Single Nucleotide Polymorphisms in IL8 and TLR4 Genes as Candidates for Digital Dermatitis Resistance/Susceptibility in Holstein Cattle

Abstract

Relatedness between single nucleotide polymorphisms in IL8 and TLR4 genes and digital dermatitis resistance/susceptibility was investigated in seventy Holstein dairy cows. Animals were assigned into two groups, affected group (n = 35) and resistant group (n = 35) based on clinical signs and previous history of farm clinical records. Blood samples were collected for DNA extraction to ampliy fragments of 267-bp and 382-bp for IL8 and TLR4 genes, respectively. PCR-DNA sequencing revealed three SNPs in each of IL8 and TLR4 genes. The identified SNPs associated with digital dermatitis resistance were C94T, A220G, and T262A for IL8 and C118T for TLR4. However, the G349C and C355A SNPs in TLR4 gene were associated with digital dermatitis susceptibility. Chi-square analysis for comparison the distribution of all identified SNPs in both IL8 and TLR4 genes between resistant and affected animals showed no significant variation among the identified SNPs in IL8 gene. Meanwhile, there was a significant variation in case of TLR4 gene. As a pilot study, the present results revealed that identified SNPs in IL8 and TLR4 genes can be used as a genetic marker and predisposing factor for resistance/susceptibility to digital dermatitis in dairy cows. However, TLR4 gene may be a potential candidate for such disease.

The outer membrane protein Tp92 of Treponema pallidum induces human mononuclear cell death and IL‐8 secretion

“…This scenario favours the survival of T. pallidum in the hosts and prevents the removal of T. pallidum by strong inflammatory responses. Meanwhile, Tp92 induces the production of IL‐8, which causes the accumulation of neutrophils and lymphocytes to the site of infection, increases the degree of infiltration of inflammatory cells in the infected area, and aggravates the immunopathological damage of the site . On the other hand, IL‐8 promotes the release of elastase by neutrophils, damages endothelial cells, and facilitates the transport of T. pallidum across the endothelial barrier and into the blood .…”

“…62 The T. pallidum antigen TpF1 promotes the secretion of the anti-inflammatory factor IL-10 to resist elimination by inflammation. 63 Interestingly, further research showed that Tp92 64 On the other hand, IL-8 promotes the release of elastase by neutrophils, damages endothelial cells, and facilitates the transport of T. pallidum across the endothelial barrier and into the blood. 65 The TpF1 protein induces the secretion of IL-8 by hosts, promotes skin and mucosal vascular proliferation in patients with syphilis, increases vascular permeability, and helps the spread of T. pallidum throughout the body via the blood vessels.…”

Genetic Polymorphisms inLTF/EcoRI andTLR4/AluI loci as candidates for milk and reproductive performance assessment in Holstein cattle

“…Several polymorphisms have been identified in bovine TLR4 gene (White et al, ) which are reported to affect SCS, be associated with estimated breeding values for lactation persistency and also revealed an association of E3+2021 with milk fat and protein percentage in late lactation in Holstein‐Friesian, Jersey, Norwegian Red, Montbeliarde and Holstein‐Friesian × Jersey cows (Beecher et al, ; Sharma, Leyva, Schenkel, & Karrow, ; Wang, Xu, Gao, Ren, & Chen, ). Many studies reported the association between bovine TLR4 gene and somatic cell score and susceptibility/resistance of many dairy cow diseases (El‐Shafaey et al, ; Li et al, ; Noori et al, ); however, few literatures reported such association with reproductive performance traits (Goldammer et al, ; Shimizu et al, ; Werling & Jungi, ).…”

Genetic polymorphisms and expression patterns of TLR4 gene in commercial broiler breeds

“…Recent advances in molecular genetics and development of molecular techniques have led to the discovery of genes, or markers associated with genes involved in economic traits (GAO et al, 2007;GHANEM et al, 2016). When a polymorphic locus is associated with the economic traits of animals, that polymorphic locus can therefore be used as a genetic marker for the trait and become useful as a selection criterion for genetic improvement of this trait (marker-assisted selection) (EL-SHAFAEY et al, 2017;ELDOMANY et al, 2019).…”