presence of T. tenax in periodontitis patients was higher than that in gingivitis patients, in accordance with results in other studies (Athari et al, 2007;Marty et al, 2017;Benabdelkader et al, 2019;Bracamonte-Wolf et al, 2019;Yaseen et al, 2021;Matthew et al, 2023). The explanation of the study's results could be supported by Ribeiro et al (2015) and Marty et al (2017), who revealed that the occurrence of T. tenax in the deep periodontal pocket may substantiate this role in periodontal dysbiosis.…”

Genetics of Behçet’s Disease

“…The -110 G allele and CGCG haplotype of FCRL3 were found to be associated with BD, while the ATCG haplotype was found to be protective for BD in a Chinese population [78]. In a study with Iranian BD patients, there was a significant difference demonstrated between groups at position -169 (rs7528684) of FCRL3 gene [79].…”