Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Alam, Mahmuda Bilkis Bintee; Omar, Abdullah Ibne; Faruque, Md. Omar; Notter, D. R.; Kathiravan, P.; Mondal, M. M. H.; Sarder, Md. Jalal Uddin; Shamsuddin, Md.; Cao, Jianhua; Du, Xiaoyong; Wu, Zhenyang; Zhao, Shuhong

doi:10.1186/s40104-019-0327-8

Cited by 11 publications

(8 citation statements)

References 52 publications

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“…Small ruminant farming industry contributes one of the major means to develop living standards in numerous developing countries and plays an important role in the national economy [1]. Among small ruminant species, goats are particularly vulnerable to gastrointestinal nematodes in humid, tropical, and subtropical regions and are particularly overwhelmed by Haemonchus contortus ( H. contortus ) [2].…”

Section: Introductionmentioning

confidence: 99%

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Combined Use of Indirect ELISA and Western Blotting with Recombinant Hepatocellular Carcinoma-Associated Antigen 59 Is a Potential Immunodiagnostic Tool for the Detection of Prepatent Haemonchus contortus Infection in Goat

Naqvi

Memon

et al. 2019

Animals

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Haemonchus contortus is recognized as one of the important health problems in small ruminants, leading to reduced production and economic loss for farmers worldwide. Prepatent diagnosis of H. contortus infection is crucial to improve control strategies as this helminth may remove up to one-fifth of total erythrocytes and may cause anemia, edema, diarrhea, and ultimately death in young animals. In this study, one of the excretory and secretory products, rHc-HCA59, was purified and used as antigen to detect specific antibodies in H. contortus infected goats during prepatent stage of infection using indirect enzyme linked immunosorbent assay (ELISA) as screening test. All goats (n = 38) were housed indoor, experimentally infected with 8000 infective larvae (L3) of H. contortus, and serum samples were collected prior to infection and at 14th day of infection. Immunoblotting was performed to confirm the results of indirect ELISA, evaluate the cross reactivity against rHc-HCA59 in sera of most common co-infecting parasites and rectify the false negative samples. Furthermore, three different batches of rHc-HCA59 were produced to evaluate the repeatability of ELISA. No eggs were detected in feces of all goats collected at 7th and 14th day of infection but, H. contortus eggs were detected at 21 days post infection in the feces. Indirect ELISA performed in this study showed 87% sensitivity and 100% specificity. The western blot analysis confirmed immunoreactivity in serum samples which scored positive in indirect ELISA and recognized the samples as negative which had OD450 lower than negative cut-off value in indirect ELISA. Furthermore, all false negative sera (n = 5) that had OD450 value between positive and negative cut-off value in rHc-HCA59 based ELISA were clearly positive in western blot. Moreover, no cross-reactivity was detected in ELISA and western blotting against rHc-HCA59 in positive sera of Toxoplasma gondii, Fasciola hepatica, and Trichinella spiralis. The results of this study concluded that combined use of indirect ELISA and western blotting with rHc-HCA59 is a potential immunodiagnostic tool for the detection of H. contortus infection during prepatent period in goats.

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Section: Introductionmentioning

confidence: 99%

“…It is an important blood sucking parasite found in abomasum that may remove about 0.05 mL of blood per day by seepage from lesions [3]. H. contortus infection leads to significant economic losses to small ruminant farming industry as a consequence of high mortality and morbidity [1].…”

Section: Introductionmentioning

confidence: 99%

Combined Use of Indirect ELISA and Western Blotting with Recombinant Hepatocellular Carcinoma-Associated Antigen 59 Is a Potential Immunodiagnostic Tool for the Detection of Prepatent Haemonchus contortus Infection in Goat

Naqvi

Memon

et al. 2019

Animals

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“…Contrary to our expectation and despite serial founder events throughout the species reintroduction history, the three studied Tlr genes and the SlC11A1 have all maintained several haplotypes while the MHC Class II DRB (second exon) is monomorphic in Bargy. There are very few studies that investigate Tlr polymorphism in ungulates populations: levels of Tlr haplotype diversity in Bargy ibex (N H = 3-5, H D ) appears similar to those reported for the same genes in roe deer populations (N H = 3-5) 17 , domestic goat (N H = 1-4) 55 or cattle (N H = 2-5) 56 . Tlr4 showed four functional haplotypes (i.e.…”

Section: Discussionmentioning

confidence: 66%

Genetic epidemiology of the Alpine ibex reservoir of persistent and virulent brucellosis outbreak

Quéméré

Rossi

Petit³

et al. 2020

Sci Rep

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While it is now broadly accepted that inter-individual variation in the outcomes of host–pathogen interactions is at least partially genetically controlled, host immunogenetic characteristics are rarely investigated in wildlife epidemiological studies. Furthermore, most immunogenetic studies in the wild focused solely on the major histocompatibility complex (MHC) diversity despite it accounts for only a fraction of the genetic variation in pathogen resistance. Here, we investigated immunogenetic diversity of the Alpine ibex (Capra ibex) population of the Bargy massif, reservoir of a virulent outbreak of brucellosis. We analysed the polymorphism and associations with disease resistance of the MHC Class II Drb gene and several non-MHC genes (Toll-like receptor genes, Slc11A1) involved in the innate immune response to Brucella in domestic ungulates. We found a very low neutral genetic diversity and a unique MHC Drb haplotype in this population founded few decades ago from a small number of individuals. By contrast, other immunity-related genes have maintained polymorphism and some showed significant associations with the brucellosis infection status hence suggesting a predominant role of pathogen-mediated selection in their recent evolutionary trajectory. Our results highlight the need to monitor immunogenetic variation in wildlife epidemiological studies and to look beyond the MHC.

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“…and some narrative reviews mentioning the lack of data on MALDI-TOF in helminthology [32, 66, 67]. Yet, most studies focused on distinct analyses of specific components, such as peptides [66–86], proteins [69, 87–114], lipids [61, 62, 115–124], carbohydrates [125–143] and nucleic acids [144] in a research context. Hence, MALDI-TOF was mainly applied to study and compare the proteome or the peptidome of different helminth species, and most reports focused on C. elegans .…”

Section: Resultsmentioning

confidence: 99%

MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review

et al. 2019

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Background Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory. Thus far, only few attempts have been made to apply the technique in clinical parasitology, particularly regarding helminth identification. Methods We systematically reviewed the scientific literature on studies pertaining to MALDI-TOF MS as a diagnostic technique for helminths (cestodes, nematodes and trematodes) of medical and veterinary importance. Readily available electronic databases (i.e. PubMed/MEDLINE, ScienceDirect, Cochrane Library, Web of Science and Google Scholar) were searched from inception to 10 October 2018, without restriction on year of publication or language. The titles and abstracts of studies were screened for eligibility by two independent reviewers. Relevant articles were read in full and included in the systematic review. Results A total of 84 peer-reviewed articles were considered for the final analysis. Most papers reported on the application of MALDI-TOF for the study of Caenorhabditis elegans , and the technique was primarily used for identification of specific proteins rather than entire pathogens. Since 2015, a small number of studies documented the successful use of MALDI-TOF MS for species-specific identification of nematodes of human and veterinary importance, such as Trichinella spp. and Dirofilaria spp. However, the quality of available data and the number of examined helminth samples was low. Conclusions Data on the use of MALDI-TOF MS for the diagnosis of helminths are scarce, but recent evidence suggests a potential role for a reliable identification of nematodes. Future research should explore the diagnostic accuracy of MALDI-TOF MS for identification of (i) adult helminths, larvae and eggs shed in faecal samples; and (ii) helminth-related proteins that are detectable in serum or body fluids of infected individuals. Electronic supplementary material The online version of this article (10.1186/s13071-019-3493-9) contains supplementary material, which is available to authorized users.

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Single nucleotide polymorphisms in candidate genes are significantly associated with resistance to Haemonchus contortus infection in goats

Cited by 11 publications

References 52 publications

Combined Use of Indirect ELISA and Western Blotting with Recombinant Hepatocellular Carcinoma-Associated Antigen 59 Is a Potential Immunodiagnostic Tool for the Detection of Prepatent Haemonchus contortus Infection in Goat

Combined Use of Indirect ELISA and Western Blotting with Recombinant Hepatocellular Carcinoma-Associated Antigen 59 Is a Potential Immunodiagnostic Tool for the Detection of Prepatent Haemonchus contortus Infection in Goat

Genetic epidemiology of the Alpine ibex reservoir of persistent and virulent brucellosis outbreak

MALDI-TOF mass spectrometry as a diagnostic tool in human and veterinary helminthology: a systematic review

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